Detailed information NTG0708901

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NK667_RS12980
• Genome accession: NZ_CP101125
• Coordinates: 3038526..3039635 (-)
• Length: 369 a.a.
• NCBI ID: WP_054615000.1
• Uniprot ID: -
• Organism: Pseudomonas nunensis strain In5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3033526..3044635

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NK667_RS12975 (NK667_12975)	-	3034544..3038350 (-)	3807	WP_054614999.1	hypothetical protein	-
NK667_RS12980 (NK667_12980)	pilU	3038526..3039635 (-)	1110	WP_054615000.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NK667_RS12985 (NK667_12985)	-	3039767..3042304 (+)	2538	WP_054615001.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
NK667_RS12990 (NK667_12990)	-	3042341..3042646 (+)	306	WP_256207501.1	peptidylprolyl isomerase	-
NK667_RS12995 (NK667_12995)	-	3042761..3043693 (-)	933	WP_054615002.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41370.30 Da
• Isoelectric Point: 6.0977

>NTDB_id=708901 NK667_RS12980 WP_054615000.1 3038526..3039635(-) (pilU) [Pseudomonas nunensis strain In5]
MEIDLLLRQLASQEGSDLYLSTGAPPSARFDGVLKPLSEQRFKPGEIAAIAESIMDAEQRLEFDRELEMNLAISLPGVGR
FRINLFKQRNDVSIVARNIKLDIPRFEDLNLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHSALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVISTLHAHNANQALDRI
INFFPQERREQLLHDLANNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIADLIRRNAFDELKGIMEKSVELGMQTFDGAL
YALVVEGAISEEEALKHADSVNNLRLRLKLHVETLPDQPGSAGEWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=708901 NK667_RS12980 WP_054615000.1 3038526..3039635(-) (pilU) [Pseudomonas nunensis strain In5]
ATGGAAATCGATTTATTGTTGCGGCAACTGGCCAGCCAGGAGGGCTCCGATCTTTACCTCTCCACCGGCGCGCCACCCAG
TGCGCGGTTCGATGGCGTGCTCAAACCCTTGTCCGAACAGCGGTTCAAACCGGGGGAAATCGCGGCCATCGCCGAGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATCTCCCTGCCAGGGGTCGGACGC
TTTCGGATCAACCTGTTCAAACAACGCAATGACGTGTCGATTGTCGCGCGTAACATCAAGCTCGACATCCCGCGCTTCGA
AGACCTCAACCTGCCGCCGGTGCTGCTCGAAACCGTGATGCTCAAGCAAGGCCTGATGCTGTTTGTCGGCGCCACCGACT
CGGGAAAGTCCACCTCCCTGGCGGCGCTGATCGATTACCGTAACCGCCACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAATATATCCATCGGCACAAAAAGTCGATCATTAACCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTTCATTC
CGCGTTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTGCTGATCGGTGAAATCCGCGACCGCGAGACCATGGAGCACG
CGTTGACCTTTGCCGATACCGGTCATCTGGTGATCTCCACCCTGCACGCGCACAACGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCTCAGGAGCGTCGCGAACAATTGCTGCACGACCTGGCCAACAACCTCAAGGCGTTCGTTTCGCAGCG
TCTGGTGCGCACCCGCGACGGCCAGCGCCGGGCGGCGGTGGAGGTGATGTTGGGGACGCCGACCATTGCCGATCTGATCC
GGCGTAATGCGTTTGATGAACTTAAAGGGATCATGGAGAAGTCGGTGGAGTTGGGGATGCAGACATTTGACGGGGCGCTG
TACGCGTTGGTCGTGGAAGGTGCGATCAGTGAGGAGGAGGCGTTAAAACATGCTGATTCAGTGAATAACTTGCGGTTGCG
CCTGAAGTTGCATGTCGAAACGTTACCTGACCAACCAGGCTCTGCGGGAGAGTGGGGGCTGGTGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	96.206	0.545
pilU	Acinetobacter baylyi ADP1	52.958	96.206	0.509
pilU	Vibrio cholerae strain A1552	52	94.851	0.493
pilT	Pseudomonas aeruginosa PAK	40.698	93.225	0.379
pilT	Pseudomonas stutzeri DSM 10701	40.698	93.225	0.379
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363
pilT	Neisseria gonorrhoeae MS11	39.003	92.412	0.36
pilT	Neisseria meningitidis 8013	39.003	92.412	0.36
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36