Detailed information NTG0771145

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PCP87_RS16285
• Genome accession: NZ_CP115277
• Coordinates: 3447245..3448945 (-)
• Length: 566 a.a.
• NCBI ID: WP_010793793.1
• Uniprot ID: A0A367MDN5
• Organism: Pseudomonas aeruginosa strain F012
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3442245..3453945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP87_RS16245 (PCP87_16260)	-	3442410..3442835 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
PCP87_RS16250 (PCP87_16265)	-	3442832..3443302 (+)	471	WP_010793791.1	hypothetical protein	-
PCP87_RS16255 (PCP87_16270)	-	3443299..3443988 (+)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
PCP87_RS16260 (PCP87_16275)	-	3444094..3444303 (+)	210	WP_003094660.1	hypothetical protein	-
PCP87_RS16265 (PCP87_16280)	yacG	3444340..3444540 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PCP87_RS16270 (PCP87_16285)	coaE	3444537..3445148 (-)	612	WP_010793792.1	dephospho-CoA kinase	-
PCP87_RS16275 (PCP87_16290)	pilD	3445145..3446017 (-)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PCP87_RS16280 (PCP87_16295)	pilC	3446021..3447241 (-)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
PCP87_RS16285 (PCP87_16300)	pilB	3447245..3448945 (-)	1701	WP_010793793.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCP87_RS16290 (PCP87_16305)	pilA	3449177..3449629 (+)	453	WP_024928732.1	pilin	Machinery gene
PCP87_RS16300 (PCP87_16315)	-	3449884..3450126 (-)	243	WP_023082027.1	hypothetical protein	-
PCP87_RS16305 (PCP87_16320)	-	3451155..3451414 (-)	260	Protein_3222	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
PCP87_RS16310 (PCP87_16325)	fliR	3451417..3452199 (-)	783	Protein_3223	flagellar biosynthetic protein FliR	-
PCP87_RS16315 (PCP87_16330)	fliQ	3452199..3452468 (-)	270	WP_023435365.1	flagellar biosynthesis protein FliQ	-
PCP87_RS16320 (PCP87_16335)	fliP	3452513..3453268 (-)	756	WP_031285507.1	flagellar type III secretion system pore protein FliP	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62354.24 Da
• Isoelectric Point: 5.8580

>NTDB_id=771145 PCP87_RS16285 WP_010793793.1 3447245..3448945(-) (pilB) [Pseudomonas aeruginosa strain F012]
MNNSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=771145 PCP87_RS16285 WP_010793793.1 3447245..3448945(-) (pilB) [Pseudomonas aeruginosa strain F012]
ATGAACAACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCTTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCTATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

3D structure

• AlphaFold DB: A0A367MDN5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.517	100	0.581
pilB	Acinetobacter baylyi ADP1	57.194	99.47	0.569
pilB	Legionella pneumophila strain ERS1305867	55.124	100	0.551
pilB	Vibrio parahaemolyticus RIMD 2210633	50.887	99.647	0.507
pilF	Neisseria gonorrhoeae MS11	50.534	99.293	0.502
pilB	Vibrio cholerae strain A1552	50	99.647	0.498
pilB	Vibrio campbellii strain DS40M4	49.645	99.647	0.495
pilF	Thermus thermophilus HB27	39.572	99.117	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.417	93.11	0.376
pilB/pilB1	Synechocystis sp. PCC 6803	38.764	94.346	0.366