Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H7K64_RS01750 Genome accession   NZ_AP022227
Coordinates   374929..376164 (+) Length   411 a.a.
NCBI ID   WP_420029540.1    Uniprot ID   -
Organism   Pseudomonas putida strain WP8-W18-CRE-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369929..381164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K64_RS01735 (WP8W18C01_03390) pilM 372503..373423 (+) 921 WP_182816994.1 type IV pilus biogenesis protein PilM -
  H7K64_RS01740 (WP8W18C01_03400) - 373423..373959 (+) 537 WP_090343357.1 PilN domain-containing protein -
  H7K64_RS01745 (WP8W18C01_03410) - 373956..374972 (+) 1017 WP_232092097.1 pilus assembly protein PilP -
  H7K64_RS01750 (WP8W18C01_03420) pilQ 374929..376164 (+) 1236 WP_420029540.1 type IV pilus secretin PilQ Machinery gene
  H7K64_RS01755 (WP8W18C01_03430) aroK 376169..376687 (+) 519 WP_043208585.1 shikimate kinase AroK -
  H7K64_RS01760 (WP8W18C01_03440) aroB 376785..377882 (+) 1098 WP_182816998.1 3-dehydroquinate synthase -
  H7K64_RS01765 (WP8W18C01_03450) - 377898..379535 (+) 1638 WP_043208580.1 AAA family ATPase -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44558.04 Da        Isoelectric Point: 5.1134

>NTDB_id=77262 H7K64_RS01750 WP_420029540.1 374929..376164(+) (pilQ) [Pseudomonas putida strain WP8-W18-CRE-01]
MLSSPAATASLYRGEPLSLNFQDIEVRAALQVLADHAGINLVASDTVQGSLTLRLEDVPWDQALDLVLRSKGLARREEGN
VLVVAPIAELAEQFREARSGQALDAQLQPMRRELLPIHHAKAADMAELLLAALADEGILTGRGSLSVDPRTNTLVAYQPA
DRLAELRRLVGQLDVPVRQVLIEARIVEANVDYEKSLGVRWGAPLQGDTARLGKELFVDLGVERGTSGIGLGVLRGGTLL
DLELSAMEKSGNGEIISQPKVVTADKETARIIKGTEVPYQETSKSGATSISFREASLSLEVTPQITPDGKVVMAVRVTKD
EPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFLGDLPYVGRLFRRDVLREKKSELLVFLTPR
IMSDQAIAVSR

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=77262 H7K64_RS01750 WP_420029540.1 374929..376164(+) (pilQ) [Pseudomonas putida strain WP8-W18-CRE-01]
ATGCTGTCGAGCCCGGCGGCGACAGCATCGCTTTATCGAGGTGAGCCGCTGTCCCTGAATTTCCAGGACATCGAAGTGCG
CGCGGCGCTGCAGGTGCTGGCCGATCATGCCGGCATCAACTTGGTTGCCAGCGACACGGTACAAGGCAGTCTTACCTTGC
GCCTGGAAGACGTGCCCTGGGATCAGGCGCTGGACCTTGTATTGCGCAGCAAAGGACTGGCCCGGCGCGAGGAGGGTAAC
GTTCTGGTGGTCGCGCCCATCGCGGAGCTGGCAGAGCAGTTTCGTGAAGCCCGGTCTGGGCAGGCGCTGGATGCGCAATT
GCAGCCTATGCGGCGTGAGCTGCTGCCGATCCATCACGCCAAGGCGGCGGACATGGCAGAGTTGTTGTTGGCCGCGCTCG
CCGACGAGGGCATTCTCACCGGGCGCGGCAGCCTGAGTGTCGACCCACGGACCAACACCTTGGTGGCCTACCAGCCGGCC
GACCGACTCGCCGAGTTGCGGCGACTGGTGGGGCAGCTGGATGTGCCGGTGCGACAAGTGCTGATCGAGGCGCGGATCGT
CGAGGCCAACGTCGATTACGAAAAAAGCCTGGGTGTGCGCTGGGGCGCGCCCCTGCAAGGCGATACCGCTCGTCTGGGAA
AAGAACTGTTCGTCGACCTGGGTGTGGAGCGGGGGACGTCCGGCATTGGGTTGGGGGTGCTACGAGGCGGGACGCTGCTG
GACTTGGAGCTCAGTGCCATGGAAAAGAGCGGAAACGGCGAGATCATCTCCCAGCCGAAGGTGGTCACCGCTGACAAGGA
GACCGCGCGGATCATCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCAAGAGTGGCGCGACCTCCATCTCGTTTCGCG
AGGCATCGTTATCGCTTGAGGTGACGCCGCAGATCACGCCCGATGGCAAAGTCGTGATGGCTGTCAGGGTGACCAAGGAC
GAGCCTGACTACGTCAATGCACTGAACAACGTCCCGCCCATCCGCAAGAATGAGGTCAACGCCAAGGTGCGGGTGGCGGA
CGGCGAGACGATCGTCATCGGTGGCGTTTACTCGACCACGCAAAACAATGTGGTCGACAAGGTGCCATTTTTAGGCGATC
TGCCGTATGTTGGCAGGCTGTTTCGACGCGATGTATTACGAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGT
ATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(40-85)

Bacterial type II and III secretion system protein (Secretin)

(246-402)

Bacterial type II/III secretion system short domain (Secretin_N)

(114-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

60.092

100

0.637
  comQ Acinetobacter baylyi ADP1

43.43

100

0.474
  pilQ Acinetobacter baumannii strain A118

41.703

100

0.465
  pilQ Acinetobacter baumannii D1279779

41.703

100

0.465
  pilQ Legionella pneumophila strain Lp02

43.437

100

0.443
  pilQ Legionella pneumophila strain ERS1305867

43.437

100

0.443
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.584

100

0.433
  pilQ Vibrio cholerae strain A1552

42.584

100

0.433
  pilQ Vibrio campbellii strain DS40M4

40.662

100

0.418
  pilQ Neisseria gonorrhoeae strain FA1090

35.646

100

0.363
  pilQ Neisseria meningitidis MC58

35.646

100

0.363
  pilQ Neisseria meningitidis M1080

35.646

100

0.363

Multiple sequence alignment