NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	M2J78_RS24435	Genome accession	NZ_CP096912
Coordinates	5245278..5245727 (-)	Length	149 a.a.
NCBI ID	WP_021264133.1	Uniprot ID	A0A509JKL4
Organism	Pseudomonas aeruginosa strain NY7770
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5240278..5250727

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2J78_RS24415 (M2J78_24380)	ampD	5240970..5241536 (-)	567	WP_003104911.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
M2J78_RS24420 (M2J78_24385)	-	5241687..5243942 (-)	2256	WP_016264271.1	DUF1631 domain-containing protein	-
M2J78_RS24425 (M2J78_24390)	nadC	5244228..5245076 (+)	849	WP_021264134.1	carboxylating nicotinate-nucleotide diphosphorylase	-
M2J78_RS24435 (M2J78_24400)	pilA	5245278..5245727 (-)	450	WP_021264133.1	pilin	Machinery gene
M2J78_RS24440 (M2J78_24405)	pilB	5245959..5247659 (+)	1701	WP_021264132.1	type IV-A pilus assembly ATPase PilB	Machinery gene
M2J78_RS24445 (M2J78_24410)	pilC	5247663..5248883 (+)	1221	WP_023101565.1	type 4a pilus biogenesis protein PilC	Machinery gene
M2J78_RS24450 (M2J78_24415)	pilD	5248887..5249759 (+)	873	WP_021264129.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
M2J78_RS24455 (M2J78_24420)	coaE	5249756..5250367 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
M2J78_RS24460 (M2J78_24425)	yacG	5250364..5250564 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 149 a.a. Molecular weight: 15537.82 Da Isoelectric Point: 7.7818

                                >NTDB_id=685086 M2J78_RS24435 WP_021264133.1 5245278..5245727(-) (pilA) [Pseudomonas aeruginosa strain NY7770]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGTNIKIGTDPSTATETYV

GVAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide

Download Length: 450 bp

                                >NTDB_id=685086 M2J78_RS24435 WP_021264133.1 5245278..5245727(-) (pilA) [Pseudomonas aeruginosa strain NY7770]

ATGAAAGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG

AAGAGTCTCTGTCGCGTGGAATTGCTGGTACCAATATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT

GGGGTTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACTGGTGCGGGTGATATTACCTTTAC

CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT

GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A509JKL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	68.667	100	0.691
pilA/pilAI	Pseudomonas stutzeri DSM 10701	46.358	100	0.47
pilA	Acinetobacter baumannii strain A118	47.297	99.329	0.47
comP	Acinetobacter baylyi ADP1	43.59	100	0.456
pilA	Vibrio cholerae C6706	42.949	100	0.45
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.949	100	0.45
pilA	Vibrio cholerae strain A1552	42.949	100	0.45
pilA	Vibrio parahaemolyticus RIMD 2210633	45.578	98.658	0.45
pilA/pilAII	Pseudomonas stutzeri DSM 10701	45.455	95.973	0.436
pilA	Acinetobacter nosocomialis M2	41.429	93.96	0.389
pilA2	Legionella pneumophila str. Paris	39.189	99.329	0.389
pilA2	Legionella pneumophila strain ERS1305867	38.514	99.329	0.383

Detailed information NTG0685086