Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   N5E84_RS14795 Genome accession   NZ_CP104554
Coordinates   3174422..3175015 (+) Length   197 a.a.
NCBI ID   WP_013855887.1    Uniprot ID   A0A233HKC5
Organism   Vibrio sp. J502     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3169422..3180015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5E84_RS14780 (N5E84_14780) - 3170179..3172677 (-) 2499 WP_019282439.1 penicillin-binding protein 1A -
  N5E84_RS14785 (N5E84_14785) pilM 3172827..3173837 (+) 1011 WP_019282438.1 type IV pilus assembly protein PilM Machinery gene
  N5E84_RS14790 (N5E84_14790) pilN 3173827..3174429 (+) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  N5E84_RS14795 (N5E84_14795) pilO 3174422..3175015 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  N5E84_RS14800 (N5E84_14800) pilP 3175002..3175523 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  N5E84_RS14805 (N5E84_14805) pilQ 3175577..3177262 (+) 1686 WP_370685857.1 type IV pilus secretin PilQ Machinery gene
  N5E84_RS14810 (N5E84_14810) aroK 3177449..3177970 (+) 522 WP_010317691.1 shikimate kinase AroK -
  N5E84_RS14815 (N5E84_14815) aroB 3177994..3179082 (+) 1089 WP_029388273.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23039.37 Da        Isoelectric Point: 4.3879

>NTDB_id=730459 N5E84_RS14795 WP_013855887.1 3174422..3175015(+) (pilO) [Vibrio sp. J502]
MTSFQDLDMDEMAEWPLLPQLLVILVLMLIIQGVGAWFYLMPQNEQLQNLKQQEEVLKSTLRIKASKAATLPQLQAQLDE
LTERYDFLLRQLPVQKELASMLASVNELGLDNALTFTRIDWGEKQNKEFLYRLPLNIELTGNYHDIGDFSEAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEKQ

Nucleotide


Download         Length: 594 bp        

>NTDB_id=730459 N5E84_RS14795 WP_013855887.1 3174422..3175015(+) (pilO) [Vibrio sp. J502]
ATGACTAGTTTTCAAGATCTTGATATGGATGAAATGGCTGAGTGGCCGTTGCTGCCTCAACTGCTGGTTATTTTGGTGTT
GATGCTGATTATTCAAGGCGTTGGCGCGTGGTTCTATCTTATGCCTCAAAATGAGCAATTGCAGAACTTAAAACAGCAAG
AAGAGGTGCTGAAATCGACCTTACGGATTAAGGCCAGCAAAGCCGCCACCTTACCCCAGTTGCAAGCTCAACTGGATGAA
TTAACTGAACGTTATGATTTTTTATTACGCCAATTACCCGTGCAAAAAGAGCTCGCAAGCATGCTGGCGTCGGTCAATGA
ACTAGGGCTAGATAACGCGTTAACCTTTACACGTATTGATTGGGGAGAAAAACAGAATAAAGAGTTTCTGTATCGTTTGC
CGCTCAATATCGAATTAACTGGTAATTACCACGACATTGGTGATTTCTCAGAGGCGATCGCGAAATTGCCCCGCATTATT
AATTTCGATGATGTTGATTGGCAACGGGTTAGCCAGGAAAGCAGCACGTTGCATTTTAGGGTGCGCGCTTATACCTATCA
ATTCAAGCAAGAGGTGGACGATGAAAAACAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A233HKC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

80.612

99.492

0.802
  pilO Vibrio campbellii strain DS40M4

70.37

95.939

0.675