Detailed information    

experimental Experimentally validated

Overview

Name   rexA   Type   Machinery gene
Locus tag   SPR_RS05190 Genome accession   NC_003098
Coordinates   1024754..1028404 (+) Length   1216 a.a.
NCBI ID   WP_000767212.1    Uniprot ID   Q97QP9
Organism   Streptococcus pneumoniae R6     
Function   homologous recombination   
Homologous recombination

Function

In S. pneumoniae, RexAB is required for DNA repair, but not for chromosomal transformation and plasmid establishment.


Genomic Context

Location: 1019754..1033404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS05170 - 1019764..1020210 (-) 447 WP_001820683.1 tyrosine-type recombinase/integrase -
  SPR_RS05175 - 1020110..1020957 (-) 848 Protein_1021 IS630 family transposase -
  SPR_RS05180 - 1021127..1021282 (+) 156 Protein_1022 diacylglycerol kinase family lipid kinase -
  SPR_RS05185 (spr1039) rexB 1021482..1024757 (+) 3276 WP_000772372.1 ATP-dependent nuclease subunit B Machinery gene
  SPR_RS05190 (spr1040) rexA 1024754..1028404 (+) 3651 WP_000767212.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  SPR_RS05195 (spr1041) - 1028574..1029752 (+) 1179 WP_000174757.1 hypothetical protein -

Sequence

Protein


Download         Length: 1216 a.a.        Molecular weight: 140166.85 Da        Isoelectric Point: 5.3964

>NTDB_id=654 SPR_RS05190 WP_000767212.1 1024754..1028404(+) (rexA) [Streptococcus pneumoniae R6]
MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSAQNILVSASAGSGKTFVMAERILDQLARGVEISQLFISTFT
VKAATELKERLEKKISKKIQETDDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLILE
NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWLSESFLKGFEKADFTSEKEKLTE
QIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLILDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADL
KPLADAYNEERKTQFAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADISHYTIEILE
NFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQSIYRFRQADPQIFNEKFQRYAQNPQEGRLII
LKENFRSSSEVLSATNDVFERLMDQEVGEINYDNKHQLVFANTKLTPNPDNKAAFLLYDKDDTGEEEESQTETKLTGEMR
LVIKEILKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLDTLRVIHNPLQDYALVA
LMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMASSQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIW
KIYNDRFYYDYVGALPNGPARQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIHKS
KGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIKLSIPSLTYRQNEEELQLASYSEQM
RLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAKNGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTRE
AIGELETKSPLQDSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGVEIAGQGQSVG
KKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLKQVQTSQAVRDKINLDKILAFFDTVLGQEILAN
TDHLYREQPFSMLKRDQKSQEDFVVRGILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIE
KYLILLGKDEVQVVKV

Nucleotide


Download         Length: 3651 bp        

>NTDB_id=654 SPR_RS05190 WP_000767212.1 1024754..1028404(+) (rexA) [Streptococcus pneumoniae R6]
ATGAAGCTTATTCCCTTTTTAAGTGAGGAGGAGATTCAAAAACTGCAAGAAGCAGAAGCAAATTCGAGCAAGGAACAGAA
GAAAACTGCCGAGCAAATCGAAGCTATCTACACTTCTGCCCAGAATATCCTGGTCTCAGCATCGGCTGGTTCTGGAAAGA
CCTTTGTCATGGCAGAGCGCATTCTGGACCAATTGGCGCGTGGTGTCGAAATTTCTCAACTCTTTATCTCAACCTTTACC
GTCAAGGCTGCAACTGAACTTAAAGAACGTTTAGAGAAAAAAATCAGCAAGAAAATCCAAGAAACAGATGATGTCGACCT
CAAACAACACTTGGGTCGCCAGTTGGCAGACCTACCCAACGCTGCCATTGGAACCATGGATTCTTTCACACAAAAATTCC
TTGGCAAACATGGTTATCTGCTTGATATTGCACCTAATTTCCGTATTTTACAAAACCAAAGCGAGCAACTTATTCTCGAA
AACGAAGTCTTTCATGAGGTCTTTGAAGCGCATTACCAAGGTAAACAGAAAGAGACCTTTAGTCATTTGCTGAAAAACTT
TGCTGGGCGTGGCAAGGACGAACGGGGTCTGCGCCAGCAGGTCTATAAAATCTATGACTTCCTCCAATCCACCAGTAATC
CTCAAAAGTGGCTGAGTGAATCTTTCCTCAAAGGATTTGAGAAAGCTGATTTTACCAGTGAAAAAGAAAAACTGACCGAG
CAAATCAAACAAGCCCTTTGGGATTTGGAAAGCTTTTTCCGTTACCATCTGGATAACGATGCCAAGGAGTTTGCAAAGGC
TGCCTATTTAGAAAATGTTCAGTTAATTCTGGATGAAATTGGCTCCCTAAATCAGGAGTCCGATAGTCAGGCTTATCAGG
CAGTGCTTGCGCGTGTTGTCGCCATCTCTAAGGAGAAAAACGGTCGAGCTCTGACTAATGCCAGCCGTAAGGCTGATTTG
AAGCCCCTGGCTGATGCCTACAACGAAGAGAGAAAGACCCAGTTTGCTAAACTAGGACAATTATCAGACCAGATAGCGAT
TCTCGACTATCAAGAACGTTATCATGGAGACACTTGGAAACTAGCTAAAACCTTCCAATCTTTCATGAGCGATTTTGTAG
AGGCTTATCGTCAGAGAAAACGACAGGAAAATGCCTTTGAATTCGCTGATATCAGCCATTACACCATTGAGATTTTAGAG
AATTTCCCACAAGTTCGTGAGTCTTATCAGGAGCGCTTCCATGAAGTCATGGTCGATGAGTATCAGGATACCAACCATAT
TCAAGAACGGATGCTGGAATTGTTGTCTAATGGCCACAATCGCTTTATGGTGGGAGATATCAAGCAATCCATCTATCGTT
TCCGTCAGGCAGACCCGCAGATTTTCAATGAGAAATTCCAACGCTATGCGCAAAATCCCCAAGAAGGCAGGCTCATTATC
CTCAAGGAAAATTTCCGTAGTAGTTCAGAAGTGCTGTCAGCAACCAATGATGTCTTTGAACGTCTCATGGACCAAGAGGT
CGGCGAAATCAACTATGATAACAAGCACCAGCTTGTTTTTGCCAATACCAAACTGACTCCCAATCCAGACAACAAGGCAG
CATTTCTCCTCTACGACAAGGACGATACAGGTGAGGAAGAAGAGAGTCAAACAGAAACGAAACTAACAGGCGAAATGCGC
TTAGTTATCAAGGAGATTCTGAAACTTCATCAAGAAAAAGGTGTTGCCTTTAAGGAAATTGCCCTTCTGACCTCCAGCCG
CAGTCGTAATGACCAGATTCTCCTCGCCCTGTCTGAGTACGGAATTCCTGTCAAAACTGACGGAGAGCAAAACAATTATC
TCCAATCCCTAGAAGTGCAAGTCATGCTAGACACTCTTCGTGTCATTCACAATCCCCTGCAAGACTACGCCTTGGTTGCC
CTTATGAAGTCTCCAATGTTTGGTTTTGATGAGGATGAGCTAGCACGTTTGTCCCTTCAGAAAGCAGAGGATAAAGTCCA
CGAAAATCTCTATGAGAAACTGGTCAATGCACAAAAAATGGCAAGTAGTCAAAAAGGCTTGATTCACACAGCTCTAGCTG
AAAAACTAAAGCAATTCATGGATATCCTAGCTTCTTGGCGCTTGTATGCCAAAACCCACTCTCTCTATGACTTGATTTGG
AAGATTTACAACGACCGTTTTTATTATGACTATGTTGGGGCTTTGCCGAATGGTCCTGCTAGGCAGGCCAATCTCTATGC
CCTAGCACTGCGTGCTGATCAATTTGAAAAGAGCAATTTCAAAGGTTTGTCGCGTTTTATTCGTATGATTGACCAAGTCT
TAGAAGCCCAGCACGATTTGGCAAGCGTGGCCGTCGCACCGCCAAAAGATGCAGTAGAGCTCATGACCATCCACAAGAGT
AAAGGGCTGGAGTTTCCTTACGTCTTTATCCTCAATATGGATCAAGATTTCAACAAGCAAGACTCTATGTCAGAAGTCAT
TCTCAGTCGTCAGAATGGTCTTGGTGTCAAATATATTGCCAAGATGGAGACAGGGGCAGTAGAAGACCACTATCCTAAAA
CCATCAAACTCTCCATTCCTAGTCTGACCTATAGGCAGAACGAAGAGGAATTACAGCTAGCAAGCTATTCTGAGCAGATG
CGTTTGCTGTATGTTGCTATGACGCGGGCTGAGAAAAAGCTCTATCTTGTCGGCAAGGGTTCTCGTGAAAAGCTGGAATC
CAAGGAATACCCAGCAGCCAAAAATGGGAAACTAAATAGCAATACTAGACTGCAAGCACGGAATTTCCAAGATTGGCTTT
GGGCTATCAGTAAAGTGTTTACTAAGGACAAGCTCAACTTTAGTTATCGTTTTATTGGCGAAGATCAGTTGACCAGAGAA
GCTATCGGAGAGTTGGAAACCAAGAGTCCTCTCCAAGATAGCTCCCAAGCAGACAATCGTCAGTCAGATACCATCAAAGA
AGCTCTGGAAATGCTGAAGGAGGTGGAAGTTTATAATACTCTTCACCGCGCAGCTATTGAACTTCCTAGTGTTCAAACCC
CAAGTCAAATCAAGAAATTCTACGAACCAGTTATGGATATGGAAGGTGTCGAGATTGCTGGTCAAGGTCAGTCAGTAGGC
AAGAAAATCAGCTTCGATTTGCCAGATTTTTCAACCAAAGAAAAGGTAACTGGAGCTGAGATTGGTAGTGCTACTCACGA
ACTCATGCAGAGAATTGACCTCAGCCAGCAACTAACCCTTGCTAGCCTAACAGAAACACTCAAACAAGTTCAAACTAGCC
AAGCTGTCAGAGACAAGATCAATCTTGATAAAATTCTTGCTTTCTTTGACACAGTACTCGGTCAGGAAATTCTTGCTAAT
ACCGACCATCTTTATCGCGAGCAACCTTTCTCCATGCTCAAACGAGACCAAAAGAGTCAGGAAGACTTTGTTGTCCGTGG
TATCCTTGATGGCTATCTGCTTTACGAAAACAAAATTGTTCTGTTCGACTACAAGACAGACCGCTATGATGAACCAAGTC
AACTCGTAGACCGCTATCGTGGTCAGTTAGCTCTATACGAAGAGGCTTTATCACGAGCCTATTCGATTGAAAATATTGAA
AAATACTTGATTTTACTCGGTAAAGACGAGGTTCAAGTTGTAAAAGTATAA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(479-896)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(29-469)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1054-1193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q97QP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] David Halpern et al. (2004) rexAB mutants in Streptococcus pneumoniae. Microbiology (Reading, England) 150(Pt 7):2409-2414. [PMID: 15256582]