NTDB

Overview

Name	pilB	Type	Machinery gene
Locus tag	M2I87_RS28145	Genome accession	NZ_CP096942
Coordinates	5836365..5838065 (+)	Length	566 a.a.
NCBI ID	WP_010793793.1	Uniprot ID	A0A367MDN5
Organism	Pseudomonas aeruginosa strain NY5524
Function	power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5831365..5843065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I87_RS28110 (M2I87_27985)	fliP	5832082..5832834 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
M2I87_RS28115 (M2I87_27990)	fliQ	5832879..5833148 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
M2I87_RS28120 (M2I87_27995)	fliR	5833148..5833924 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
M2I87_RS28125 (M2I87_28000)	-	5833927..5834187 (+)	261	Protein_5561	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
M2I87_RS28130 (M2I87_28005)	-	5835184..5835426 (+)	243	WP_023082027.1	hypothetical protein	-
M2I87_RS28140 (M2I87_28015)	pilA	5835681..5836133 (-)	453	WP_270022565.1	pilin	Machinery gene
M2I87_RS28145 (M2I87_28020)	pilB	5836365..5838065 (+)	1701	WP_010793793.1	type IV-A pilus assembly ATPase PilB	Machinery gene
M2I87_RS28150 (M2I87_28025)	pilC	5838069..5839290 (+)	1222	Protein_5565	type 4a pilus biogenesis protein PilC	-
M2I87_RS28155 (M2I87_28030)	pilD	5839294..5840166 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
M2I87_RS28160 (M2I87_28035)	coaE	5840163..5840774 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
M2I87_RS28165 (M2I87_28040)	yacG	5840771..5840971 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
M2I87_RS28170 (M2I87_28045)	-	5841008..5841217 (-)	210	WP_003094660.1	hypothetical protein	-
M2I87_RS28175 (M2I87_28050)	-	5841323..5842012 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
M2I87_RS28180 (M2I87_28055)	-	5842009..5842479 (-)	471	WP_010793791.1	hypothetical protein	-
M2I87_RS28185 (M2I87_28060)	-	5842476..5842901 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 566 a.a. Molecular weight: 62354.24 Da Isoelectric Point: 5.8580

                                >NTDB_id=685467 M2I87_RS28145 WP_010793793.1 5836365..5838065(+) (pilB) [Pseudomonas aeruginosa strain NY5524]

MNNSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD

AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE

GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL

KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG

MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL

ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE

DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV

NRVTKD

Nucleotide

Download Length: 1701 bp

                                >NTDB_id=685467 M2I87_RS28145 WP_010793793.1 5836365..5838065(+) (pilB) [Pseudomonas aeruginosa strain NY5524]

ATGAACAACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA

GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC

TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCTTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC

GCTATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC

CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG

ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG

GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT

CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG

TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC

AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA

GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG

CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC

ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA

CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG

GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG

GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA

GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC

TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA

GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT

TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG

CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC

AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A367MDN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilB	Acinetobacter baumannii D1279779	57.517	100	0.581
pilB	Acinetobacter baylyi ADP1	57.194	99.47	0.569
pilB	Legionella pneumophila strain ERS1305867	55.124	100	0.551
pilB	Vibrio parahaemolyticus RIMD 2210633	50.887	99.647	0.507
pilF	Neisseria gonorrhoeae MS11	50.534	99.293	0.502
pilB	Vibrio cholerae strain A1552	50	99.647	0.498
pilB	Vibrio campbellii strain DS40M4	49.645	99.647	0.495
pilF	Thermus thermophilus HB27	39.572	99.117	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.417	93.11	0.376
pilB/pilB1	Synechocystis sp. PCC 6803	38.764	94.346	0.366

Detailed information NTG0685467