Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OY559_RS03445 Genome accession   NZ_CP113783
Coordinates   756131..757270 (-) Length   379 a.a.
NCBI ID   WP_277728720.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. SE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 751131..762270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OY559_RS03405 (OY559_03405) sugE 751227..751541 (+) 315 WP_277728715.1 quaternary ammonium compound efflux SMR transporter SugE -
  OY559_RS03410 (OY559_03410) coq7 751683..752324 (-) 642 WP_277728716.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  OY559_RS03415 (OY559_03415) rplM 752517..752945 (+) 429 WP_055936916.1 50S ribosomal protein L13 -
  OY559_RS03420 (OY559_03420) rpsI 752948..753340 (+) 393 WP_142123406.1 30S ribosomal protein S9 -
  OY559_RS03430 (OY559_03430) - 753526..754263 (+) 738 WP_277728717.1 2OG-Fe dioxygenase family protein -
  OY559_RS03435 (OY559_03435) - 754327..755316 (+) 990 WP_277728718.1 fumarylacetoacetate hydrolase family protein -
  OY559_RS03440 (OY559_03440) maiA 755415..756077 (+) 663 WP_277728719.1 maleylacetoacetate isomerase -
  OY559_RS03445 (OY559_03445) pilU 756131..757270 (-) 1140 WP_277728720.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OY559_RS03450 (OY559_03450) - 757365..757760 (+) 396 WP_277728721.1 DUF4398 domain-containing protein -
  OY559_RS03455 (OY559_03455) - 757757..758653 (+) 897 WP_277728722.1 OmpA family protein -
  OY559_RS03460 (OY559_03460) - 759010..759228 (+) 219 WP_142123411.1 YdcH family protein -
  OY559_RS03465 (OY559_03465) - 759344..760714 (+) 1371 WP_277728723.1 pyridoxal-phosphate dependent enzyme -
  OY559_RS03470 (OY559_03470) - 760813..762006 (+) 1194 WP_277728724.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41961.25 Da        Isoelectric Point: 5.9760

>NTDB_id=763033 OY559_RS03445 WP_277728720.1 756131..757270(-) (pilU) [Pseudoxanthomonas sp. SE1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPVFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELNLPPVLKDIIMTPRGLVLIVGSTGSGKSTSLASMIDYRNTTSTGHILTIED
PIEYLHRHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGTDGRRMPAAEVLINTPMIRDLLRRGQVHEIKAAMEESLEEGMETFDQCL
FRMVKEGQIEQEEALRAADSRDGLALKFRLSEGSKGEHDPYADYDTGSSSSPRITHGFG

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=763033 OY559_RS03445 WP_277728720.1 756131..757270(-) (pilU) [Pseudoxanthomonas sp. SE1]
ATGGACATCGGCTATTTCCTGAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTTCTCACCACCGGGGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTCTATCCCTTGGGCAATACCGGCCTGCCGCCGGGCATGGTGAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCGTGTTCGAGCGCGAACTGGAGCTCAACATGGCCATCGCCCTGCAGGACGCAGGGCGC
TTCCGCGTCAACGTCTTCAAGCAGCGCGGTGAGGTGGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCCAGCATCGA
AGAGCTGAACCTGCCGCCGGTGCTGAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGATTGTGGGCTCCACCGGAT
CGGGCAAGTCGACCTCGCTCGCCTCGATGATCGACTATCGCAACACCACCAGCACCGGCCATATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAGGCATAAGCAGTCGATCGTCAACCAGCGCGAAGTGGGCCTGGACACGCATGCGTTCCACAA
CGCACTGAAGAATGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTGGACGCCACCACGATGGAGGCCG
CCATCGCCTTCGCCGAAACCGGCCATCTGTGCCTGGCGACACTGCACTCGAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTATTGATGAACCTGGCGCTCAACCTGCGCGCCGTCGTATCCCAACG
CCTGGTGAAGGGTACCGACGGGCGTCGCATGCCGGCGGCGGAAGTGTTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGGCAGGTGCACGAGATCAAGGCCGCCATGGAGGAATCGCTGGAGGAAGGCATGGAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGGAGGGCCAGATCGAGCAGGAGGAAGCGCTGCGCGCAGCGGACTCGCGCGACGGCCTGGCACTGAA
GTTCCGCCTGTCCGAAGGCAGCAAGGGCGAACACGACCCTTACGCCGACTACGACACCGGCAGCAGCAGTTCGCCGCGCA
TCACGCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

94.723

0.493
  pilU Vibrio cholerae strain A1552

46.933

98.945

0.464
  pilU Acinetobacter baylyi ADP1

49.435

93.404

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.301

100

0.451
  pilT Pseudomonas aeruginosa PAK

42.012

89.182

0.375
  pilT Acinetobacter baumannii strain A118

41.124

89.182

0.367
  pilT Acinetobacter nosocomialis M2

41.124

89.182

0.367
  pilT Acinetobacter baumannii D1279779

41.124

89.182

0.367
  pilT Acinetobacter baylyi ADP1

40.882

89.71

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.182

0.364
  pilT Legionella pneumophila strain Lp02

40.828

89.182

0.364
  pilT Legionella pneumophila strain ERS1305867

40.828

89.182

0.364