Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ALSL_RS02115 Genome accession   NZ_AP018560
Coordinates   498247..498687 (-) Length   146 a.a.
NCBI ID   WP_126539974.1    Uniprot ID   A0A2Z6E3I8
Organism   Aerosticca soli strain Dysh456     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 490987..500971 498247..498687 within 0


Gene organization within MGE regions


Location: 490987..500971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALSL_RS02090 (ALSL_0432) - 490987..491835 (+) 849 WP_126536202.1 glycosyltransferase -
  ALSL_RS13555 (ALSL_0433) - 491854..492663 (-) 810 WP_161970918.1 class I SAM-dependent methyltransferase -
  ALSL_RS02100 (ALSL_0434) - 492882..494126 (-) 1245 WP_161970919.1 oligosaccharide flippase family protein -
  ALSL_RS02105 (ALSL_0435) - 494204..496144 (-) 1941 WP_231700274.1 hypothetical protein -
  ALSL_RS02110 (ALSL_0436) - 496141..498183 (-) 2043 WP_126536209.1 tetratricopeptide repeat protein -
  ALSL_RS02115 (ALSL_0437) pilA 498247..498687 (-) 441 WP_126539974.1 pilin Machinery gene
  ALSL_RS02120 (ALSL_0438) - 499000..499440 (-) 441 WP_126536211.1 pilin -
  ALSL_RS02125 (ALSL_0439) - 499649..500971 (-) 1323 WP_161970920.1 glycosyltransferase family 39 protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15199.27 Da        Isoelectric Point: 8.4576

>NTDB_id=70052 ALSL_RS02115 WP_126539974.1 498247..498687(-) (pilA) [Aerosticca soli strain Dysh456]
MKKTVQQGFTLIELMIVVAIIAILAAIAIPAYQDYTIRSQVSEGLSLADGAKTAVAEFYSNTGRLPQSNASAGLATDDSI
GGSYVSKLDVLNSGQIQVTYSGPKVNSKISGGTLNLSPITHSGSTAWTCHGDGTKVTAKYLPSSCR

Nucleotide


Download         Length: 441 bp        

>NTDB_id=70052 ALSL_RS02115 WP_126539974.1 498247..498687(-) (pilA) [Aerosticca soli strain Dysh456]
ATGAAGAAGACTGTCCAACAGGGTTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGCCATCCTGGCGGCGAT
TGCCATTCCCGCCTACCAGGACTACACCATTCGCTCGCAGGTTTCGGAAGGTTTGAGTTTGGCCGATGGTGCCAAGACGG
CCGTGGCCGAGTTCTACAGCAATACCGGCCGCCTCCCCCAGAGCAACGCTTCCGCCGGCCTTGCTACTGATGATAGCATC
GGTGGGAGTTACGTTTCGAAATTGGACGTGTTAAATAGTGGCCAGATCCAGGTCACTTACAGCGGCCCGAAGGTAAATTC
GAAAATCAGTGGGGGCACGCTGAACCTTTCGCCGATCACGCATAGTGGCAGCACGGCTTGGACCTGCCATGGCGACGGTA
CGAAGGTGACCGCGAAGTATCTTCCTTCGTCCTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6E3I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

41.341

100

0.507
  pilE Neisseria gonorrhoeae MS11

46.203

100

0.5
  pilE Neisseria gonorrhoeae strain FA1090

45.86

100

0.493
  pilA2 Legionella pneumophila str. Paris

49.296

97.26

0.479
  pilA2 Legionella pneumophila strain ERS1305867

49.296

97.26

0.479
  comP Acinetobacter baylyi ADP1

42.767

100

0.466
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.759

100

0.445
  pilA/pilA1 Eikenella corrodens VA1

39.49

100

0.425
  pilA Acinetobacter baumannii strain A118

41.781

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.541

100

0.411
  pilA Pseudomonas aeruginosa PAK

35.714

100

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.986

100

0.37

Multiple sequence alignment