Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OEG80_RS11185 Genome accession   NZ_CP106752
Coordinates   2645683..2646804 (+) Length   373 a.a.
NCBI ID   WP_133103813.1    Uniprot ID   A0A9E8XFP4
Organism   Psychrobacter sp. SC65A.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2640683..2651804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG80_RS11170 (SC65A3_02254) - 2641872..2643146 (+) 1275 WP_020442650.1 hemolysin family protein -
  OEG80_RS11175 (SC65A3_02255) - 2643373..2644131 (-) 759 WP_263240877.1 YggS family pyridoxal phosphate-dependent enzyme -
  OEG80_RS11180 (SC65A3_02256) pilT 2644471..2645523 (+) 1053 WP_020442648.1 type IV pilus twitching motility protein PilT Machinery gene
  OEG80_RS11185 (SC65A3_02257) pilU 2645683..2646804 (+) 1122 WP_133103813.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OEG80_RS11190 (SC65A3_02258) fur 2646916..2647350 (-) 435 WP_011512663.1 ferric iron uptake transcriptional regulator -
  OEG80_RS11195 (SC65A3_02259) bamE 2647675..2648091 (+) 417 WP_263240878.1 outer membrane protein assembly factor BamE -
  OEG80_RS11200 (SC65A3_02260) - 2648117..2648521 (-) 405 WP_263240879.1 RnfH family protein -
  OEG80_RS11205 (SC65A3_02261) - 2648509..2649651 (-) 1143 WP_263240880.1 hypothetical protein -
  OEG80_RS11210 (SC65A3_02262) - 2649908..2650576 (-) 669 WP_011512659.1 c-type cytochrome -
  OEG80_RS11215 (SC65A3_02263) - 2650919..2651254 (-) 336 WP_263240882.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41688.88 Da        Isoelectric Point: 6.7810

>NTDB_id=734453 OEG80_RS11185 WP_133103813.1 2645683..2646804(+) (pilU) [Psychrobacter sp. SC65A.3]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENNIPTVEELRLPPSLKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=734453 OEG80_RS11185 WP_133103813.1 2645683..2646804(+) (pilU) [Psychrobacter sp. SC65A.3]
ATGGACATTGATAAACTTTTACAATTGATGATTAACAAAAACGGCTCTGACCTTTTTATCACTGCTGATGTCGCCCCTTC
CATGAAAATCAATGGCAAAATACTGCCGGTTGGTAAAACGCCTTTAACTGCTGAGCAGACTATGAGGCTAGTTAAAGGCG
TCATGACAGTCAATCAGCAAAAAGAGTTTGATGAGACCAACGAGTGCCAGTTTGCGATTACTGATCAGGCGCAGCAAGCA
CGTTTTAGGGTCAGTGCCTTTATACAACGTGATATGGCAGGGATGATTTTACGGAAAATCGAAAATAATATCCCTACTGT
CGAGGAGCTACGCCTGCCCCCCTCTCTAAAAGAGCTGGCAATGAAAAAGCGTGGTATTATTTTATTAGTAGGTGCAACCG
GTACGGGTAAATCAACCACCCTTGCGGCAATGATAGGACACCGCAATCAAAACTCGCATGGTCATATTATTACCATAGAA
GATCCTATCGAGTTTGTCCATAAGCATCGTGGCTGTATTATTACCCAGCGCGAAGTCGGTATCGATACCCACTCTTTTGA
AGCCGGTCTAAAAAACACCTTGCGCCAAGCGCCAGATGTGATTTTGATTGGTGAGATTCGCAATCGTGAAGTCATGAGTT
ACGCGATTCAATATGCTGAAACGGGGCATTTGGTCTTTGCAACCTTGCACGCCAATAATGCCAACCAAGCAATTGATCGT
ATTATTCACTTTTTTGAGACCAGTCGCCATAATCAATTGTTTATGGATTTATCGCTTAACTTACAAGCCATTATCGCGCA
GCAGTTGATTCCAACGCCTGACGGTAAGGGACGCCGCGCTGCTATTGAAATTTTATTAAACTCACAGCTGATTAGCGATT
ATATTCGTAAAGGTGAGGTGCACGAAATCAAAGATGTCATGACGCGCTCACGTGATAGCGGTATGCAAACCTTTGACCAA
GCGCTGTTTGATCTATATGAAAACGGTGAGATTACTTATAAAGAAGCGATTCTTCATGCAGACTCTCCCAATGATTTGCG
CTTAAAAATCAAACTCAGCAGCAAAAATGGCGTGGCAAACTTGGATGAAGGCGCGGACAGTTTATCGTTAGATATTACTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.22

100

0.662
  pilU Acinetobacter baylyi ADP1

66.761

95.174

0.635
  pilU Vibrio cholerae strain A1552

52.174

98.66

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.803

98.123

0.41
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

89.812

0.37
  pilT Vibrio cholerae strain A1552

41.194

89.812

0.37
  pilT Acinetobacter baumannii strain A118

39.65

91.957

0.365
  pilT Acinetobacter nosocomialis M2

39.65

91.957

0.365
  pilT Acinetobacter baumannii D1279779

39.65

91.957

0.365
  pilT Legionella pneumophila strain ERS1305867

41.538

87.131

0.362
  pilT Legionella pneumophila strain Lp02

41.538

87.131

0.362