Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilAI   Type   Machinery gene
Locus tag   NG828_RS05790 Genome accession   NZ_CP099530
Coordinates   1356115..1356507 (+) Length   130 a.a.
NCBI ID   WP_017910395.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain YT9-19-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1343390..1362790 1356115..1356507 within 0


Gene organization within MGE regions


Location: 1343390..1362790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG828_RS05740 (NG828_05725) - 1343390..1344010 (-) 621 WP_148828482.1 histidine phosphatase family protein -
  NG828_RS05745 (NG828_05730) - 1344226..1346250 (+) 2025 WP_230979387.1 phospholipase C, phosphocholine-specific -
  NG828_RS05750 (NG828_05735) - 1346660..1348969 (-) 2310 WP_267082996.1 TonB-dependent receptor -
  NG828_RS05755 (NG828_05740) rimK 1349245..1350138 (+) 894 WP_026143298.1 30S ribosomal protein S6--L-glutamate ligase -
  NG828_RS05760 (NG828_05745) - 1350402..1350848 (+) 447 WP_223875524.1 hypothetical protein -
  NG828_RS05765 (NG828_05750) - 1350863..1351540 (+) 678 WP_010340533.1 response regulator transcription factor -
  NG828_RS05770 (NG828_05755) - 1351533..1352867 (+) 1335 WP_148828478.1 HAMP domain-containing sensor histidine kinase -
  NG828_RS05775 (NG828_05760) coaE 1352978..1353607 (-) 630 WP_150409182.1 dephospho-CoA kinase -
  NG828_RS05780 (NG828_05765) - 1353621..1354484 (-) 864 WP_017910397.1 A24 family peptidase -
  NG828_RS05785 (NG828_05770) pilC 1354491..1355750 (-) 1260 WP_153751114.1 type II secretion system F family protein Machinery gene
  NG828_RS05790 (NG828_05775) pilA/pilAI 1356115..1356507 (+) 393 WP_017910395.1 pilin Machinery gene
  NG828_RS05800 (NG828_05785) - 1356614..1357039 (+) 426 WP_148828475.1 pilin -
  NG828_RS05805 (NG828_05790) - 1357068..1358906 (+) 1839 WP_153751090.1 hypothetical protein -
  NG828_RS05810 (NG828_05795) - 1358991..1360124 (-) 1134 WP_267082997.1 glycosyltransferase -
  NG828_RS05815 (NG828_05800) - 1360175..1361125 (-) 951 WP_267082998.1 YbhN family protein -
  NG828_RS05820 (NG828_05805) - 1361122..1361910 (-) 789 WP_153751087.1 class I SAM-dependent methyltransferase -
  NG828_RS05825 (NG828_05810) - 1361933..1362790 (-) 858 WP_267082999.1 glycosyltransferase family 2 protein -

Sequence


Protein


Download         Length: 130 a.a.        Molecular weight: 13476.66 Da        Isoelectric Point: 9.5775

>NTDB_id=700415 NG828_RS05790 WP_017910395.1 1356115..1356507(+) (pilA/pilAI) [Xanthomonas sacchari strain YT9-19-2]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEITPGKTQFEVKKNEGSTLSTPAQIGLASSTTRCSI
AVTDTTIACTLKGNAAKIAGKNVTLTRTASTGTWACSSSVDAKYKPTGCS

Nucleotide


Download         Length: 393 bp        

>NTDB_id=700415 NG828_RS05790 WP_017910395.1 1356115..1356507(+) (pilA/pilAI) [Xanthomonas sacchari strain YT9-19-2]
ATGAAGAAGCAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGATGTATCAGGACTACGTCGCCAAGTCGCAGGCCACCGCTGGTCTGGCCGAAATCACCCCGGGCAAGACCCAGTTCG
AGGTGAAGAAGAACGAGGGTAGCACCCTCAGCACGCCGGCCCAGATCGGTTTGGCCTCGAGCACGACCCGTTGCAGCATC
GCCGTGACCGACACCACGATTGCCTGCACCCTGAAGGGCAATGCGGCCAAGATCGCTGGCAAGAACGTTACGCTGACCCG
TACGGCCAGCACCGGTACCTGGGCTTGCAGCTCGTCGGTCGACGCCAAGTACAAGCCGACCGGCTGCAGCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

57.778

100

0.6

  pilA Acinetobacter baumannii strain A118

48.921

100

0.523

  pilA Pseudomonas aeruginosa PAK

44.295

100

0.508

  pilA/pilAII Pseudomonas stutzeri DSM 10701

48.12

100

0.492

  pilA Vibrio cholerae C6706

42.759

100

0.477

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.759

100

0.477

  pilA Vibrio cholerae strain A1552

42.759

100

0.477

  comP Acinetobacter baylyi ADP1

38.776

100

0.438

  pilA2 Legionella pneumophila str. Paris

38.095

100

0.431

  pilA/pilA1 Eikenella corrodens VA1

36.913

100

0.423

  pilA Vibrio parahaemolyticus RIMD 2210633

42.742

95.385

0.408

  pilA2 Legionella pneumophila strain ERS1305867

35.417

100

0.392