Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OEG80_RS11180 Genome accession   NZ_CP106752
Coordinates   2644471..2645523 (+) Length   350 a.a.
NCBI ID   WP_020442648.1    Uniprot ID   A0A9E8X5L1
Organism   Psychrobacter sp. SC65A.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2639471..2650523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG80_RS11165 (SC65A3_02253) - 2639925..2641628 (+) 1704 WP_263240875.1 NAD+ synthase -
  OEG80_RS11170 (SC65A3_02254) - 2641872..2643146 (+) 1275 WP_020442650.1 hemolysin family protein -
  OEG80_RS11175 (SC65A3_02255) - 2643373..2644131 (-) 759 WP_263240877.1 YggS family pyridoxal phosphate-dependent enzyme -
  OEG80_RS11180 (SC65A3_02256) pilT 2644471..2645523 (+) 1053 WP_020442648.1 type IV pilus twitching motility protein PilT Machinery gene
  OEG80_RS11185 (SC65A3_02257) pilU 2645683..2646804 (+) 1122 WP_133103813.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OEG80_RS11190 (SC65A3_02258) fur 2646916..2647350 (-) 435 WP_011512663.1 ferric iron uptake transcriptional regulator -
  OEG80_RS11195 (SC65A3_02259) bamE 2647675..2648091 (+) 417 WP_263240878.1 outer membrane protein assembly factor BamE -
  OEG80_RS11200 (SC65A3_02260) - 2648117..2648521 (-) 405 WP_263240879.1 RnfH family protein -
  OEG80_RS11205 (SC65A3_02261) - 2648509..2649651 (-) 1143 WP_263240880.1 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39119.98 Da        Isoelectric Point: 6.6713

>NTDB_id=734452 OEG80_RS11180 WP_020442648.1 2644471..2645523(+) (pilT) [Psychrobacter sp. SC65A.3]
MAEQNLSIEDLLRFTVKHKASDLHISAGMPAMIRVDGEMTRINMPEMSHKVVHQLIYDIMNDRQRADFEEFFETDFSFEI
PNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEHI
LTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAK
TIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRIAAHEIMLGTPAIRNLIRENKIAQMYSSIQTGAGDGMIT
LDQTLKNLVARGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=734452 OEG80_RS11180 WP_020442648.1 2644471..2645523(+) (pilT) [Psychrobacter sp. SC65A.3]
ATGGCTGAACAAAATTTAAGCATTGAAGACTTGCTGCGTTTTACTGTCAAGCATAAGGCTTCAGATTTACATATTTCAGC
CGGTATGCCTGCGATGATTCGAGTTGATGGTGAGATGACTCGGATTAATATGCCAGAGATGAGCCATAAGGTAGTTCATC
AGCTCATTTATGACATCATGAATGATAGACAACGTGCCGACTTTGAAGAGTTTTTTGAAACGGATTTTTCTTTTGAGATT
CCAAATCTAGCCCGTTTTCGGGTAAACGCATTTAATCAAAATCGCGGTGCAGGCGCCGTCTTTCGTACGATTCCCTCCAA
AGTTCTAACGATGGAAGATCTAGGTATGGGCGAAGTATTTAAGCGTGTTTCAGACTTTAAACGTGGCGTGGTATTGGTCA
CTGGTCCAACCGGCTCAGGTAAGTCGACCACGCTAGCTGCCATGATTGATTATATCAATGAAACCCGTAAAGAGCATATC
TTGACCATTGAAGACCCAATTGAATTTGTTCATGAGTCCAAAAAGAGTTTGATTAACCAGCGTGAAGTCCATCGCGATAC
CTTGGGGTTCGAGCCAGCATTGCGCTCAGCGTTACGTGAAGATCCGGACGTTATTCTGGTTGGTGAGCTACGAGATCTTG
AGACCATACGCTTAGCGCTCACTGCCGCTGAAACCGGTCACTTAGTTTTCGGCACTTTGCACACCAGTTCCGCTGCCAAA
ACCATTGACCGTGTTATTGACGTCTTCCCTGCTGCTGAAAAAGACATGATTCGAGCGATGCTATCAGAGTCACTACAAGC
CGTTATCTCGCAAACATTGTTACGTCGTCAAACAGGCGGGCGAATTGCCGCCCATGAGATTATGTTAGGTACGCCCGCTA
TTCGTAACTTAATAAGAGAAAATAAAATCGCTCAAATGTACTCTTCTATCCAAACTGGTGCTGGTGATGGCATGATTACT
CTTGATCAAACTCTTAAAAATCTTGTAGCGAGAGGCACAATTAGTAAAGACGTTGCACGACCTTACGCTAAACAGCCTGA
AGCATTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

79.42

98.571

0.783
  pilT Acinetobacter baumannii D1279779

79.42

98.571

0.783
  pilT Acinetobacter nosocomialis M2

79.13

98.571

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.907

98.286

0.766
  pilT Pseudomonas aeruginosa PAK

77.907

98.286

0.766
  pilT Acinetobacter baylyi ADP1

76.163

98.286

0.749
  pilT Legionella pneumophila strain Lp02

72.093

98.286

0.709
  pilT Legionella pneumophila strain ERS1305867

72.093

98.286

0.709
  pilT Neisseria meningitidis 8013

64.928

98.571

0.64
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

94

0.637
  pilT Vibrio cholerae strain A1552

67.781

94

0.637
  pilT Neisseria gonorrhoeae MS11

64.348

98.571

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.445

98.857

0.509
  pilU Acinetobacter baylyi ADP1

41.399

98

0.406
  pilU Vibrio cholerae strain A1552

43.344

92.286

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.003

96.857

0.397