Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NIN90_RS02060 Genome accession   NZ_CP100422
Coordinates   427644..428750 (+) Length   368 a.a.
NCBI ID   WP_238825552.1    Uniprot ID   -
Organism   Vibrio furnissii strain 104486766     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422644..433750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIN90_RS02025 - 422994..423593 (-) 600 WP_254413577.1 XTP/dITP diphosphatase -
  NIN90_RS02030 - 423617..424048 (-) 432 WP_014205827.1 DUF4426 domain-containing protein -
  NIN90_RS02035 yggU 424107..424397 (-) 291 WP_004724263.1 DUF167 family protein YggU -
  NIN90_RS02040 - 424397..424954 (-) 558 WP_004724262.1 YggT family protein -
  NIN90_RS02045 proC 425006..425824 (-) 819 WP_004724261.1 pyrroline-5-carboxylate reductase -
  NIN90_RS02050 - 425862..426569 (-) 708 WP_158108025.1 YggS family pyridoxal phosphate-dependent enzyme -
  NIN90_RS02055 pilT 426595..427632 (+) 1038 WP_004724259.1 type IV pilus twitching motility protein PilT Machinery gene
  NIN90_RS02060 pilU 427644..428750 (+) 1107 WP_238825552.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NIN90_RS02065 ruvX 428847..429272 (-) 426 WP_055467261.1 Holliday junction resolvase RuvX -
  NIN90_RS02070 - 429335..429898 (-) 564 WP_004724256.1 YqgE/AlgH family protein -
  NIN90_RS02075 gshB 429937..430893 (-) 957 WP_004724255.1 glutathione synthase -
  NIN90_RS02080 rsmE 430906..431637 (-) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NIN90_RS02085 - 431745..432446 (-) 702 WP_004724253.1 endonuclease -
  NIN90_RS02090 - 432585..433085 (-) 501 WP_014205820.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41332.36 Da        Isoelectric Point: 6.3422

>NTDB_id=705059 NIN90_RS02060 WP_238825552.1 427644..428750(+) (pilU) [Vibrio furnissii strain 104486766]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAHGDALNEAQVMTLLAAMMDDTRHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPPVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNMHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGELHELKNTMAKSKEVGMQTFDQALY
QLVVEHKISEQDALHSADSANDLRLMLKTQRGDVTSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=705059 NIN90_RS02060 WP_238825552.1 427644..428750(+) (pilU) [Vibrio furnissii strain 104486766]
ATGCAACTTGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCGGATCTATATATCACCGTGGGCGCGCCCGTGTT
GTATCGGGTCGATGGTGAACTGCGCGCCCACGGCGACGCGCTGAATGAAGCGCAGGTCATGACGCTGTTGGCCGCGATGA
TGGATGACACGCGCCATGCCGACTACTTGGCGACGCGTGAAGCCAACTTTGCCATTGTGCGTGATTTAGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTGCCCGGCGCAGTGATCCGCCGCATTGAAACGCAAATTCCCACGTTTGATGAACT
CAAACTGCCACCCGTGTTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGCTGGTGGTGGGGGCGACTGGATCGGGGA
AATCGACCACCATGGCCGCCATGACCGGGTATCGCAATATGCATCGCACCGGGCATATCCTCACCGTTGAAGATCCGATT
GAATTTGTCCATGAACATCAGCGCTGCATCGTGACGCAGCGTGAAGTGGGGTTGGACACGGAAAGTTATGAAGTGGCGCT
GAAGAATTCGCTGCGTCAGGCACCGGACATGATTTTGATCGGCGAGATCCGCAGTCGGGAAACCATGGAATACGCAATGA
CGTTTGCCGAAACGGGGCATTTGTGCATGGCCACTTTGCACGCCAACAACGCCAACCAAGCGCTTGAACGGATTTTACAT
CTGGTGCCGAAAGAGCAGCGTGAACAATTTCTGTTTGATTTGTCGATGAACCTCAAAGGCGTCGTCGGTCAGCAACTGAT
CCGCGACAAAAACGGCCAAGGGCGTCACGGGGTGTTTGAAGTGCTGCTCAACAGCCCGCGGGTGTCGGACATCATTCGTC
GCGGCGAGCTGCATGAGTTGAAAAACACCATGGCGAAATCAAAAGAAGTCGGCATGCAGACGTTTGATCAGGCGCTGTAT
CAGTTGGTGGTTGAGCACAAGATCAGTGAGCAAGATGCGCTGCACAGTGCGGATTCGGCCAATGATCTGCGCTTGATGCT
CAAGACGCAGCGGGGCGATGTGACCAGCAGCGGGTCGCTGGATCATGTGCAAATCGATATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.87

100

0.859
  pilU Pseudomonas stutzeri DSM 10701

56.857

95.109

0.541
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.769

88.315

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361