Detailed information NTG0771146

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: PCP87_RS16290
• Genome accession: NZ_CP115277
• Coordinates: 3449177..3449629 (+)
• Length: 150 a.a.
• NCBI ID: WP_024928732.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain F012
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3444177..3454629

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP87_RS16265 (PCP87_16280)	yacG	3444340..3444540 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PCP87_RS16270 (PCP87_16285)	coaE	3444537..3445148 (-)	612	WP_010793792.1	dephospho-CoA kinase	-
PCP87_RS16275 (PCP87_16290)	pilD	3445145..3446017 (-)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PCP87_RS16280 (PCP87_16295)	pilC	3446021..3447241 (-)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
PCP87_RS16285 (PCP87_16300)	pilB	3447245..3448945 (-)	1701	WP_010793793.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCP87_RS16290 (PCP87_16305)	pilA	3449177..3449629 (+)	453	WP_024928732.1	pilin	Machinery gene
PCP87_RS16300 (PCP87_16315)	-	3449884..3450126 (-)	243	WP_023082027.1	hypothetical protein	-
PCP87_RS16305 (PCP87_16320)	-	3451155..3451414 (-)	260	Protein_3222	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
PCP87_RS16310 (PCP87_16325)	fliR	3451417..3452199 (-)	783	Protein_3223	flagellar biosynthetic protein FliR	-
PCP87_RS16315 (PCP87_16330)	fliQ	3452199..3452468 (-)	270	WP_023435365.1	flagellar biosynthesis protein FliQ	-
PCP87_RS16320 (PCP87_16335)	fliP	3452513..3453268 (-)	756	WP_031285507.1	flagellar type III secretion system pore protein FliP	-
PCP87_RS16325 (PCP87_16340)	-	3453548..3454210 (-)	663	WP_033992423.1	DUF3218 family protein	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15413.63 Da
• Isoelectric Point: 8.4829

>NTDB_id=771146 PCP87_RS16290 WP_024928732.1 3449177..3449629(+) (pilA) [Pseudomonas aeruginosa strain F012]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTADTTYV
GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide

• Length: 453 bp

>NTDB_id=771146 PCP87_RS16290 WP_024928732.1 3449177..3449629(+) (pilA) [Pseudomonas aeruginosa strain F012]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGCAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	68.456	99.333	0.68
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.333	100	0.473
pilA	Vibrio parahaemolyticus RIMD 2210633	46.939	98	0.46
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.671	100	0.46
pilA	Vibrio cholerae strain A1552	43.671	100	0.46
pilA	Vibrio cholerae C6706	43.671	100	0.46
pilA	Acinetobacter baumannii strain A118	44.295	99.333	0.44
comP	Acinetobacter baylyi ADP1	41.558	100	0.427
pilA2	Legionella pneumophila strain ERS1305867	42.282	99.333	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.333	0.413
pilA2	Legionella pneumophila str. Paris	41.611	99.333	0.413