Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA32_RS24865 Genome accession   NZ_CP109844
Coordinates   5339445..5339909 (-) Length   154 a.a.
NCBI ID   WP_003107295.1    Uniprot ID   Q59794
Organism   Pseudomonas aeruginosa strain PALA32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5334445..5344909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA32_RS24850 (PALA32_04918) nadC 5336966..5337814 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA32_RS24860 (PALA32_04920) - 5337995..5339353 (-) 1359 WP_226342803.1 O-antigen ligase family protein -
  PALA32_RS24865 (PALA32_04921) pilA 5339445..5339909 (-) 465 WP_003107295.1 pilin Machinery gene
  PALA32_RS24870 (PALA32_04922) pilB 5340140..5341840 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA32_RS24875 (PALA32_04923) pilC 5341844..5343061 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PALA32_RS24880 (PALA32_04924) pilD 5343062..5343934 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA32_RS24885 (PALA32_04925) coaE 5343931..5344542 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA32_RS24890 (PALA32_04926) yacG 5344539..5344739 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16316.75 Da        Isoelectric Point: 9.0066

>NTDB_id=680433 PALA32_RS24865 WP_003107295.1 5339445..5339909(-) (pilA) [Pseudomonas aeruginosa strain PALA32]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSTKTPNDTQYDIGFTE
STLLDLKGKEQIKVTDNKNGTVQLVATLGGSSGSAIKGAVITVSRDAQGVWSCNITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=680433 PALA32_RS24865 WP_003107295.1 5339445..5339909(-) (pilA) [Pseudomonas aeruginosa strain PALA32]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCTCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCACCAAGACTCCTAACGATACCCAGTATGACATTGGCTTTACTGAA
TCTACTTTGCTGGATCTCAAGGGTAAAGAGCAGATTAAAGTTACGGATAATAAAAATGGTACCGTGCAGCTAGTTGCTAC
TCTGGGTGGTTCTTCTGGTTCCGCCATCAAAGGAGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAGCTGCA
ATATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q59794

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

43.312

100

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Acinetobacter baumannii strain A118

44.079

98.701

0.435
  comP Acinetobacter baylyi ADP1

38.415

100

0.409
  pilA Ralstonia pseudosolanacearum GMI1000

33.708

100

0.39
  pilA Vibrio parahaemolyticus RIMD 2210633

39.333

97.403

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.419

100

0.377
  pilA Vibrio cholerae strain A1552

36.129

100

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.129

100

0.364
  pilA Vibrio cholerae C6706

36.129

100

0.364
  pilE Neisseria gonorrhoeae MS11

41.791

87.013

0.364