Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   M9H69_RS01175 Genome accession   NZ_CP097843
Coordinates   211429..212370 (+) Length   313 a.a.
NCBI ID   WP_213988900.1    Uniprot ID   -
Organism   Streptococcus oralis strain HP01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 206429..217370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9H69_RS01155 (M9H69_01155) tgt 206462..207604 (+) 1143 WP_250315695.1 tRNA guanosine(34) transglycosylase Tgt -
  M9H69_RS01160 (M9H69_01160) - 207736..209553 (+) 1818 WP_250315696.1 acyltransferase family protein -
  M9H69_RS01165 (M9H69_01165) nagA 209694..210845 (+) 1152 WP_084876957.1 N-acetylglucosamine-6-phosphate deacetylase -
  M9H69_RS01170 (M9H69_01170) - 210999..211364 (+) 366 WP_000286396.1 DUF1033 family protein -
  M9H69_RS01175 (M9H69_01175) comGA/cglA/cilD 211429..212370 (+) 942 WP_213988900.1 competence type IV pilus ATPase ComGA Machinery gene
  M9H69_RS01180 (M9H69_01180) comGB/cglB 212318..213334 (+) 1017 WP_213988901.1 competence type IV pilus assembly protein ComGB Machinery gene
  M9H69_RS01185 (M9H69_01185) comGC/cglC 213336..213659 (+) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  M9H69_RS01190 (M9H69_01190) comGD/cglD 213622..214056 (+) 435 WP_250315697.1 competence type IV pilus minor pilin ComGD Machinery gene
  M9H69_RS01195 (M9H69_01195) comGE/cglE 214019..214321 (+) 303 WP_167266658.1 competence type IV pilus minor pilin ComGE Machinery gene
  M9H69_RS01200 (M9H69_01200) comGF/cglF 214284..214745 (+) 462 WP_000250702.1 competence type IV pilus minor pilin ComGF Machinery gene
  M9H69_RS01205 (M9H69_01205) comGG/cglG 214723..215136 (+) 414 WP_195217483.1 competence type IV pilus minor pilin ComGG Machinery gene
  M9H69_RS01210 (M9H69_01210) - 215169..215759 (+) 591 WP_250315698.1 class I SAM-dependent methyltransferase -
  M9H69_RS01215 (M9H69_01215) comYH 215818..216765 (+) 948 WP_250315699.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35638.53 Da        Isoelectric Point: 5.4273

>NTDB_id=692897 M9H69_RS01175 WP_213988900.1 211429..212370(+) (comGA/cglA/cilD) [Streptococcus oralis strain HP01]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKKSSLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFMGQQ
VMSIEDPVEIKQEEMLQLQLNETIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELSVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTNWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=692897 M9H69_RS01175 WP_213988900.1 211429..212370(+) (comGA/cglA/cilD) [Streptococcus oralis strain HP01]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTTATCCCCAAGGA
AAAGTCCTACGAGCTTCACATGCGGGTTGGAGACGAACGCTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTGGCGGGTATGAACGTAGGAGAGAAGAGGCGTAGTCAGCTGGGGTCTTGTGACTATCAG
CATGGGGAGAAGAAGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGACATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACCGGCAACGGGGACTCT
ATCTCTTTGCTGGTCCAGTCGGAAGTGGCAAGACGACTCTGATGCACGAATTAGCCAAGTCTCTGTTTATGGGGCAACAG
GTCATGTCCATCGAAGATCCTGTCGAAATCAAGCAAGAAGAGATGCTCCAGTTACAGTTGAATGAAACGATTGGGCTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCGGATCTCCTGATTATCGGTGAAATTCGGGATAGCGAGACGG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGAGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTATCAGTTGTCCTGCAAGGCGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAGGAACACCAGCCAACTAACTGGAATGAGCAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae D39

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae R6

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

87.54

100

0.875
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.042

0.601
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

44.106

84.026

0.371