Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRG74_RS21780 Genome accession   NZ_CP102428
Coordinates   4701937..4703631 (+) Length   564 a.a.
NCBI ID   WP_257705296.1    Uniprot ID   -
Organism   Pseudomonas oryzihabitans strain YY7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4696937..4708631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG74_RS21755 (NRG74_21755) cyoA 4698484..4699407 (-) 924 WP_017640824.1 ubiquinol oxidase subunit II -
  NRG74_RS21765 (NRG74_21765) - 4700180..4701178 (+) 999 WP_257705294.1 SDR family oxidoreductase -
  NRG74_RS21770 (NRG74_21770) pilA/pilAI 4701266..4701724 (-) 459 WP_307773990.1 pilin Machinery gene
  NRG74_RS21780 (NRG74_21780) pilB 4701937..4703631 (+) 1695 WP_257705296.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NRG74_RS21785 (NRG74_21785) pilC 4703844..4705061 (+) 1218 WP_027600839.1 type II secretion system F family protein Machinery gene
  NRG74_RS21790 (NRG74_21790) pilD 4705061..4705933 (+) 873 WP_257705297.1 A24 family peptidase Machinery gene
  NRG74_RS21795 (NRG74_21795) coaE 4705930..4706541 (+) 612 WP_017640819.1 dephospho-CoA kinase -
  NRG74_RS21800 (NRG74_21800) yacG 4706538..4706735 (+) 198 WP_257705298.1 DNA gyrase inhibitor YacG -
  NRG74_RS21805 (NRG74_21805) - 4706891..4707388 (-) 498 WP_173149049.1 hypothetical protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62453.75 Da        Isoelectric Point: 5.7788

>NTDB_id=716943 NRG74_RS21780 WP_257705296.1 4701937..4703631(+) (pilB) [Pseudomonas oryzihabitans strain YY7]
MNETVMLSGLARQLVDAQLLAEAAAQQAVVKAKQAKVPLVTYLVQQRLVQSRPLAELAAQEFGVPFLDLSAIDKEQLPKD
IVNEKLMQQHHFLPLSKRGNKLFIALSDPTNQQAINDVSFGSKLAVEVVLVEDDKLHQAIERYLDTGTGGLDLGDIDLDL
ETDGSERPQEAVAGTDADDAPVVKFVNKMLLDAIKKGSSDLHFEPYEKMYRVRFRTDGVLHEAARPPVQLVSRISARLKV
MSNMDISERRKPQDGRVKLKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEDAQKELYLQALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTEDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNLATSVNLIIAQRLARKLCNKCKRIHEVPREALLAEGFREEE
IGSFTLYQPIGCEDCNAGYRGRVGIYEVVKVTPALQQMIMEEGNSIEIAARMRKEGFNDLRRSGLVKAMQGITSLEEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=716943 NRG74_RS21780 WP_257705296.1 4701937..4703631(+) (pilB) [Pseudomonas oryzihabitans strain YY7]
ATGAACGAGACGGTGATGTTGTCGGGGCTGGCGCGACAGTTGGTGGATGCTCAGCTGTTGGCGGAGGCGGCAGCGCAACA
GGCGGTGGTCAAGGCCAAGCAGGCCAAGGTGCCGCTGGTGACCTATCTCGTTCAGCAGCGGCTGGTGCAAAGCCGGCCGC
TGGCAGAATTGGCTGCTCAGGAGTTCGGTGTGCCCTTTTTGGATCTATCGGCCATCGACAAGGAGCAACTGCCCAAGGAC
ATCGTCAATGAAAAGCTGATGCAACAGCATCACTTCTTGCCGCTGAGCAAGAGAGGTAACAAGCTTTTCATCGCGCTCTC
GGATCCGACCAACCAGCAGGCCATTAACGATGTGTCCTTTGGCAGCAAGCTGGCGGTCGAGGTCGTGCTGGTCGAGGACG
ACAAGCTCCATCAGGCCATCGAGCGGTATCTGGATACGGGTACGGGTGGTTTGGATCTGGGTGACATTGATCTCGACCTG
GAGACAGATGGCAGCGAGCGTCCTCAAGAAGCCGTAGCCGGCACCGATGCCGACGATGCGCCCGTGGTGAAGTTCGTCAA
CAAGATGCTGCTTGATGCCATCAAGAAGGGCTCGTCCGACCTGCACTTTGAGCCCTATGAAAAGATGTACCGGGTGCGCT
TCCGCACTGACGGCGTCCTGCACGAGGCGGCGCGGCCGCCGGTTCAGCTGGTGTCACGGATATCGGCGCGGCTCAAAGTG
ATGTCCAATATGGATATCTCCGAGCGGCGTAAACCTCAGGATGGGCGGGTCAAGCTCAAGGTCTCCAAGACCAAGTCTAT
CGACTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAGAAGATTGTGATGCGGATTCTGGACTCCTCCAGCGCCCAGA
TGGGGATTGATGCCCTGGGCTACGAGGATGCGCAGAAGGAGCTCTATCTGCAGGCGCTCAAGCAGCCGCAGGGGATGATT
CTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTCTCCCTCTATACCGGCCTCAATATCCTCAATACCGAAGACATCAA
TATTTCCACCGCGGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAATCCTCGTCAGGGACTCG
ACTTCTCCCAGGCGCTACGGGCCTTCCTGCGTCAGGATCCTGACGTGATCATGGTCGGTGAGATTCGGGATCTGGAAACG
GCGGAAATTGCTATCAAGGCAGCCCAGACCGGTCACATGGTGATGTCGACCCTGCACACCAACAGCGCGGCGGAAACCCT
GACCCGCTTGCGTAACATGGGCGTGCCGGCCTTCAACCTGGCGACTTCGGTCAACTTGATCATTGCCCAGCGTCTGGCGC
GCAAGCTGTGCAATAAATGCAAGAGAATCCATGAGGTGCCCCGTGAGGCTCTCTTGGCCGAGGGATTTCGCGAGGAAGAG
ATCGGTAGCTTTACCCTCTACCAACCCATTGGCTGTGAGGACTGCAATGCCGGTTATCGCGGACGCGTGGGTATCTATGA
GGTGGTGAAGGTCACCCCGGCGCTGCAGCAGATGATCATGGAGGAAGGCAACTCCATCGAGATCGCGGCGCGGATGCGGA
AAGAGGGCTTCAATGATCTGCGGCGGTCGGGACTGGTGAAGGCGATGCAGGGGATTACCAGTCTGGAAGAGATCAATCGG
GTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.32

99.645

0.601
  pilB Acinetobacter baylyi ADP1

59.893

99.468

0.596
  pilB Legionella pneumophila strain ERS1305867

54.386

100

0.55
  pilB Vibrio cholerae strain A1552

50

100

0.5
  pilB Vibrio campbellii strain DS40M4

49.556

99.823

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.468

0.491
  pilF Neisseria gonorrhoeae MS11

49.023

99.823

0.489
  pilF Thermus thermophilus HB27

36.964

99.291

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.857

93.085

0.362