Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J54_RS03650 Genome accession   NZ_AP018766
Coordinates   728087..728536 (+) Length   149 a.a.
NCBI ID   WP_042593498.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-3 isolate 13H41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723087..733536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J54_RS03630 (CHBNII3_06920) rsmE 723550..724287 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J54_RS03635 (CHBNII3_06930) lnt 724337..725866 (-) 1530 WP_343213337.1 apolipoprotein N-acyltransferase -
  K6J54_RS03640 (CHBNII3_06940) corC 725889..726788 (-) 900 WP_042593501.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J54_RS03645 (CHBNII3_06950) ampD 727421..727972 (-) 552 WP_042593499.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J54_RS03650 (CHBNII3_06960) pilA 728087..728536 (+) 450 WP_042593498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J54_RS03655 (CHBNII3_06970) pilB 728533..729927 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  K6J54_RS03660 (CHBNII3_06980) pilC 729924..731141 (+) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  K6J54_RS03665 (CHBNII3_06990) pilD 731138..731830 (+) 693 WP_014550482.1 A24 family peptidase Machinery gene
  K6J54_RS03670 (CHBNII3_07000) rho 731884..733146 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15464.84 Da        Isoelectric Point: 8.7944

>NTDB_id=70797 K6J54_RS03650 WP_042593498.1 728087..728536(+) (pilA) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQHYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADIKTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=70797 K6J54_RS03650 WP_042593498.1 728087..728536(+) (pilA) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAACATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

93.96

100

0.94
  pilA Haemophilus influenzae Rd KW20

88.591

100

0.886
  pilA Glaesserella parasuis strain SC1401

56.376

100

0.564
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

39.189

99.329

0.389
  pilA Vibrio campbellii strain DS40M4

38.298

94.631

0.362

Multiple sequence alignment