Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NRG74_RS21770 Genome accession   NZ_CP102428
Coordinates   4701266..4701724 (-) Length   152 a.a.
NCBI ID   WP_307773990.1    Uniprot ID   -
Organism   Pseudomonas oryzihabitans strain YY7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4696266..4706724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG74_RS21750 (NRG74_21750) cyoB 4696492..4698480 (-) 1989 WP_017640825.1 cytochrome o ubiquinol oxidase subunit I -
  NRG74_RS21755 (NRG74_21755) cyoA 4698484..4699407 (-) 924 WP_017640824.1 ubiquinol oxidase subunit II -
  NRG74_RS21765 (NRG74_21765) - 4700180..4701178 (+) 999 WP_257705294.1 SDR family oxidoreductase -
  NRG74_RS21770 (NRG74_21770) pilA/pilAI 4701266..4701724 (-) 459 WP_307773990.1 pilin Machinery gene
  NRG74_RS21780 (NRG74_21780) pilB 4701937..4703631 (+) 1695 WP_257705296.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NRG74_RS21785 (NRG74_21785) pilC 4703844..4705061 (+) 1218 WP_027600839.1 type II secretion system F family protein Machinery gene
  NRG74_RS21790 (NRG74_21790) pilD 4705061..4705933 (+) 873 WP_257705297.1 A24 family peptidase Machinery gene
  NRG74_RS21795 (NRG74_21795) coaE 4705930..4706541 (+) 612 WP_017640819.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15029.04 Da        Isoelectric Point: 8.4653

>NTDB_id=716942 NRG74_RS21770 WP_307773990.1 4701266..4701724(-) (pilA/pilAI) [Pseudomonas oryzihabitans strain YY7]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYQDYVAKAKASAAYADISAGKTGYEYAFVNGSTVDLNATGLKASTGNCSAI
TVAAPSATDGGQADAIVCTIANPGRLGANATIKISRSAATAASGTTAASSGGTYSCVTSNFSDAKFKPTGCS

Nucleotide


Download         Length: 459 bp        

>NTDB_id=716942 NRG74_RS21770 WP_307773990.1 4701266..4701724(-) (pilA/pilAI) [Pseudomonas oryzihabitans strain YY7]
ATGAAAGCTCAAAAAGGCTTCACCTTGATCGAACTGATGATCGTGGTCGCGATCATTGGTATTTTGGCTGCTATCGCAAT
TCCTGCATACCAAGACTATGTAGCAAAGGCTAAAGCATCTGCCGCATATGCTGATATCAGCGCCGGAAAAACCGGATACG
AGTATGCATTTGTGAATGGGAGCACAGTAGATCTTAATGCGACCGGCCTTAAGGCTTCGACTGGCAATTGTTCTGCAATT
ACGGTTGCAGCGCCCAGTGCCACTGATGGCGGTCAGGCCGATGCCATTGTTTGCACCATTGCCAACCCGGGTCGCTTGGG
CGCGAACGCAACGATTAAAATCAGCCGCTCGGCAGCAACAGCTGCATCGGGAACCACCGCCGCGTCTTCCGGCGGCACCT
ATAGTTGCGTTACTAGCAACTTCTCCGACGCAAAATTTAAGCCTACTGGATGCAGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.342

100

0.48
  pilA Pseudomonas aeruginosa PAK

39.264

100

0.421
  pilA Acinetobacter baumannii strain A118

41.558

100

0.421
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.421
  pilA2 Legionella pneumophila str. Paris

41.558

100

0.421
  comP Acinetobacter baylyi ADP1

37.888

100

0.401
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.961

100

0.395
  pilA Vibrio cholerae strain A1552

38.961

100

0.395
  pilA Vibrio cholerae C6706

38.961

100

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.312

100

0.388