Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MWN52_RS02795 Genome accession   NZ_CP095475
Coordinates   657837..658973 (-) Length   378 a.a.
NCBI ID   WP_130515239.1    Uniprot ID   A0A4Q8LR65
Organism   Pseudoxanthomonas winnipegensis strain ZSY-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 652837..663973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN52_RS02760 (MWN52_02745) coq7 653056..653700 (-) 645 WP_130523740.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  MWN52_RS02765 (MWN52_02750) rplM 653928..654356 (+) 429 WP_130515231.1 50S ribosomal protein L13 -
  MWN52_RS02770 (MWN52_02755) rpsI 654359..654751 (+) 393 WP_130515233.1 30S ribosomal protein S9 -
  MWN52_RS02780 (MWN52_02765) - 655079..655816 (+) 738 WP_286576508.1 2OG-Fe dioxygenase family protein -
  MWN52_RS02785 (MWN52_02770) - 655883..656866 (+) 984 WP_286576509.1 fumarylacetoacetate hydrolase family protein -
  MWN52_RS02790 (MWN52_02775) maiA 656966..657643 (+) 678 WP_130515237.1 maleylacetoacetate isomerase -
  MWN52_RS02795 (MWN52_02780) pilU 657837..658973 (-) 1137 WP_130515239.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MWN52_RS02800 (MWN52_02785) - 659116..659532 (+) 417 WP_286576510.1 DUF4398 domain-containing protein -
  MWN52_RS02805 (MWN52_02790) - 659529..660419 (+) 891 WP_286576511.1 hypothetical protein -
  MWN52_RS02810 (MWN52_02795) - 660753..660971 (+) 219 WP_130515246.1 YdcH family protein -
  MWN52_RS02815 (MWN52_02800) - 661157..662527 (+) 1371 WP_130515247.1 pyridoxal-phosphate dependent enzyme -
  MWN52_RS02820 (MWN52_02805) - 662623..663816 (+) 1194 WP_286576512.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41769.99 Da        Isoelectric Point: 5.9752

>NTDB_id=677111 MWN52_RS02795 WP_130515239.1 657837..658973(-) (pilU) [Pseudoxanthomonas winnipegensis strain ZSY-33]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAVALAEAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELQLPEVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDYRNSSTTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPETAHKNVLMNLALNLRAVVSQRLVKGVDGRRLPAAEVLINTPVIRDLLRRGQIHEIKQAMEESLEDGMESFDQCL
FRMAKEGRISQEEALRAADSRDGLALKFRLSEGASGEHDPYADLYDSSAPSTITHGFG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=677111 MWN52_RS02795 WP_130515239.1 657837..658973(-) (pilU) [Pseudoxanthomonas winnipegensis strain ZSY-33]
ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCCGTCTA
CATCAAGATCGAAGGCAGGCTCTACCCCCTTGGCAACACCGGTCTGCCGCCGGGTATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTCAACATGGCCGTGGCCCTGGCCGAGGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTCATCCGCGCCATCCGCAGCAAGATCCCCTCGATCGA
GGAGCTGCAGCTGCCGGAGGTACTCAAGGACATCATCATGACCCCGCGTGGGCTGGTGCTGCTGGTCGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACTACCGCAACTCCAGCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGCAGTCCATCGTCAATCAGCGTGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGACCACCATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTTTTCCCCGAGACCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCCCAGCG
CCTGGTCAAGGGCGTGGACGGGCGCCGGCTGCCGGCCGCCGAGGTCCTGATCAACACCCCGGTGATCCGCGACCTGCTGC
GCCGCGGCCAGATCCACGAGATCAAGCAGGCCATGGAGGAGTCGCTGGAGGACGGCATGGAGTCCTTCGACCAGTGCCTG
TTCCGCATGGCCAAGGAAGGCAGGATCAGCCAGGAGGAGGCGCTGCGGGCCGCCGATTCGCGCGATGGTCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGCCTCGGGCGAGCACGACCCTTACGCCGATCTGTACGACAGCAGCGCCCCCTCGACGATCA
CGCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q8LR65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.696

94.974

0.481
  pilU Acinetobacter baylyi ADP1

49.3

94.444

0.466
  pilU Vibrio cholerae strain A1552

48.023

93.651

0.45
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.67

100

0.431
  pilT Pseudomonas aeruginosa PAK

41.716

89.418

0.373
  pilT Legionella pneumophila strain ERS1305867

41.42

89.418

0.37
  pilT Legionella pneumophila strain Lp02

41.42

89.418

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.418

0.368
  pilT Acinetobacter baumannii D1279779

40.828

89.418

0.365
  pilT Acinetobacter nosocomialis M2

40.828

89.418

0.365
  pilT Acinetobacter baumannii strain A118

40.828

89.418

0.365
  pilT Acinetobacter baylyi ADP1

40.294

89.947

0.362