Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   STRIC_RS08520 Genome accession   NZ_AEUX02000007
Coordinates   165492..166436 (+) Length   314 a.a.
NCBI ID   WP_008089649.1    Uniprot ID   G5K4K7
Organism   Streptococcus ictaluri 707-05     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 160492..171436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STRIC_RS08510 (STRIC_1742) rpoC 161280..164918 (+) 3639 WP_040832320.1 DNA-directed RNA polymerase subunit beta' -
  STRIC_RS08515 (STRIC_1743) - 165045..165410 (+) 366 WP_008090041.1 DUF1033 family protein -
  STRIC_RS08520 (STRIC_1744) comYA 165492..166436 (+) 945 WP_008089649.1 competence type IV pilus ATPase ComGA Machinery gene
  STRIC_RS08525 (STRIC_1745) comYB 166369..167403 (+) 1035 WP_050897398.1 competence type IV pilus assembly protein ComGB Machinery gene
  STRIC_RS08530 (STRIC_1746) comYC 167404..167730 (+) 327 WP_008090323.1 competence type IV pilus major pilin ComGC Machinery gene
  STRIC_RS08535 (STRIC_1747) comGD 167705..168133 (+) 429 WP_008090412.1 competence type IV pilus minor pilin ComGD -
  STRIC_RS08540 (STRIC_1748) comGE 168141..168380 (+) 240 WP_234944206.1 competence type IV pilus minor pilin ComGE -
  STRIC_RS08545 comYF 168340..168801 (+) 462 WP_050897399.1 competence type IV pilus minor pilin ComGF Machinery gene
  STRIC_RS08550 (STRIC_1750) comGG 168824..169108 (+) 285 WP_162097589.1 competence type IV pilus minor pilin ComGG -
  STRIC_RS08555 (STRIC_1751) comYH 169157..170110 (+) 954 WP_008089827.1 class I SAM-dependent methyltransferase Machinery gene
  STRIC_RS08560 (STRIC_1752) - 170167..171360 (+) 1194 WP_008089899.1 acetate kinase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35335.49 Da        Isoelectric Point: 6.9101

>NTDB_id=64582 STRIC_RS08520 WP_008089649.1 165492..166436(+) (comYA) [Streptococcus ictaluri 707-05]
MVQDLAKAIITQALALGAQDIYILPNIDGYYLWARVGSERKALDVDGCHRVANLISHFKYVAGMSVGEKRRCQLGACDYQ
LDKQKAVSLRLTTVGDYRGQESLVIRLLHDGQKELNYWFDDFSSLKEVIRGARGLYLFSGPVGSGKATLMHQLISETFQE
LQVISIEDPVEIKDERILQLQVNDSIGMTYDNLIKLSLRHRPDLLIIGEIRDQETARAAIRASMTGAIVFSTVHAKSIPG
VYGRLLELGISQAELENSLNLIAYQRFISGGALIDYSQRDFEKHSPRKWNQKIDQLFNSGHLTEQEARIEKIIA

Nucleotide


Download         Length: 945 bp        

>NTDB_id=64582 STRIC_RS08520 WP_008089649.1 165492..166436(+) (comYA) [Streptococcus ictaluri 707-05]
ATGGTTCAAGATTTAGCAAAAGCAATCATAACTCAAGCACTAGCGCTAGGGGCTCAGGATATTTATATTCTTCCTAATAT
AGACGGCTATTATCTTTGGGCAAGAGTAGGAAGTGAGAGGAAAGCTTTAGATGTGGATGGGTGTCATCGGGTGGCTAATC
TTATTAGTCACTTTAAATATGTTGCAGGAATGAGTGTCGGTGAAAAAAGACGTTGCCAGTTAGGGGCTTGCGATTACCAA
TTAGATAAGCAAAAGGCAGTTTCCTTACGTTTGACGACTGTTGGTGATTATCGTGGGCAAGAAAGTTTAGTCATTCGTTT
ACTACATGATGGGCAAAAGGAGCTAAACTATTGGTTTGACGACTTTTCTTCCCTAAAAGAGGTCATTAGAGGTGCCAGAG
GCCTATACCTCTTTTCAGGACCAGTGGGTTCTGGAAAAGCCACTTTGATGCACCAATTGATTTCGGAGACGTTTCAGGAA
TTACAGGTGATTAGTATTGAAGATCCAGTTGAAATCAAAGATGAGCGTATCTTGCAATTACAGGTTAATGATAGCATCGG
CATGACCTATGACAACTTGATTAAATTATCGTTGAGGCATCGACCAGATCTTTTGATTATTGGTGAAATCAGAGACCAAG
AAACAGCTAGGGCGGCTATAAGAGCGAGTATGACAGGAGCAATAGTCTTTTCAACTGTTCATGCCAAAAGCATTCCAGGA
GTTTATGGACGTCTTTTGGAACTTGGGATAAGTCAGGCAGAGTTGGAAAATAGTCTTAATTTAATCGCTTATCAGCGTTT
TATCAGTGGAGGAGCACTAATTGATTACAGTCAAAGAGACTTTGAAAAGCATAGCCCCCGAAAGTGGAATCAAAAAATTG
ACCAGCTTTTTAACTCAGGACATCTCACTGAGCAAGAGGCAAGGATCGAAAAAATTATCGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G5K4K7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

62.179

99.363

0.618
  comYA Streptococcus mutans UA140

62.179

99.363

0.618
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.058

99.045

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

61.218

99.363

0.608
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

60.577

99.363

0.602
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

60.577

99.363

0.602
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

60.577

99.363

0.602
  comGA/cglA/cilD Streptococcus pneumoniae D39

60.577

99.363

0.602
  comGA/cglA/cilD Streptococcus pneumoniae R6

60.577

99.363

0.602
  comGA Lactococcus lactis subsp. cremoris KW2

53.526

99.363

0.532
  comGA Latilactobacillus sakei subsp. sakei 23K

44.531

81.529

0.363

Multiple sequence alignment