NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	OZ175_RS17460	Genome accession	NZ_CP113974
Coordinates	3723755..3724207 (-)	Length	150 a.a.
NCBI ID	WP_024947914.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain M6A146
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3718755..3729207

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OZ175_RS17440 (OZ175_17440)	ampD	3719461..3720027 (-)	567	WP_268858243.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
OZ175_RS17445 (OZ175_17445)	-	3720178..3722433 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
OZ175_RS17450 (OZ175_17450)	nadC	3722719..3723567 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OZ175_RS17460 (OZ175_17460)	pilA	3723755..3724207 (-)	453	WP_024947914.1	pilin	Machinery gene
OZ175_RS17465 (OZ175_17465)	pilB	3724436..3726136 (+)	1701	WP_009875880.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OZ175_RS17470 (OZ175_17470)	pilC	3726140..3727360 (+)	1221	WP_016253888.1	type 4a pilus biogenesis protein PilC	Machinery gene
OZ175_RS17475 (OZ175_17475)	pilD	3727364..3728236 (+)	873	Protein_3460	type IV prepilin peptidase/methyltransferase PilD	-
OZ175_RS17480 (OZ175_17480)	coaE	3728233..3728844 (+)	612	WP_268858244.1	dephospho-CoA kinase	-
OZ175_RS17485 (OZ175_17485)	yacG	3728841..3729041 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15622.02 Da Isoelectric Point: 8.4827

                                >NTDB_id=763914 OZ175_RS17460 WP_024947914.1 3723755..3724207(-) (pilA) [Pseudomonas aeruginosa strain M6A146]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG

VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide

Download Length: 453 bp

                                >NTDB_id=763914 OZ175_RS17460 WP_024947914.1 3723755..3724207(-) (pilA) [Pseudomonas aeruginosa strain M6A146]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT

TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG

AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG

GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC

TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA

AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	100	100	1
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.682	100	0.48
pilA	Acinetobacter baumannii strain A118	45.333	100	0.453
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.575	97.333	0.453
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.675	100	0.447
pilA	Vibrio cholerae C6706	42.675	100	0.447
pilA	Vibrio cholerae strain A1552	42.675	100	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	48.507	89.333	0.433
comP	Acinetobacter baylyi ADP1	40.789	100	0.413
pilA2	Legionella pneumophila strain ERS1305867	39.865	98.667	0.393
pilA/pilA1	Eikenella corrodens VA1	39.456	98	0.387
pilA2	Legionella pneumophila str. Paris	39.189	98.667	0.387

Detailed information NTG0763914