Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PE064_RS03165 Genome accession   NZ_CP116007
Coordinates   646948..648084 (+) Length   378 a.a.
NCBI ID   WP_162110521.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain DMF-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641948..653084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE064_RS03140 (PE064_03140) - 642208..643413 (-) 1206 WP_271250607.1 cystathionine gamma-synthase -
  PE064_RS03145 (PE064_03145) - 643513..644883 (-) 1371 WP_271250608.1 pyridoxal-phosphate dependent enzyme -
  PE064_RS03150 (PE064_03150) - 644998..645216 (-) 219 WP_162110524.1 YdcH family protein -
  PE064_RS03155 (PE064_03155) - 645573..646460 (-) 888 WP_271250609.1 OmpA family protein -
  PE064_RS03160 (PE064_03160) - 646457..646852 (-) 396 WP_271250610.1 DUF4398 domain-containing protein -
  PE064_RS03165 (PE064_03165) pilU 646948..648084 (+) 1137 WP_162110521.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PE064_RS03170 (PE064_03170) maiA 648132..648791 (-) 660 WP_187574319.1 maleylacetoacetate isomerase -
  PE064_RS03175 (PE064_03175) - 648890..649879 (-) 990 WP_162110519.1 fumarylacetoacetate hydrolase family protein -
  PE064_RS03180 (PE064_03180) - 649942..650679 (-) 738 WP_187574317.1 2OG-Fe dioxygenase family protein -
  PE064_RS03190 (PE064_03190) rpsI 650864..651256 (-) 393 WP_162110517.1 30S ribosomal protein S9 -
  PE064_RS03195 (PE064_03195) rplM 651259..651687 (-) 429 WP_055936916.1 50S ribosomal protein L13 -
  PE064_RS03200 (PE064_03200) coq7 651875..652516 (+) 642 WP_271250611.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  PE064_RS03205 (PE064_03205) sugE 652628..652948 (-) 321 WP_271250612.1 quaternary ammonium compound efflux SMR transporter SugE -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41781.15 Da        Isoelectric Point: 5.9760

>NTDB_id=774873 PE064_RS03165 WP_162110521.1 646948..648084(+) (pilU) [Pseudoxanthomonas mexicana strain DMF-5]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPVFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELNLPPVLKDIIMTPRGLVLIVGSTGSGKSTSLASMIDYRNTTSTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRLPAAEVLINTPMIRDLLRRGQVHEIKAAMEESLEEGMESFDQCL
FRMVKEGLIEQEEALRAADSRDGLALKFRLSEGSKGEHDPYADYDTGSASPRITHGFG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=774873 PE064_RS03165 WP_162110521.1 646948..648084(+) (pilU) [Pseudoxanthomonas mexicana strain DMF-5]
ATGGACATCGGCTATTTCCTGAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTTCTGACCACCGGGGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCGTTGGGCAATACCGGCCTGCCGCCCGGTATGGTCAAGAAGATCGCGTATTCGC
TGATGGACGAGGGCCAGGTGCCCGTGTTCGAGCGCGAGCTCGAGCTGAACATGGCCATCGCCCTGCAGGACGCCGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTCATCCGTGCCATCCGCAGCGTGATCCCCAGCATCGA
GGAGCTGAACCTGCCGCCGGTGTTGAAGGACATCATCATGACGCCGCGCGGGCTGGTGCTGATCGTGGGGTCCACCGGCT
CGGGCAAGTCGACGTCGCTGGCCTCGATGATCGACTACCGCAACACCACCAGCACTGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGCAGTCCATCGTCAACCAGCGCGAAGTGGGCCTGGACACCCATGCCTTCCACAA
TGCGTTGAAGAACGCCATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTGGACGCCACCACGATGGAGGCGG
CCATCGCCTTCGCCGAAACAGGCCACCTCTGCCTGGCCACGCTGCACTCGAACAATGCGGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTCTCGCAGCG
CCTGGTGAAAGGCGTCGACGGCCGCCGCCTGCCCGCCGCCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGGGGGCAGGTGCACGAGATCAAGGCCGCCATGGAGGAGTCGCTGGAGGAAGGCATGGAGTCCTTCGACCAGTGCCTG
TTCCGGATGGTGAAGGAAGGGCTGATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGACTCGCGCGACGGTCTGGCCCTGAA
GTTCCGCCTGTCCGAGGGCAGCAAGGGCGAACACGACCCCTACGCCGACTACGACACCGGCAGCGCCTCGCCCCGCATCA
CCCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

94.974

0.492
  pilU Acinetobacter baylyi ADP1

49.153

93.651

0.46
  pilU Vibrio cholerae strain A1552

47.154

97.619

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.297

99.735

0.442
  pilT Pseudomonas aeruginosa PAK

42.012

89.418

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.418

0.368
  pilT Acinetobacter baylyi ADP1

40.588

89.947

0.365
  pilT Acinetobacter baumannii D1279779

40.828

89.418

0.365
  pilT Acinetobacter nosocomialis M2

40.828

89.418

0.365
  pilT Acinetobacter baumannii strain A118

40.828

89.418

0.365
  pilT Legionella pneumophila strain Lp02

40.533

89.418

0.362
  pilT Legionella pneumophila strain ERS1305867

40.533

89.418

0.362