Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRS07_RS04900 Genome accession   NZ_CP103371
Coordinates   1096138..1097274 (-) Length   378 a.a.
NCBI ID   WP_259211545.1    Uniprot ID   -
Organism   Massilia sp. H6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1091138..1102274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRS07_RS04885 (NRS07_04885) - 1091165..1091644 (-) 480 WP_259211542.1 glutathione peroxidase -
  NRS07_RS04890 (NRS07_04890) - 1091816..1094065 (+) 2250 WP_259211543.1 TonB-dependent receptor domain-containing protein -
  NRS07_RS04895 (NRS07_04895) - 1094133..1095836 (-) 1704 WP_259211544.1 methyl-accepting chemotaxis protein -
  NRS07_RS04900 (NRS07_04900) pilU 1096138..1097274 (-) 1137 WP_259211545.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NRS07_RS04905 (NRS07_04905) pilT 1097284..1098327 (-) 1044 WP_259211546.1 type IV pilus twitching motility protein PilT Machinery gene
  NRS07_RS04910 (NRS07_04910) - 1098405..1099106 (+) 702 WP_259211547.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRS07_RS04915 (NRS07_04915) proC 1099125..1099940 (+) 816 WP_259211548.1 pyrroline-5-carboxylate reductase -
  NRS07_RS04920 (NRS07_04920) - 1099999..1102203 (-) 2205 WP_307729919.1 S8 family serine peptidase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42009.37 Da        Isoelectric Point: 6.6667

>NTDB_id=722365 NRS07_RS04900 WP_259211545.1 1096138..1097274(-) (pilU) [Massilia sp. H6]
MERDQASKFMFDLLRLMTSKGGSDLFITTGFPPAIKIDGKMTPVSNQPLNGTHTNDLARAIMNDKQAAGFELTKEANFAI
SPGDLGRFRVSAFMQMGSVGMVLRTITTTIPRFEDLELPPVLKDVIMAKRGLVILVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEFVHPHRNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNMKGLVSQRLIPKKEAKGRVVAIEILLNSPLVSDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEADKISYEDALRNADSVNDLRLNIKLNSKHAKNRDFSAGMEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=722365 NRS07_RS04900 WP_259211545.1 1096138..1097274(-) (pilU) [Massilia sp. H6]
ATGGAACGCGACCAGGCATCCAAATTCATGTTCGACCTGCTGCGCCTGATGACCAGCAAGGGCGGCTCGGACCTGTTCAT
CACGACCGGTTTTCCGCCCGCCATCAAGATCGACGGCAAGATGACGCCGGTGTCGAACCAGCCCCTGAACGGCACCCACA
CCAACGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCGGCCGGCTTCGAGCTCACCAAAGAGGCCAACTTCGCGATC
AGCCCTGGCGACCTGGGGCGCTTTCGCGTCTCGGCCTTTATGCAGATGGGCAGCGTAGGCATGGTGCTGCGCACGATCAC
GACCACCATCCCGCGGTTTGAAGACCTCGAGCTGCCGCCAGTACTGAAGGACGTGATCATGGCCAAGCGCGGCCTGGTGA
TCCTGGTCGGCGCCACCGGTTCGGGCAAGTCGACCACGCTGGCCGCGATGGTCGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACGATCGAAGACCCGGTCGAATTCGTGCACCCGCACCGCAACTGCGTGATCACGCAGCGCGAAGTGGGCGT
CGACACCGACAGTTTCGAAGCGGCGTTGAAAAACTCCCTGCGCCAGGCGCCAGACGTGATCCAGATTGGCGAGATCCGCG
ACCGCGAAACCATGGAGCATGCGATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATTAACTTCTTCCCCGAAGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACAT
GAAGGGCCTGGTGTCGCAGCGCCTGATTCCCAAAAAGGAAGCCAAGGGCCGCGTGGTGGCGATCGAAATCCTGCTCAACT
CCCCGCTCGTGTCTGACCTGATCTTCAAGGGCGACGTACACGAGATCAAGGAGATCATGAAAAAGTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTACGAGGCCGACAAGATTTCGTATGAAGACGCGCTGCGCAATGCCGA
TTCGGTCAACGACCTGCGCCTGAACATCAAGCTCAATAGCAAGCACGCGAAGAACCGCGATTTCTCGGCCGGCATGGAGC
ATCTCGGAATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

58.197

96.825

0.563
  pilU Vibrio cholerae strain A1552

56.338

93.915

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.624

0.399
  pilT Pseudomonas aeruginosa PAK

42.388

88.624

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

87.566

0.368
  pilT Legionella pneumophila strain Lp02

41.994

87.566

0.368
  pilT Acinetobacter nosocomialis M2

41.994

87.566

0.368
  pilT Acinetobacter baumannii D1279779

41.994

87.566

0.368
  pilT Acinetobacter baumannii strain A118

41.994

87.566

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.896

88.624

0.362