Detailed information NTG0000655

Experimentally validated

Overview

• Name: rexB
• Type: Machinery gene
• Locus tag: SPR_RS05185
• Genome accession: NC_003098
• Coordinates: 1021482..1024757 (+)
• Length: 1091 a.a.
• NCBI ID: WP_000772372.1
• Uniprot ID: Q97QQ0
• Organism: Streptococcus pneumoniae R6
• Function: homologous recombination Homologous recombination

Function

In S. pneumoniae, RexAB is required for DNA repair, but not for chromosomal transformation and plasmid establishment.

Genomic Context

Location: 1016482..1029757

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS05155 (spr1034)	-	1016600..1017715 (+)	1116	WP_000706435.1	glycerate kinase	-
SPR_RS05160 (spr1035)	-	1017712..1018158 (-)	447	WP_000131076.1	YueI family protein	-
SPR_RS05165 (spr1036)	eno	1018322..1019626 (+)	1305	WP_000022815.1	surface-displayed alpha-enolase	-
SPR_RS05170	-	1019764..1020210 (-)	447	WP_001820683.1	tyrosine-type recombinase/integrase	-
SPR_RS05175	-	1020110..1020957 (-)	848	Protein_1021	IS630 family transposase	-
SPR_RS05180	-	1021127..1021282 (+)	156	Protein_1022	diacylglycerol kinase family lipid kinase	-
SPR_RS05185 (spr1039)	rexB	1021482..1024757 (+)	3276	WP_000772372.1	ATP-dependent nuclease subunit B	Machinery gene
SPR_RS05190 (spr1040)	rexA	1024754..1028404 (+)	3651	WP_000767212.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
SPR_RS05195 (spr1041)	-	1028574..1029752 (+)	1179	WP_000174757.1	hypothetical protein	-

Sequence

Protein

• Length: 1091 a.a.
• Molecular weight: 124853.65 Da
• Isoelectric Point: 5.7414

>NTDB_id=655 SPR_RS05185 WP_000772372.1 1021482..1024757(+) (rexB) [Streptococcus pneumoniae R6]
MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKERAVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTT
LDDIGLGLAFYKCLAELDPKDLRVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
GQLAQESQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVIGAYASKKAYTSPFSEGNLYQAS
VKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLESSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLH
ENSDLSYKHFRILLGDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLLRTDLYTDL
SQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAPLETLFASRKQKAEKLLQKWSVFLKEGAVTK
QLQDLTTTLEAVEQERQAEVWKAFCHVLEQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLT
ADFVYAIGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSARKQLFLSAPSLFNESE
SKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSLVAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENP
AIPTSPSSKTLAKDTLQALYPAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQLPN
EDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIETIKEEANFGGKDQAFIQLDNGRSV
FVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPHFFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLA
GAVVEASKSMKYQGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYTENGRSIAPYV
QQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREELNR

Nucleotide

• Length: 3276 bp

>NTDB_id=655 SPR_RS05185 WP_000772372.1 1021482..1024757(+) (rexB) [Streptococcus pneumoniae R6]
ATGAAATTACTTTATACTGATATTCGGACTTCTTTGACAGAAATTCTAACAAGAGAGGCAGAAGAGCTAGTTGCAGCTGG
CAAGCGGGTCTTCTACATTGCCCCCAACTCTCTTTCTTTTGAAAAGGAACGCGCCGTGCTGGAATACTTGTCCCAGCAGG
CTTCTTTTTCGATTACCGTCACGCGCTTTGCTCAAATGGCTCGCTATCTGGTCTTGAATGATTTACCAGCTAAAACTACT
CTTGATGATATCGGTCTTGGGTTGGCCTTTTACAAATGCCTTGCCGAACTCGATCCCAAGGACTTGCGTGTTTATGGCGC
TATTAAGCAGGATCCTCAATTGATCCAGCAGTTAATTGAGCTTTACCATGAGATGACCAAATCTCAGATGAGTTTTTTGG
ACTTGGAGAATTTAACAGATGAGGATAAGAGGGCGGATTTACTCTTGATTTTTGAGAAAGTAACAGCCTATCTTAATCAA
GGTCAGTTAGCCCAGGAAAGTCAGTTGTCCCATTTGATTGAGGCTATTGAGAATGACAAGGTAAGTAGTGATTTTAATCA
AATCGCCTTGGTCATTGACGGCTTTACTCGTTTTTCTGCTGAGGAAGAGCGGGTTGTGGACTTACTTCACGGCAAAGGTG
TTGAGATTGTTATCGGGGCTTATGCTAGTAAGAAAGCCTATACCAGTCCTTTTAGCGAGGGCAATCTCTACCAAGCCAGC
GTAAAATTTCTCCATCATCTGGCTTCTAAATACCAAACGCCTGCTCAGGACTGTTCTCAAACTCATGAGAAGATGGATAG
TTTTGACAAGGCCTCTCGTTTGTTGGAGTCTTCTTATGACTTTTCAGAACTCGCTTTGGATGTCGATGAGAAAGACCGTG
AAAATTTACAAATCTGGTCTTGTTTGACGCAAAAGGAGGAGTTGGAGCTAGTAGCCCGTAGTATTCGTCAGAAATTACAT
GAGAACTCAGACCTGAGCTACAAGCATTTTCGTATTCTCTTGGGGGATGTAGCTTCTTACCAGTTATCTCTCAAAACCAT
TTTTGACCAGTATCAGATTCCTTTTTATCTTGGTAGAAGCGAAGCCATGGCTCATCATCCCTTGACTCAGTTTGTCGAGT
CTATTTTAGCTTTAAAACGTTACCGTTTTCGTCAGGAGGATTTGATTAATCTTCTTAGAACTGATTTGTATACTGACCTC
AGTCAGTCTGATATTGATGCTTTTGAGCAATATATCCGCTATCTTGGTATCAATGGCTTGCCAGCCTTTCAGCAAACCTT
CACCAAATCCCACCATGGAAAATTTAATCTTGAGCGTTTGAATGTCCTCCGCCTGAGAATTTTAGCACCTCTTGAAACCC
TCTTTGCCAGCCGAAAACAAAAGGCTGAAAAACTCCTACAAAAATGGAGTGTCTTTCTAAAAGAAGGAGCTGTGACCAAG
CAGTTACAAGATTTGACAACCACTTTGGAAGCTGTAGAACAGGAAAGACAAGCCGAAGTTTGGAAGGCTTTCTGCCATGT
TTTAGAACAATTTGCGACTGTTTTTGCTGGTTCACAGGTTAGTCTGGAAGACTTCCTAGCCTTGCTCCATTCTGGAATGA
GTTTGTCCCAATACCGTACCATTCCAGCAACAGTGGACACTGTTCTGGTGCAGAGTTACGATTTGATTGCACCATTGACT
GCTGACTTTGTCTATGCTATTGGACTAACTCAGGACAATTTACCAAAAATTTCTCAAAACACCAGTCTTCTGACAGATGA
AGAAAGGCAAAACCTAAACCAAGCGACCGAAGAAGGCGTTCAATTACTGATTGCCAGCAGTGAAAATCTCAAGAAAAATC
GCTACACTATGCTTTCCTTGGTCAATTCTGCTCGTAAGCAGTTGTTCTTGTCGGCTCCAAGCCTTTTTAACGAAAGTGAA
AGTAAGGAATCTGCCTATCTTCAAGAGTTGATCCATTTTGGATTTAGGCGGAGAGAGAAGAGGATGAATCACAAAGGACT
GTCTAAGGAGGATATGGGGTCCTATCACAGTCTTTTGTCTAGTCTGGTTGCCTATCACCAGCAGGGTGAGATGAGCGATA
CTGAGCAAGATTTGACTTTTGTCAAGGTTCTGTCGCGTGTCATAGGTAAAAAACTAGATCAGCAAGGTCTGGAAAATCCA
GCTATCCCAACCAGTCCAAGCAGCAAGACCTTAGCCAAGGACACCTTGCAAGCTCTCTATCCTGCCAAACAGGAGTTTTA
CCTGTCTACGTCGGGTTTGACAGAGTTTTATCGCAATGAATACAGTTATTTCCTACGCTACGTTTTAGGCTTGCAGGAGG
AATTACGTTTGCATCCTGATGCCCGTAGTCACGGGAATTTCTTGCATCGTATCTTTGAACGCGCCTTACAGTTGCCTAAT
GAAGATTCCTTTGACCAACGTCTAGAACAAGCTATTCAAGAAACCAGTCAAGAACGCGAATTTGAAGCTATTTATCAAGA
AAGTTTGGAAGCCCAGTTTACCAAGGAAGTTTTGCTTGATGTTGCACGGACAACTGGACATATTCTCCGACACAATCCAG
CCATCGAAACCATCAAAGAAGAAGCAAATTTTGGTGGAAAAGACCAAGCCTTTATTCAATTAGACAATGGACGCAGTGTC
TTTGTACGAGGCAAGGTGGACCGGATTGACCGTTTGAAAGCTAATGGAGCGATAGGAGTAGTAGACTACAAATCCAGTCT
GACTCAGTTCCAGTTTCCTCATTTCTTTAATGGGCTCAATTCTCAGTTACCAACCTATCTTGCTGCCCTAAAAAGAGAAG
GGGAGCAGAACTTTTTCGGCGCCATGTACTTGGAAATGGCTGAACCTGTCCAATCTCTGATGGCGGTAAAAAGTCTGGCA
GGAGCAGTGGTAGAAGCCAGCAAATCTATGAAATACCAAGGGCTCTTCTTGGAAAAAGAAAGCAGTTATTTAGGCGAATT
TTATAACAAAAACAAGGCTAATCAACTGACAGATGAGGAATTTCAGCTCCTACTGGACTACAATGCCTATCTTTACAAGA
AAGCTGCTGAGAAGATTTTAGCAGGCCGGTTCGCCATCAATCCTTATACTGAAAATGGCAGAAGCATTGCCCCATACGTC
CAGCAACATCAGGCTATTACAGGCTTTGAAGCCAATTACCATCTGGGCCAAGCCCGTTTCCTAGAAAAGTTGGACCTAGC
TGATGGCAAGCGTCTGGTCGGAGAAAAACTCAAGCAAGCTTGGCTTGAAAAAATAAGAGAGGAGTTGAATCGATGA

Domains

Predicted by InterProScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (748-1030)

3D structure

• AlphaFold DB: Q97QQ0

References

• [1]: Vanessa Khemici et al. (2021) Tight Interplay between Replication Stress and Competence Induction in Streptococcus pneumoniae. Cells 10(8):1938. [PMID: 34440707]
• [2]: David Halpern et al. (2004) rexAB mutants in Streptococcus pneumoniae. Microbiology (Reading, England) 150(Pt 7):2409-2414. [PMID: 15256582]