Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NHZ69_RS23205 Genome accession   NZ_CP099797
Coordinates   5010554..5011003 (-) Length   149 a.a.
NCBI ID   WP_025768757.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PAO1-N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5005554..5016003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHZ69_RS23185 (NHZ69_23185) ampD 5006246..5006812 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NHZ69_RS23190 (NHZ69_23190) - 5006963..5009218 (-) 2256 WP_003112843.1 DUF1631 domain-containing protein -
  NHZ69_RS23195 (NHZ69_23195) nadC 5009504..5010352 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NHZ69_RS23205 (NHZ69_23205) pilA 5010554..5011003 (-) 450 WP_025768757.1 type 4a pilus biogenesis protein PilA Machinery gene
  NHZ69_RS23210 (NHZ69_23210) pilB 5011235..5012935 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NHZ69_RS23215 (NHZ69_23215) pilC 5012939..5014159 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  NHZ69_RS23220 (NHZ69_23220) pilD 5014163..5015035 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  NHZ69_RS23225 (NHZ69_23225) coaE 5015032..5015643 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  NHZ69_RS23230 (NHZ69_23230) yacG 5015640..5015840 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15481.75 Da        Isoelectric Point: 6.2905

>NTDB_id=701601 NHZ69_RS23205 WP_025768757.1 5010554..5011003(-) (pilA) [Pseudomonas aeruginosa strain PAO1-N]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGGKIKIGTTASTATETYV
GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=701601 NHZ69_RS23205 WP_025768757.1 5010554..5011003(-) (pilA) [Pseudomonas aeruginosa strain PAO1-N]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTGGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

67.785

100

0.678
  pilA Acinetobacter baumannii strain A118

48

100

0.483
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.98

100

0.47
  pilA Vibrio cholerae strain A1552

45.946

99.329

0.456
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.946

99.329

0.456
  pilA Vibrio cholerae C6706

45.946

99.329

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  comP Acinetobacter baylyi ADP1

42.484

100

0.436
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.973

0.416
  pilA Acinetobacter nosocomialis M2

44.118

91.275

0.403
  pilA2 Legionella pneumophila str. Paris

38.776

98.658

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.095

98.658

0.376