Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MJ904_RS11535 Genome accession   NZ_CP092780
Coordinates   2529355..2530491 (-) Length   378 a.a.
NCBI ID   WP_240737493.1    Uniprot ID   -
Organism   Massilia sp. MB5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2524355..2535491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ904_RS11520 (MJ904_11520) glcF 2525005..2526243 (+) 1239 WP_240737491.1 glycolate oxidase subunit GlcF -
  MJ904_RS11525 (MJ904_11525) - 2526230..2528692 (-) 2463 WP_240737492.1 bifunctional diguanylate cyclase/phosphodiesterase -
  MJ904_RS11530 (MJ904_11530) - 2528870..2529355 (-) 486 WP_050409381.1 glutathione peroxidase -
  MJ904_RS11535 (MJ904_11535) pilU 2529355..2530491 (-) 1137 WP_240737493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJ904_RS11540 (MJ904_11540) pilT 2530519..2531562 (-) 1044 WP_050409379.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ904_RS11545 (MJ904_11545) - 2531638..2532366 (+) 729 WP_240737494.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJ904_RS11550 (MJ904_11550) proC 2532363..2533200 (+) 838 Protein_2280 pyrroline-5-carboxylate reductase -
  MJ904_RS11555 (MJ904_11555) - 2533365..2534180 (+) 816 WP_240737495.1 transporter substrate-binding domain-containing protein -
  MJ904_RS11560 (MJ904_11560) - 2534265..2534636 (-) 372 WP_082219531.1 YqjD family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41891.38 Da        Isoelectric Point: 6.7284

>NTDB_id=660279 MJ904_RS11535 WP_240737493.1 2529355..2530491(-) (pilU) [Massilia sp. MB5]
MERDQASKFMFDLLRLMVSKKGSDLFITAGFPPAIKIDGKLTPVSNQVLTPAHTADLARSIMNDKQTASFEQTKEANFAI
SPGDLGRFRVSAFVQMSSVGMVLRTINSAIPKLEDLGLPEVLKEVIMAKRGLVIMVGATGSGKSTTLAAMVGYRNENSHG
HIITIEDPVEFVHPHKNCIITQREVGVDTEDWEVALKNTLRQAPDVIQIGEIRDRQTMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPLKEAKGRAVAIEIMLNSPLISDLIFKGQVHEIKELMKKSRELG
MQTFDQSLFDLHEADKISYEDALRNADSVNDLRLTIKLEGKAAKTRDLSAGTEHLGII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=660279 MJ904_RS11535 WP_240737493.1 2529355..2530491(-) (pilU) [Massilia sp. MB5]
ATGGAACGCGACCAGGCATCCAAATTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAAAAAGGATCGGACCTGTTCAT
CACGGCCGGCTTCCCGCCCGCCATCAAGATCGACGGCAAGCTCACGCCGGTGTCCAACCAGGTGCTGACCCCGGCCCACA
CGGCCGACCTGGCGCGCTCGATCATGAACGACAAGCAGACCGCCAGCTTCGAGCAGACCAAGGAAGCCAATTTCGCCATC
AGCCCCGGCGACCTGGGACGCTTCCGCGTTTCGGCCTTTGTGCAGATGAGTTCCGTCGGCATGGTGCTGCGCACGATCAA
CAGCGCCATCCCCAAGCTGGAAGACCTGGGCCTGCCCGAGGTGCTGAAGGAGGTCATCATGGCCAAGCGCGGCCTGGTGA
TCATGGTGGGCGCCACCGGTTCCGGCAAATCGACCACGCTGGCGGCCATGGTGGGCTACCGCAACGAGAACAGCCACGGC
CACATCATCACCATCGAAGACCCGGTGGAATTCGTCCATCCGCACAAGAACTGCATCATCACCCAGCGCGAAGTGGGCGT
GGATACCGAGGACTGGGAAGTGGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCCAGACCATGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAACGGCGCCAGCAACTGCTGATGGACCTGTCGCTCAACCT
GAAAGGCATGATCTCGCAGCGCCTGATTCCGCTCAAGGAAGCCAAGGGCCGCGCGGTCGCCATCGAGATCATGCTCAATT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCCAGGTGCATGAGATCAAGGAGCTGATGAAAAAATCGCGCGAGCTGGGC
ATGCAGACTTTCGACCAGTCCCTGTTCGACCTGCACGAAGCCGACAAGATCAGCTACGAGGATGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGACCATCAAGCTGGAAGGCAAGGCCGCCAAGACGCGCGACCTGTCGGCCGGCACCGAAC
ACCTGGGGATCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.162

91.534

0.587
  pilU Acinetobacter baylyi ADP1

59.384

94.444

0.561
  pilU Vibrio cholerae strain A1552

58.944

90.212

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.985

88.624

0.381
  pilT Pseudomonas aeruginosa PAK

42.985

88.624

0.381
  pilT Acinetobacter nosocomialis M2

42.9

87.566

0.376
  pilT Legionella pneumophila strain ERS1305867

42.296

87.566

0.37
  pilT Legionella pneumophila strain Lp02

42.296

87.566

0.37
  pilT Acinetobacter baylyi ADP1

43.438

84.656

0.368
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Acinetobacter baumannii D1279779

43.533

83.862

0.365
  pilT Acinetobacter baumannii strain A118

43.533

83.862

0.365