Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   H7678_RS00730 Genome accession   NZ_AP023393
Coordinates   119911..120942 (+) Length   343 a.a.
NCBI ID   WP_050807598.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain 89     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114911..125942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7678_RS00720 (SDSE89_00950) - 118588..118953 (+) 366 WP_003055257.1 DUF1033 family protein -
  H7678_RS00725 (SDSE89_00960) comGA/cglA 119034..119975 (+) 942 WP_003062163.1 competence type IV pilus ATPase ComGA Machinery gene
  H7678_RS00730 (SDSE89_00970) comGB 119911..120942 (+) 1032 WP_050807598.1 competence type IV pilus assembly protein ComGB Machinery gene
  H7678_RS00735 (SDSE89_00980) comGC 120944..121270 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  H7678_RS00740 comGD 121245..121673 (+) 429 WP_037589807.1 competence type IV pilus minor pilin ComGD Machinery gene
  H7678_RS00745 (SDSE89_00990) comGE 121681..121926 (+) 246 WP_003049263.1 competence type IV pilus minor pilin ComGE Machinery gene
  H7678_RS00750 (SDSE89_01000) comGF 121907..122347 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF Machinery gene
  H7678_RS00755 (SDSE89_01010) comGG 122370..122699 (+) 330 WP_003062466.1 competence type IV pilus minor pilin ComGG -
  H7678_RS00760 (SDSE89_01020) comGH 122762..123715 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  H7678_RS00765 (SDSE89_01030) - 123774..124970 (+) 1197 WP_003058485.1 acetate kinase -
  H7678_RS00770 (SDSE89_01040) - 125276..125482 (+) 207 WP_003058508.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38935.31 Da        Isoelectric Point: 9.2055

>NTDB_id=70798 H7678_RS00730 WP_050807598.1 119911..120942(+) (comGB) [Streptococcus dysgalactiae subsp. equisimilis strain 89]
MTFLQRDISVRSKQGLKKLSSKEQYKLIQLLANLLLTGFNLAEIIAFLTKSHLIPEPYVLRMEEALLKGRGLADMLAGIG
FSDAILTQISLADGHGNIETTLVNIQGYLNQMAKLKRKTIEVVTYPIILLVFLFVIILGLRHYVMPQLEAQNQLTIFLTH
FPAIFFGIILFIGVLLGGIWLHWRSQSRLKLFTHFSAYPVLGKLLQQYLTAYYAREWGTLIGQGLELPVILDIMAMEKSL
FMQELAEDIRVSLLAGQAFHDKVATYPFFKKELSLMITYGEIKSKLGSELEIYAQESWTQFFSQLHQATQFIQPIIFLVI
ALTIVMIYAAILLPIYQNMGGVL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=70798 H7678_RS00730 WP_050807598.1 119911..120942(+) (comGB) [Streptococcus dysgalactiae subsp. equisimilis strain 89]
ATGACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAAGGAACAATACAAACT
AATTCAGCTATTAGCTAACCTTTTGTTAACAGGATTTAACTTGGCTGAGATAATAGCTTTCTTAACAAAAAGTCATTTGA
TTCCCGAGCCCTATGTTCTCCGCATGGAAGAAGCACTCTTAAAAGGTCGAGGGCTGGCAGATATGTTAGCTGGGATAGGT
TTTTCGGATGCTATTTTAACTCAAATTAGTTTAGCAGATGGTCACGGAAACATTGAGACGACGCTTGTTAATATTCAGGG
CTATCTCAACCAAATGGCTAAACTTAAACGAAAAACCATTGAAGTCGTCACCTATCCGATTATTCTCCTGGTATTTCTTT
TTGTGATAATATTAGGGTTAAGGCATTATGTTATGCCTCAATTAGAAGCTCAAAATCAGCTTACTATTTTTCTGACTCAT
TTTCCAGCTATTTTCTTTGGTATTATCCTTTTTATAGGTGTGCTGTTAGGGGGAATTTGGCTGCATTGGCGATCGCAAAG
TCGCCTCAAGCTTTTTACTCACTTCAGTGCTTATCCTGTTTTAGGGAAACTCTTACAACAGTATCTGACGGCTTATTATG
CTAGAGAATGGGGAACATTGATTGGACAGGGACTTGAATTACCAGTCATTCTGGATATTATGGCCATGGAGAAATCTTTA
TTCATGCAAGAATTGGCTGAAGATATTCGAGTTAGTTTATTGGCAGGACAAGCTTTTCATGATAAGGTAGCTACCTATCC
TTTCTTTAAAAAAGAGTTAAGTTTGATGATTACTTATGGAGAGATTAAATCCAAGTTAGGAAGTGAACTAGAGATTTATG
CTCAAGAGAGTTGGACTCAGTTTTTCAGCCAACTTCATCAAGCAACTCAGTTTATTCAACCGATTATCTTTTTAGTGATT
GCTCTGACGATTGTCATGATTTATGCAGCAATTTTATTACCAATTTATCAAAATATGGGAGGCGTTTTATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)

Type II secretion system (T2SS), protein F (T2SSF)

(28-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA140

54.545

99.417

0.542
  comGB Streptococcus mutans UA159

53.687

98.834

0.531
  comGB Streptococcus gordonii str. Challis substr. CH1

48.968

98.834

0.484
  comGB/cglB Streptococcus mitis NCTC 12261

46.269

97.668

0.452
  comGB/cglB Streptococcus mitis SK321

45.646

97.085

0.443
  comGB/cglB Streptococcus pneumoniae Rx1

44.144

97.085

0.429
  comGB/cglB Streptococcus pneumoniae D39

44.144

97.085

0.429
  comGB/cglB Streptococcus pneumoniae R6

44.144

97.085

0.429
  comGB/cglB Streptococcus pneumoniae TIGR4

44.144

97.085

0.429
  comGB Lactococcus lactis subsp. cremoris KW2

43.068

98.834

0.426

Multiple sequence alignment