Detailed information NTG0668316

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MON40_RS09570
• Genome accession: NZ_CP094241
• Coordinates: 1989561..1990688 (-)
• Length: 375 a.a.
• NCBI ID: WP_003777544.1
• Uniprot ID: A0A2I1XA04
• Organism: Neisseria macacae ATCC 33926
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1984561..1995688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MON40_RS09555 (MON40_09555)	dnaK	1984911..1986842 (+)	1932	WP_003777552.1	molecular chaperone DnaK	-
MON40_RS09560 (MON40_09560)	alr	1987201..1988259 (-)	1059	WP_003777550.1	alanine racemase	-
MON40_RS09565 (MON40_09565)	gloB	1988534..1989283 (-)	750	WP_003777548.1	hydroxyacylglutathione hydrolase	-
MON40_RS09570 (MON40_09570)	pilU	1989561..1990688 (-)	1128	WP_003777544.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MON40_RS09575 (MON40_09575)	-	1990876..1991577 (+)	702	WP_003777542.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
MON40_RS09580 (MON40_09580)	lepA	1991719..1993512 (+)	1794	WP_039862919.1	translation elongation factor 4	-
MON40_RS09585 (MON40_09585)	lepB	1993512..1994531 (+)	1020	WP_003777539.1	signal peptidase I	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 42172.38 Da
• Isoelectric Point: 6.4185

>NTDB_id=668316 MON40_RS09570 WP_003777544.1 1989561..1990688(-) (pilU) [Neisseria macacae ATCC 33926]
MMTNPPSAKDELFTWLRHMNKYKGSDLFITTNFPPAMKVDGKITPITDEPLTSEKCMEIAFSIMSTKQIEEFSSTNECNF
AISLPDTSRFRVNAMVQRGATALVFRSITSKIPNFDSLNLPPVLKDVVMKKRGLVIFVGGTGSGKSTSLAAMVDHRNENC
QDHIITIEDPIEFVHQHKKSIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCLATLHAN
NSNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLIPREGGKGRVAAVEILLNSPIISEMIQKGEIHSIKEMMKKSTA
MGMQTFDQALYELYERGDISFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide

• Length: 1128 bp

>NTDB_id=668316 MON40_RS09570 WP_003777544.1 1989561..1990688(-) (pilU) [Neisseria macacae ATCC 33926]
ATGATGACCAATCCCCCCAGCGCAAAAGACGAATTGTTCACTTGGCTGCGCCATATGAACAAATACAAAGGCTCCGACCT
CTTTATCACGACCAATTTTCCGCCCGCTATGAAAGTGGACGGCAAAATCACGCCGATTACCGACGAGCCGCTGACCTCCG
AAAAATGTATGGAAATCGCTTTTTCGATTATGTCCACCAAACAAATCGAAGAGTTTTCCTCCACCAACGAATGCAACTTC
GCCATCAGCCTGCCCGACACCAGCCGCTTCCGTGTCAATGCCATGGTGCAGCGTGGTGCAACGGCGTTGGTGTTCCGCTC
CATTACCAGCAAAATCCCGAATTTTGACAGCCTCAATCTGCCACCCGTCTTGAAAGACGTGGTGATGAAAAAACGCGGTC
TTGTGATTTTCGTCGGCGGTACCGGCTCGGGTAAATCCACTTCGCTCGCCGCAATGGTTGACCACCGCAACGAAAATTGC
CAAGACCACATCATCACCATCGAAGACCCGATTGAGTTTGTTCATCAACACAAAAAAAGCATCATTACCCAGCGCGAAGT
CGGCGTGGATACCGAAAACTGGTTTGCCGCGCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATCCTCATCGGCGAGA
TTCGCGACCGCGAAACCATGGACTACGCCTTGGCGTTCGCCGAAACAGGCCACCTCTGTCTCGCCACGCTGCACGCCAAC
AACTCCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCACCCAGCTTTTGACCGACTTGTCGCT
CAACCTGCAAGCCTTCATCTCGCAACGCCTGATTCCCCGTGAAGGCGGCAAGGGGCGCGTAGCCGCAGTGGAAATCCTGC
TCAATTCGCCGATTATTTCCGAAATGATTCAAAAAGGCGAAATCCACAGCATCAAAGAAATGATGAAAAAATCTACCGCC
ATGGGCATGCAAACCTTCGACCAAGCGCTCTACGAGCTGTACGAACGCGGCGACATCAGTTTCCAAGACGCCATTAAAAA
CGCCGACTCCGCCCACGACCTGCGTTTGGACATCCAACTGCGCAGCCGCCGCGCCCAAAACGCCGGCCCGGACTTGGAAC
TGATTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (57-257)

3D structure

• AlphaFold DB: A0A2I1XA04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.523	93.867	0.549
pilU	Acinetobacter baylyi ADP1	58.017	91.467	0.531
pilU	Vibrio cholerae strain A1552	52.368	95.733	0.501
pilT	Acinetobacter nosocomialis M2	43.519	86.4	0.376
pilT	Acinetobacter baumannii D1279779	43.519	86.4	0.376
pilT	Acinetobacter baumannii strain A118	43.519	86.4	0.376
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.342	88.8	0.376
pilT	Legionella pneumophila strain Lp02	43.21	86.4	0.373
pilT	Legionella pneumophila strain ERS1305867	43.21	86.4	0.373
pilT	Pseudomonas aeruginosa PAK	42.073	87.467	0.368
pilT	Acinetobacter baylyi ADP1	41.768	87.467	0.365
pilT	Neisseria meningitidis 8013	40.299	89.333	0.36