Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N5E84_RS14805 Genome accession   NZ_CP104554
Coordinates   3175577..3177262 (+) Length   561 a.a.
NCBI ID   WP_370685857.1    Uniprot ID   -
Organism   Vibrio sp. J502     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3170577..3182262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5E84_RS14785 (N5E84_14785) pilM 3172827..3173837 (+) 1011 WP_019282438.1 type IV pilus assembly protein PilM Machinery gene
  N5E84_RS14790 (N5E84_14790) pilN 3173827..3174429 (+) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  N5E84_RS14795 (N5E84_14795) pilO 3174422..3175015 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  N5E84_RS14800 (N5E84_14800) pilP 3175002..3175523 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  N5E84_RS14805 (N5E84_14805) pilQ 3175577..3177262 (+) 1686 WP_370685857.1 type IV pilus secretin PilQ Machinery gene
  N5E84_RS14810 (N5E84_14810) aroK 3177449..3177970 (+) 522 WP_010317691.1 shikimate kinase AroK -
  N5E84_RS14815 (N5E84_14815) aroB 3177994..3179082 (+) 1089 WP_029388273.1 3-dehydroquinate synthase -
  N5E84_RS14820 (N5E84_14820) - 3179131..3180630 (+) 1500 WP_069211900.1 AAA family ATPase -
  N5E84_RS14825 (N5E84_14825) - 3180700..3181530 (+) 831 WP_019282435.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61142.36 Da        Isoelectric Point: 4.8177

>NTDB_id=730461 N5E84_RS14805 WP_370685857.1 3175577..3177262(+) (pilQ) [Vibrio sp. J502]
MVFRSLLFGLLLSVAGLSVAAETAQTNELKGIDFRVNKNKDAVLIVELASPSTVVNVQKVADGLQIELLKTDVSNEKLYV
LDVKDFATVVESIEVLRKEPSTQLLATIHGEFKYDYSLKGKYLEVVIGKLKEQEKPKPKSILEKEGKLISINFQDIPVRN
VLQLIADYNAFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLEKARLAEELG
DLRSEIIKINFAKASDIAQMIGGEGAVSMLSERGSISIDERTNSLLIRELAENIVVIRQIVESLDIPVKQVQIEARIVTV
NEGNLDELGVRWGFTSTNGNNTAGGSIESNLFQVGKLGESTLPVDDFLNVNLAATSANASSIAFQVAKLGSDMLLDLELS
ALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGDI
VKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSVNNTVDKVPLLGDLPLLGALFRRSYEQVGKSELLIFVTPKVVI
Q

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=730461 N5E84_RS14805 WP_370685857.1 3175577..3177262(+) (pilQ) [Vibrio sp. J502]
ATGGTATTTCGGAGCCTCTTATTTGGCTTATTATTGAGTGTGGCAGGGCTAAGTGTGGCGGCAGAAACGGCGCAGACCAA
CGAGCTTAAAGGCATTGATTTTCGAGTCAATAAAAACAAAGACGCTGTATTGATTGTTGAACTCGCATCACCTTCGACAG
TAGTTAATGTGCAGAAAGTGGCTGACGGTTTACAAATTGAACTGCTTAAGACGGATGTCTCCAATGAGAAACTCTATGTC
TTAGACGTCAAAGATTTTGCAACCGTTGTTGAGAGTATCGAGGTATTGCGCAAAGAGCCAAGTACACAGTTACTGGCGAC
CATTCATGGCGAGTTTAAGTATGATTACAGCTTGAAAGGGAAATACCTTGAGGTGGTGATTGGCAAACTGAAAGAACAAG
AAAAACCTAAGCCGAAGAGCATTCTTGAGAAAGAGGGCAAGCTTATCTCGATTAACTTTCAAGATATTCCTGTGCGAAAT
GTTTTGCAGCTTATCGCAGATTATAACGCGTTTAACCTAGTCGTCTCAGATTCGGTCACTGGTAACCTGACGCTACGGCT
TGATGGCGTGCCTTGGCAGCAAGTGCTCGATATCATTTTGCAAGTCAAGGGATTAGATAAGCGTGTTGACGGCAATGTGA
TTTTAGTTGCACCTAAAGAAGAGTTGGATTTACGTGAAAAGCAGCAGCTTGAAAAGGCACGTTTGGCTGAAGAGTTGGGC
GATCTGCGTTCTGAAATCATCAAAATTAATTTTGCTAAGGCGTCTGATATTGCGCAGATGATTGGCGGTGAAGGGGCGGT
CAGCATGTTGTCTGAGCGAGGTTCCATCAGTATCGACGAGCGAACCAACTCACTGCTGATCCGCGAATTGGCTGAGAATA
TTGTGGTGATACGCCAAATTGTTGAATCGCTCGATATCCCGGTCAAACAGGTACAGATTGAAGCGCGCATTGTTACGGTC
AACGAAGGTAATTTGGATGAACTTGGCGTACGCTGGGGCTTTACTTCGACCAATGGCAACAACACCGCAGGCGGAAGTAT
TGAAAGTAATTTATTCCAAGTGGGCAAGTTGGGGGAAAGCACATTGCCCGTTGATGACTTCCTTAACGTAAACTTAGCGG
CGACCTCCGCTAACGCCTCAAGTATTGCCTTCCAAGTGGCTAAGTTGGGGTCGGATATGTTGTTGGATTTAGAGCTTTCA
GCGCTACAACGTGAGTCGAAAGCGGAGATCATTTCCAGCCCTCGTTTGATCACGACCAACAAGAAACCCGCTTATATCGA
GCAGGGTACGGAGATCCCTTATTTGGAGTCTTCTTCCAGCGGGGCAACGACAGTCGCCTTCAAAAAAGCAGTGCTGAGTT
TAAAAGTGACGCCACAGATAACGCCGGATAATCGTTTAGTGTTAGATCTCAGTGTCACTCAAGACCGGCCTGGGGATATT
GTAAAAACAGGGACGGGAGAAGCGGTTGCAATTAATACTCAGCGTATTGGCACTCAAGTATTGGTCAATAATGGAGAAAC
CGTGGTCCTTGGTGGAATATTCCAACATAGCGTCAACAATACGGTAGATAAGGTGCCATTACTTGGTGATTTGCCATTAC
TTGGTGCATTGTTCCGCCGTTCCTATGAGCAAGTGGGCAAAAGTGAGCTGCTGATTTTCGTGACACCGAAAGTCGTCATT
CAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(401-559)

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

83.542

99.643

0.832
  pilQ Vibrio cholerae strain A1552

83.542

99.643

0.832
  pilQ Vibrio campbellii strain DS40M4

79.129

98.217

0.777
  pilQ Legionella pneumophila strain Lp02

42.222

96.257

0.406
  pilQ Legionella pneumophila strain ERS1305867

42.222

96.257

0.406
  pilQ Pseudomonas aeruginosa PAK

39.855

98.396

0.392
  comQ Acinetobacter baylyi ADP1

39.247

99.465

0.39
  pilQ Acinetobacter baumannii strain A118

36.661

100

0.38
  pilQ Acinetobacter baumannii D1279779

36.661

100

0.38