Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MUU77_RS15455 Genome accession   NZ_CP095187
Coordinates   3282566..3283702 (+) Length   378 a.a.
NCBI ID   WP_162110521.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. F37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3277566..3288702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU77_RS15430 (MUU77_15430) - 3277847..3279031 (-) 1185 WP_245088608.1 cystathionine gamma-synthase -
  MUU77_RS15435 (MUU77_15435) - 3279131..3280501 (-) 1371 WP_245088610.1 pyridoxal-phosphate dependent enzyme -
  MUU77_RS15440 (MUU77_15440) - 3280616..3280834 (-) 219 WP_245088612.1 YdcH family protein -
  MUU77_RS18515 - 3280876..3281001 (-) 126 WP_256452054.1 hypothetical protein -
  MUU77_RS15445 (MUU77_15445) - 3281191..3282078 (-) 888 WP_245088614.1 OmpA family protein -
  MUU77_RS15450 (MUU77_15450) - 3282075..3282470 (-) 396 WP_245088616.1 DUF4398 domain-containing protein -
  MUU77_RS15455 (MUU77_15455) pilU 3282566..3283702 (+) 1137 WP_162110521.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MUU77_RS15460 (MUU77_15460) maiA 3283752..3284411 (-) 660 WP_245088617.1 maleylacetoacetate isomerase -
  MUU77_RS15465 (MUU77_15465) - 3284510..3285499 (-) 990 WP_245088619.1 fumarylacetoacetate hydrolase family protein -
  MUU77_RS15470 (MUU77_15470) - 3285562..3286299 (-) 738 WP_245088621.1 2OG-Fe dioxygenase family protein -
  MUU77_RS15480 (MUU77_15480) rpsI 3286484..3286876 (-) 393 WP_245088623.1 30S ribosomal protein S9 -
  MUU77_RS15485 (MUU77_15485) rplM 3286879..3287307 (-) 429 WP_245088625.1 50S ribosomal protein L13 -
  MUU77_RS15490 (MUU77_15490) coq7 3287494..3288135 (+) 642 WP_245088627.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  MUU77_RS15495 (MUU77_15495) sugE 3288278..3288592 (-) 315 WP_245088629.1 quaternary ammonium compound efflux SMR transporter SugE -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41781.15 Da        Isoelectric Point: 5.9760

>NTDB_id=676045 MUU77_RS15455 WP_162110521.1 3282566..3283702(+) (pilU) [Pseudoxanthomonas sp. F37]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPVFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELNLPPVLKDIIMTPRGLVLIVGSTGSGKSTSLASMIDYRNTTSTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRLPAAEVLINTPMIRDLLRRGQVHEIKAAMEESLEEGMESFDQCL
FRMVKEGLIEQEEALRAADSRDGLALKFRLSEGSKGEHDPYADYDTGSASPRITHGFG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=676045 MUU77_RS15455 WP_162110521.1 3282566..3283702(+) (pilU) [Pseudoxanthomonas sp. F37]
ATGGACATCGGCTATTTCCTGAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTCCTGACCACGGGGGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCCTTGGGCAATACCGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCGTATTCGC
TGATGGACGAGGGCCAGGTGCCCGTGTTCGAGCGCGAGCTCGAGCTGAACATGGCGATCGCCCTGCAGGACGCCGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTCATCCGCGCCATCCGCAGCGTGATCCCCAGCATCGA
GGAGCTGAACCTGCCGCCGGTGCTGAAGGACATCATCATGACGCCGCGCGGCCTGGTGCTGATCGTGGGGTCCACCGGCT
CGGGCAAGTCGACGTCGCTGGCCTCGATGATCGATTACCGCAACACCACCAGCACCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGCAGTCCATCGTCAACCAGCGCGAAGTGGGCCTGGATACCCACGCCTTCCACAA
TGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTGGACGCCACCACGATGGAAGCGG
CCATCGCTTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCCCTGAACCTGCGCGCGGTGGTCTCGCAGCG
CTTGGTGAAAGGCGTCGACGGGCGCCGCCTGCCCGCCGCCGAGGTGCTGATCAACACACCGATGATCCGCGACCTGCTGC
GCCGCGGGCAGGTGCACGAGATCAAGGCCGCCATGGAGGAATCGCTGGAGGAAGGCATGGAATCCTTCGACCAGTGCCTG
TTCCGGATGGTGAAGGAAGGGCTGATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAGGGCAGCAAGGGCGAGCACGACCCCTACGCCGACTACGACACCGGCAGCGCCTCGCCCCGCATCA
CCCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

94.974

0.492
  pilU Acinetobacter baylyi ADP1

49.153

93.651

0.46
  pilU Vibrio cholerae strain A1552

47.154

97.619

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.297

99.735

0.442
  pilT Pseudomonas aeruginosa PAK

42.012

89.418

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.418

0.368
  pilT Acinetobacter baylyi ADP1

40.588

89.947

0.365
  pilT Acinetobacter baumannii D1279779

40.828

89.418

0.365
  pilT Acinetobacter nosocomialis M2

40.828

89.418

0.365
  pilT Acinetobacter baumannii strain A118

40.828

89.418

0.365
  pilT Legionella pneumophila strain Lp02

40.533

89.418

0.362
  pilT Legionella pneumophila strain ERS1305867

40.533

89.418

0.362