Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GZH91_RS15335 Genome accession   NZ_AP021884
Coordinates   2965826..2966953 (+) Length   375 a.a.
NCBI ID   WP_147072959.1    Uniprot ID   A0A512L884
Organism   Sulfuriferula plumbiphila strain Gro7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2960826..2971953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH91_RS15310 (SFPGR_30380) - 2962069..2962386 (-) 318 WP_147072969.1 DUF167 domain-containing protein -
  GZH91_RS15315 (SFPGR_30390) - 2962399..2962974 (-) 576 WP_147072967.1 YggT family protein -
  GZH91_RS15320 (SFPGR_30400) proC 2963035..2963841 (-) 807 WP_147072965.1 pyrroline-5-carboxylate reductase -
  GZH91_RS15325 (SFPGR_30410) - 2963858..2964541 (-) 684 WP_147072963.1 YggS family pyridoxal phosphate-dependent enzyme -
  GZH91_RS15330 (SFPGR_30420) pilT 2964575..2965618 (+) 1044 WP_147072961.1 type IV pilus twitching motility protein PilT Machinery gene
  GZH91_RS15335 (SFPGR_30430) pilU 2965826..2966953 (+) 1128 WP_147072959.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GZH91_RS15340 (SFPGR_30440) - 2967450..2970230 (+) 2781 WP_147072952.1 EAL domain-containing protein -
  GZH91_RS15345 (SFPGR_30450) - 2970278..2971429 (-) 1152 WP_147072950.1 class I SAM-dependent RNA methyltransferase -
  GZH91_RS15350 (SFPGR_30460) - 2971435..2971842 (-) 408 WP_147072948.1 CopD family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42171.47 Da        Isoelectric Point: 6.5097

>NTDB_id=75276 GZH91_RS15335 WP_147072959.1 2965826..2966953(+) (pilU) [Sulfuriferula plumbiphila strain Gro7]
MDALKALHDLLRLMLSKKASDLFITAGFPPAIKVDGRIAPVSNQTLTPAHTREFARALMNDRQWADFEATHESNFAISPP
EIGRFRVNAFVQQARVGLVLRTITTRIPKFEELNLPPVLKDVAMVKRGLVIFVGGTGSGKSTSLAAMVGFRNENAQDHII
TIEDPVEYVHEHRKSIITQREVGVDTDNWFTALKNTLRQAPDVILIGEVRDRETMEYAIAFAETGHLCLTTLHANSANQA
LDRIINFFPEDRRQQLLMDLSLNLKSFISQRLIARKGTPGRVAAVEILLNSPLISELIFKGEVHEIKEIMSKSRELGMQT
FDQSLFDLYEAGMISYEDALRNADSLNDLRLKIKLQGQESRERDIMSGLDHLDIV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=75276 GZH91_RS15335 WP_147072959.1 2965826..2966953(+) (pilU) [Sulfuriferula plumbiphila strain Gro7]
ATGGACGCCCTCAAAGCCTTGCACGATCTGCTGCGCCTGATGCTCAGCAAAAAAGCCTCGGATTTATTCATCACGGCGGG
CTTTCCTCCCGCCATCAAGGTGGACGGCAGGATCGCGCCGGTATCCAACCAGACCCTCACCCCGGCACACACGCGCGAGT
TTGCCCGCGCGCTGATGAATGATCGTCAGTGGGCAGATTTTGAAGCGACCCATGAGAGCAACTTCGCCATTTCACCGCCG
GAAATCGGCCGCTTTCGCGTCAATGCCTTTGTCCAGCAGGCTCGGGTTGGGCTGGTGTTGCGAACCATCACCACCCGGAT
TCCCAAGTTCGAGGAACTCAACCTGCCGCCGGTGCTCAAGGATGTGGCCATGGTCAAGCGCGGCCTGGTGATTTTTGTCG
GCGGCACCGGCTCGGGCAAATCCACTTCGCTCGCGGCGATGGTCGGCTTCCGCAACGAGAACGCCCAGGATCACATCATT
ACCATCGAAGACCCGGTGGAATACGTCCATGAACACAGGAAATCCATCATCACCCAGCGCGAAGTCGGCGTGGATACCGA
TAACTGGTTTACCGCATTGAAAAACACCCTGCGCCAGGCACCCGACGTAATCCTGATCGGCGAGGTGCGCGACCGGGAAA
CCATGGAATACGCCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGACCACGCTGCACGCCAACAGCGCCAACCAGGCG
CTGGACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCCAGCAGTTGCTGATGGACCTGTCGCTCAACCTCAAGTCGTT
TATCTCGCAGCGCCTGATCGCCCGAAAAGGCACGCCGGGACGCGTCGCCGCAGTCGAGATTCTGCTCAACTCCCCGCTGA
TTTCGGAACTGATTTTCAAAGGCGAGGTTCACGAGATCAAGGAGATCATGTCAAAGTCGCGCGAACTGGGCATGCAGACT
TTCGACCAATCTCTGTTCGACCTCTACGAGGCGGGCATGATCAGCTACGAGGATGCCCTGCGCAACGCCGATTCCCTCAA
CGACCTCAGGCTGAAGATCAAGCTGCAAGGCCAGGAATCCAGAGAGCGCGACATTATGTCGGGACTGGATCACCTGGACA
TCGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A512L884

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.04

94.133

0.584
  pilU Acinetobacter baylyi ADP1

57.66

95.733

0.552
  pilU Vibrio cholerae strain A1552

55.46

92.8

0.515
  pilT Pseudomonas aeruginosa PAK

43.582

89.333

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

89.867

0.389
  pilT Acinetobacter baylyi ADP1

42.136

89.867

0.379
  pilT Acinetobacter baumannii D1279779

42.598

88.267

0.376
  pilT Acinetobacter nosocomialis M2

42.598

88.267

0.376
  pilT Acinetobacter baumannii strain A118

42.598

88.267

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.194

89.333

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

88

0.365
  pilT Vibrio cholerae strain A1552

41.515

88

0.365

Multiple sequence alignment