Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K6J54_RS03665 Genome accession   NZ_AP018766
Coordinates   731138..731830 (+) Length   230 a.a.
NCBI ID   WP_014550482.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-3 isolate 13H41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726138..736830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J54_RS03645 (CHBNII3_06950) ampD 727421..727972 (-) 552 WP_042593499.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J54_RS03650 (CHBNII3_06960) pilA 728087..728536 (+) 450 WP_042593498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J54_RS03655 (CHBNII3_06970) pilB 728533..729927 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  K6J54_RS03660 (CHBNII3_06980) pilC 729924..731141 (+) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  K6J54_RS03665 (CHBNII3_06990) pilD 731138..731830 (+) 693 WP_014550482.1 A24 family peptidase Machinery gene
  K6J54_RS03670 (CHBNII3_07000) rho 731884..733146 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J54_RS03675 (CHBNII3_07010) metJ 733394..733711 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J54_RS03680 (CHBNII3_07020) cueR 733725..734111 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J54_RS03685 (CHBNII3_07030) - 734188..734394 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  K6J54_RS03690 (CHBNII3_07040) - 734480..734686 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  K6J54_RS03695 (CHBNII3_07050) - 734760..734966 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26683.67 Da        Isoelectric Point: 8.5626

>NTDB_id=70800 K6J54_RS03665 WP_014550482.1 731138..731830(+) (pilD) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYIELFPQNFLSFQPSVSSIQQKKCGHILRYFLNVGVEFIFLQIAF
KDSTFAIWLGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=70800 K6J54_RS03665 WP_014550482.1 731138..731830(+) (pilD) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
ATGATTTACTTCACAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTTCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGAATTTATATTTTTACAAATTGCCTTC
AAGGACTCTACTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGAGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

89.565

100

0.896
  pilD Haemophilus influenzae Rd KW20

89.13

100

0.891

Multiple sequence alignment