Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MNQ95_RS09630 Genome accession   NZ_CP093331
Coordinates   2126873..2128009 (-) Length   378 a.a.
NCBI ID   WP_162408431.1    Uniprot ID   -
Organism   Pseudoxanthomonas daejeonensis strain S9-A64R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2121873..2133009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNQ95_RS09605 (MNQ95_09605) - 2122246..2122635 (-) 390 WP_241952723.1 phasin family protein -
  MNQ95_RS09610 (MNQ95_09610) queD 2122861..2123214 (-) 354 WP_241952724.1 6-carboxytetrahydropterin synthase QueD -
  MNQ95_RS09615 (MNQ95_09615) - 2123298..2124668 (-) 1371 WP_241952725.1 DEAD/DEAH box helicase -
  MNQ95_RS09620 (MNQ95_09620) - 2125057..2126055 (+) 999 WP_162408433.1 fumarylacetoacetate hydrolase family protein -
  MNQ95_RS09625 (MNQ95_09625) maiA 2126150..2126833 (+) 684 WP_162408432.1 maleylacetoacetate isomerase -
  MNQ95_RS09630 (MNQ95_09630) pilU 2126873..2128009 (-) 1137 WP_162408431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNQ95_RS09635 (MNQ95_09635) - 2128132..2128527 (+) 396 WP_162408541.1 DUF4398 domain-containing protein -
  MNQ95_RS09640 (MNQ95_09640) - 2128524..2129405 (+) 882 WP_241952726.1 hypothetical protein -
  MNQ95_RS09645 (MNQ95_09645) - 2129816..2130031 (+) 216 WP_162408540.1 YdcH family protein -
  MNQ95_RS09650 (MNQ95_09650) - 2130338..2131714 (+) 1377 WP_241952727.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41822.30 Da        Isoelectric Point: 6.2370

>NTDB_id=663675 MNQ95_RS09630 WP_162408431.1 2126873..2128009(-) (pilU) [Pseudoxanthomonas daejeonensis strain S9-A64R]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEDLNLPDVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDYRNSNTTGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGLDERRIPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMHTFDQCL
FRMVKQGRIEQEEALRAADSRDGLALKFRLSEGADAEHDPYADVNAIPATPTITHGFT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=663675 MNQ95_RS09630 WP_162408431.1 2126873..2128009(-) (pilU) [Pseudoxanthomonas daejeonensis strain S9-A64R]
ATGGATATCGGCTACTTTCTGAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTCCTGACCACCGGGGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTCTACCCGCTCGGCAACACCGGGCTGCCCCCGGGCATGGTCAAGAAGATCGCGTATTCGC
TGATGGACGAGGGCCAGGTGCCACAGTTCGAGCGGGAGCTCGAGCTCAACATGGCGATCGCCCTGGCCGACGCCGGCCGC
TTCCGGGTCAACGTCTTCAAGCAGCGCGGCGAGGTAGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCCAGCATCGA
GGACCTCAACCTCCCAGACGTGCTGAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGTTGGTCGGTTCCACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACTACCGCAACAGCAACACGACCGGGCATATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACCACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCATCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTTTCGCTGCG
CCTGGTCAAGGGCCTGGACGAGCGCCGGATCCCGGCCACCGAGGTACTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGGGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGTTCGCTGGAAGAAGGCATGCATACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGCAGGGCCGCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTCAA
GTTCCGCCTGTCCGAGGGTGCCGATGCCGAGCACGACCCCTACGCCGACGTGAACGCGATACCGGCCACGCCGACCATCA
CCCACGGCTTCACCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

94.974

0.497
  pilU Acinetobacter baylyi ADP1

48.459

94.444

0.458
  pilU Vibrio cholerae strain A1552

47.472

94.18

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.767

91.005

0.407
  pilT Acinetobacter baumannii strain A118

41.42

89.418

0.37
  pilT Acinetobacter nosocomialis M2

41.42

89.418

0.37
  pilT Acinetobacter baumannii D1279779

41.42

89.418

0.37
  pilT Pseudomonas aeruginosa PAK

41.124

89.418

0.368
  pilT Legionella pneumophila strain Lp02

41.124

89.418

0.368
  pilT Legionella pneumophila strain ERS1305867

41.124

89.418

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.533

89.418

0.362
  pilT Acinetobacter baylyi ADP1

40.294

89.947

0.362