Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   STO1_RS08775 Genome accession   NZ_AP018338
Coordinates   1714151..1715092 (-) Length   313 a.a.
NCBI ID   WP_061428665.1    Uniprot ID   A0A224A759
Organism   Streptococcus oralis subsp. tigurinus strain osk_001     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1709151..1720092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STO1_RS08735 (STO1_016630) comYH 1709750..1710703 (-) 954 WP_084917154.1 class I SAM-dependent methyltransferase Machinery gene
  STO1_RS08740 (STO1_016640) - 1710762..1711352 (-) 591 WP_096422845.1 class I SAM-dependent methyltransferase -
  STO1_RS08745 (STO1_016650) comGG/cglG 1711385..1711798 (-) 414 WP_096422847.1 competence type IV pilus minor pilin ComGG Machinery gene
  STO1_RS08750 (STO1_016660) comGF/cglF 1711776..1712237 (-) 462 WP_000250700.1 competence type IV pilus minor pilin ComGF Machinery gene
  STO1_RS08755 (STO1_016670) comGE/cglE 1712200..1712502 (-) 303 WP_000413383.1 competence type IV pilus minor pilin ComGE Machinery gene
  STO1_RS08760 (STO1_016680) comGD/cglD 1712465..1712899 (-) 435 WP_172843654.1 competence type IV pilus minor pilin ComGD Machinery gene
  STO1_RS08765 (STO1_016690) comGC/cglC 1712862..1713185 (-) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  STO1_RS08770 comGB/cglB 1713187..1714203 (-) 1017 WP_162138308.1 competence type IV pilus assembly protein ComGB Machinery gene
  STO1_RS08775 (STO1_016710) comGA/cglA/cilD 1714151..1715092 (-) 942 WP_061428665.1 competence type IV pilus ATPase ComGA Machinery gene
  STO1_RS08780 (STO1_016720) - 1715161..1715526 (-) 366 WP_000286399.1 DUF1033 family protein -
  STO1_RS08785 (STO1_016730) nagA 1715679..1716830 (-) 1152 WP_096422851.1 N-acetylglucosamine-6-phosphate deacetylase -
  STO1_RS08790 (STO1_016740) - 1716971..1718788 (-) 1818 WP_096422853.1 acyltransferase family protein -
  STO1_RS08795 (STO1_016750) tgt 1718922..1720064 (-) 1143 WP_061588011.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35533.42 Da        Isoelectric Point: 5.4273

>NTDB_id=69093 STO1_RS08775 WP_061428665.1 1714151..1715092(-) (comGA/cglA/cilD) [Streptococcus oralis subsp. tigurinus strain osk_001]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQELHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=69093 STO1_RS08775 WP_061428665.1 1714151..1715092(-) (comGA/cglA/cilD) [Streptococcus oralis subsp. tigurinus strain osk_001]
ATGGTACAAGAAATTGCACAGAAAATTATTGCGACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTCATTCCCAAGGA
AAAGTCCTATGAGCTTCACATGCGGGTTGGAGACGAACGATGTCTCGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAATGTAGGGGAGAAGAGACGTAGTCAGCTGGGATCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTGGGAGATTATCGGGGACATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGAGCTGCATTTCTGGTTTCAGGATATGAATGAACTGGGTGAGCAGTACAGGCAACGGGGGCTCT
ATCTCTTTGCAGGTCCAGTCGGCAGTGGTAAGACAACACTGATGCACGAATTAGCCAAGTCTCTCTTTAAGGGGCAGCAG
GTCATGTCCATTGAAGATCCTGTCGAAATCAAGCAGGAAGACATGCTCCAGTTGCAGTTAAATGAGGCGATTGGCTTGAC
CTATGAAAATCTCATCAAACTGTCTCTTCGGCATCGTCCTGATCTCTTGATTATCGGTGAAATTCGTGACAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGAGTTTAT
GAACGCCTTCTGGAGTTGGGGGTGACGGAGGAGGAACTAGCAGTTGTCCTGCAAGGCGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A224A759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.457

100

0.895
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.498

100

0.885
  comYA Streptococcus gordonii str. Challis substr. CH1

78.71

99.042

0.78
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.726

84.026

0.367

Multiple sequence alignment