Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6J54_RS03655 Genome accession   NZ_AP018766
Coordinates   728533..729927 (+) Length   464 a.a.
NCBI ID   WP_011271972.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-3 isolate 13H41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723533..734927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J54_RS03630 (CHBNII3_06920) rsmE 723550..724287 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6J54_RS03635 (CHBNII3_06930) lnt 724337..725866 (-) 1530 WP_343213337.1 apolipoprotein N-acyltransferase -
  K6J54_RS03640 (CHBNII3_06940) corC 725889..726788 (-) 900 WP_042593501.1 CNNM family magnesium/cobalt transport protein CorC -
  K6J54_RS03645 (CHBNII3_06950) ampD 727421..727972 (-) 552 WP_042593499.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J54_RS03650 (CHBNII3_06960) pilA 728087..728536 (+) 450 WP_042593498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K6J54_RS03655 (CHBNII3_06970) pilB 728533..729927 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  K6J54_RS03660 (CHBNII3_06980) pilC 729924..731141 (+) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  K6J54_RS03665 (CHBNII3_06990) pilD 731138..731830 (+) 693 WP_014550482.1 A24 family peptidase Machinery gene
  K6J54_RS03670 (CHBNII3_07000) rho 731884..733146 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  K6J54_RS03675 (CHBNII3_07010) metJ 733394..733711 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  K6J54_RS03680 (CHBNII3_07020) cueR 733725..734111 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  K6J54_RS03685 (CHBNII3_07030) - 734188..734394 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  K6J54_RS03690 (CHBNII3_07040) - 734480..734686 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52962.30 Da        Isoelectric Point: 5.6248

>NTDB_id=70798 K6J54_RS03655 WP_011271972.1 728533..729927(+) (pilB) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=70798 K6J54_RS03655 WP_011271972.1 728533..729927(+) (pilB) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

100

100

1
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment