Detailed information NTG0685466

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: M2I87_RS28140
• Genome accession: NZ_CP096942
• Coordinates: 5835681..5836133 (-)
• Length: 150 a.a.
• NCBI ID: WP_270022565.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NY5524
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5830681..5841133

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I87_RS28105 (M2I87_27980)	-	5831137..5831799 (+)	663	WP_033992423.1	DUF3218 family protein	-
M2I87_RS28110 (M2I87_27985)	fliP	5832082..5832834 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
M2I87_RS28115 (M2I87_27990)	fliQ	5832879..5833148 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
M2I87_RS28120 (M2I87_27995)	fliR	5833148..5833924 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
M2I87_RS28125 (M2I87_28000)	-	5833927..5834187 (+)	261	Protein_5561	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
M2I87_RS28130 (M2I87_28005)	-	5835184..5835426 (+)	243	WP_023082027.1	hypothetical protein	-
M2I87_RS28140 (M2I87_28015)	pilA	5835681..5836133 (-)	453	WP_270022565.1	pilin	Machinery gene
M2I87_RS28145 (M2I87_28020)	pilB	5836365..5838065 (+)	1701	WP_010793793.1	type IV-A pilus assembly ATPase PilB	Machinery gene
M2I87_RS28150 (M2I87_28025)	pilC	5838069..5839290 (+)	1222	Protein_5565	type 4a pilus biogenesis protein PilC	-
M2I87_RS28155 (M2I87_28030)	pilD	5839294..5840166 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
M2I87_RS28160 (M2I87_28035)	coaE	5840163..5840774 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
M2I87_RS28165 (M2I87_28040)	yacG	5840771..5840971 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15529.71 Da
• Isoelectric Point: 6.2775

>NTDB_id=685466 M2I87_RS28140 WP_270022565.1 5835681..5836133(-) (pilA) [Pseudomonas aeruginosa strain NY5524]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTEDTTYV
GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSDKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide

• Length: 453 bp

>NTDB_id=685466 M2I87_RS28140 WP_270022565.1 5835681..5836133(-) (pilA) [Pseudomonas aeruginosa strain NY5524]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGAAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGATAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

• Pilin (bacterial filament) (Pilin) (35-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	67.785	99.333	0.673
pilA/pilAI	Pseudomonas stutzeri DSM 10701	46.667	100	0.467
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.671	100	0.46
pilA	Vibrio cholerae strain A1552	43.671	100	0.46
pilA	Vibrio cholerae C6706	43.671	100	0.46
pilA	Vibrio parahaemolyticus RIMD 2210633	45.578	98	0.447
pilA	Acinetobacter baumannii strain A118	44.295	99.333	0.44
comP	Acinetobacter baylyi ADP1	40.26	100	0.413
pilA2	Legionella pneumophila strain ERS1305867	41.892	98.667	0.413
pilA2	Legionella pneumophila str. Paris	41.216	98.667	0.407
pilA/pilAII	Pseudomonas stutzeri DSM 10701	42.657	95.333	0.407