Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N5E84_RS14785 Genome accession   NZ_CP104554
Coordinates   3172827..3173837 (+) Length   336 a.a.
NCBI ID   WP_019282438.1    Uniprot ID   A0A289GF81
Organism   Vibrio sp. J502     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3167827..3178837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5E84_RS14770 (N5E84_14770) - 3168377..3169105 (-) 729 WP_013855882.1 redoxin family protein -
  N5E84_RS14775 (N5E84_14775) oxyR 3169252..3170142 (+) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  N5E84_RS14780 (N5E84_14780) - 3170179..3172677 (-) 2499 WP_019282439.1 penicillin-binding protein 1A -
  N5E84_RS14785 (N5E84_14785) pilM 3172827..3173837 (+) 1011 WP_019282438.1 type IV pilus assembly protein PilM Machinery gene
  N5E84_RS14790 (N5E84_14790) pilN 3173827..3174429 (+) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  N5E84_RS14795 (N5E84_14795) pilO 3174422..3175015 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  N5E84_RS14800 (N5E84_14800) pilP 3175002..3175523 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  N5E84_RS14805 (N5E84_14805) pilQ 3175577..3177262 (+) 1686 WP_370685857.1 type IV pilus secretin PilQ Machinery gene
  N5E84_RS14810 (N5E84_14810) aroK 3177449..3177970 (+) 522 WP_010317691.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37427.96 Da        Isoelectric Point: 7.5395

>NTDB_id=730457 N5E84_RS14785 WP_019282438.1 3172827..3173837(+) (pilM) [Vibrio sp. J502]
MGKSLVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSRKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLSIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRQRDKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=730457 N5E84_RS14785 WP_019282438.1 3172827..3173837(+) (pilM) [Vibrio sp. J502]
ATGGGTAAATCATTGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCGCAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAGCATCTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAGATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCGGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGCAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGCGCAGATACTCCGATGTTGGCGCAAGAGATCTCGCGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCAGCGAGATAAGCAGTCTGGGCAGAGTTTTAGCACCGCGGCT
GGCCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A289GF81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.976

100

0.53