Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   N5E84_RS14790 Genome accession   NZ_CP104554
Coordinates   3173827..3174429 (+) Length   200 a.a.
NCBI ID   WP_017047271.1    Uniprot ID   A0AAW8GXZ8
Organism   Vibrio sp. J502     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3168827..3179429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5E84_RS14775 (N5E84_14775) oxyR 3169252..3170142 (+) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  N5E84_RS14780 (N5E84_14780) - 3170179..3172677 (-) 2499 WP_019282439.1 penicillin-binding protein 1A -
  N5E84_RS14785 (N5E84_14785) pilM 3172827..3173837 (+) 1011 WP_019282438.1 type IV pilus assembly protein PilM Machinery gene
  N5E84_RS14790 (N5E84_14790) pilN 3173827..3174429 (+) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  N5E84_RS14795 (N5E84_14795) pilO 3174422..3175015 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  N5E84_RS14800 (N5E84_14800) pilP 3175002..3175523 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  N5E84_RS14805 (N5E84_14805) pilQ 3175577..3177262 (+) 1686 WP_370685857.1 type IV pilus secretin PilQ Machinery gene
  N5E84_RS14810 (N5E84_14810) aroK 3177449..3177970 (+) 522 WP_010317691.1 shikimate kinase AroK -
  N5E84_RS14815 (N5E84_14815) aroB 3177994..3179082 (+) 1089 WP_029388273.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 22717.24 Da        Isoelectric Point: 9.6928

>NTDB_id=730458 N5E84_RS14790 WP_017047271.1 3173827..3174429(+) (pilN) [Vibrio sp. J502]
MLHSINLLPWREEQRAAHKRRFMGLVVLGVMSAVGIQWGIGLYFSGQAQQQESRLAYLNQYIQQLDKQINALKMTEQEHK
ALLTRLSVVESLQSQRNKTTELMNLMPQLMPEGVYVDKIKMNGEEIEITGISDSTARLATMLDNFEKSALLSDVNMHSIV
HGNKRFGKEFQTFKVSFMFHSPTPQGSVLTKIRPTGAVHD

Nucleotide


Download         Length: 603 bp        

>NTDB_id=730458 N5E84_RS14790 WP_017047271.1 3173827..3174429(+) (pilN) [Vibrio sp. J502]
ATGTTGCATAGTATTAACTTACTGCCTTGGCGAGAGGAACAAAGAGCGGCGCATAAGCGTCGTTTTATGGGCTTGGTCGT
CTTAGGTGTGATGAGCGCTGTTGGTATTCAATGGGGTATCGGTTTGTATTTCTCTGGGCAAGCGCAGCAGCAAGAGTCTC
GTCTGGCTTATTTGAATCAATACATTCAGCAGCTTGATAAACAGATCAATGCATTAAAAATGACGGAACAAGAACACAAG
GCACTATTAACTCGCTTATCGGTTGTTGAGTCATTGCAAAGCCAACGCAATAAAACCACCGAGTTGATGAACCTGATGCC
ACAGCTTATGCCTGAAGGAGTCTACGTCGATAAGATCAAAATGAATGGTGAAGAAATTGAAATTACGGGCATCAGTGACA
GCACTGCGCGTCTTGCGACCATGCTCGATAATTTTGAAAAATCAGCCTTACTTTCCGATGTCAACATGCACTCTATCGTG
CACGGTAATAAGCGATTTGGTAAGGAGTTTCAGACGTTTAAAGTGTCATTTATGTTTCATTCCCCGACACCGCAGGGCTC
TGTGCTAACCAAGATTCGGCCTACAGGAGCTGTCCATGACTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

67.021

94

0.63
  pilN Vibrio campbellii strain DS40M4

66.129

93

0.615