Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LW136_RS17015 Genome accession   NZ_CP090366
Coordinates   3579830..3580297 (+) Length   155 a.a.
NCBI ID   WP_272877626.1    Uniprot ID   -
Organism   Stutzerimonas kunmingensis strain 7850S     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3574830..3585297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LW136_RS16990 (LW136_16990) coaE 3574963..3575571 (-) 609 WP_191121779.1 dephospho-CoA kinase -
  LW136_RS16995 (LW136_16995) pilD 3575703..3576572 (-) 870 WP_191121778.1 prepilin peptidase Machinery gene
  LW136_RS17000 (LW136_17000) pilC 3576575..3577792 (-) 1218 WP_191121777.1 type II secretion system F family protein Machinery gene
  LW136_RS17005 (LW136_17005) pilB 3577794..3579497 (-) 1704 WP_234173235.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LW136_RS17015 (LW136_17015) pilE 3579830..3580297 (+) 468 WP_272877626.1 pilin Machinery gene
  LW136_RS17020 (LW136_17020) tfpZ 3580510..3581244 (+) 735 WP_234173241.1 TfpX/TfpZ family type IV pilin accessory protein -
  LW136_RS17025 (LW136_17025) cysN 3581367..3583265 (-) 1899 WP_234173242.1 sulfate adenylyltransferase subunit CysN -
  LW136_RS17030 (LW136_17030) cysD 3583277..3584194 (-) 918 WP_014821370.1 sulfate adenylyltransferase subunit CysD -
  LW136_RS17035 (LW136_17035) - 3584380..3585138 (-) 759 WP_191121772.1 Nif3-like dinuclear metal center hexameric protein -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15776.22 Da        Isoelectric Point: 4.1316

>NTDB_id=641735 LW136_RS17015 WP_272877626.1 3579830..3580297(+) (pilE) [Stutzerimonas kunmingensis strain 7850S]
MKAQMQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEGMIAASSLKIGVTDVFADNGIAGVTAYSAVVAADQPN
LVTDLITAVAVDAGNGTISITMGGIPQLGGTNVLAFVPEINGAAIADNNSTGSITWDCGSATTTIIDKFLPANCR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=641735 LW136_RS17015 WP_272877626.1 3579830..3580297(+) (pilE) [Stutzerimonas kunmingensis strain 7850S]
ATGAAAGCTCAAATGCAGAAAGGCTTCACCCTGATTGAATTAATGATCGTCGTTGCGATCATCGGGATTTTGGCTGCAGT
AGCATTGCCGGCCTACCAAGACTACACAGTGCGTGCCAAGGTCTCGGAAGGCATGATCGCCGCTAGCTCACTTAAAATCG
GCGTAACTGATGTGTTTGCCGACAACGGAATCGCTGGTGTTACCGCCTACTCTGCAGTAGTTGCTGCAGATCAACCCAAC
CTCGTCACCGATCTAATTACCGCCGTTGCTGTTGATGCTGGCAATGGCACCATCTCAATTACCATGGGTGGGATTCCGCA
ACTTGGCGGAACCAACGTCCTAGCCTTCGTACCGGAGATCAACGGCGCCGCAATTGCAGATAACAACTCGACCGGTTCGA
TTACTTGGGATTGTGGTAGCGCCACAACCACCATCATTGATAAGTTCCTGCCAGCCAACTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

44.91

100

0.484
  comP Acinetobacter baylyi ADP1

43.312

100

0.439
  pilE Neisseria gonorrhoeae MS11

39.521

100

0.426
  pilA2 Legionella pneumophila str. Paris

43.421

98.065

0.426
  pilA2 Legionella pneumophila strain ERS1305867

42.763

98.065

0.419
  pilA Ralstonia pseudosolanacearum GMI1000

37.87

100

0.413
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.506

100

0.413
  pilE Neisseria gonorrhoeae strain FA1090

38.182

100

0.406
  pilA Pseudomonas aeruginosa PAK

36.196

100

0.381