Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LW136_RS17005 Genome accession   NZ_CP090366
Coordinates   3577794..3579497 (-) Length   567 a.a.
NCBI ID   WP_234173235.1    Uniprot ID   -
Organism   Stutzerimonas kunmingensis strain 7850S     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3572794..3584497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LW136_RS16975 (LW136_16975) - 3572978..3574015 (+) 1038 WP_191122335.1 NADP(H)-dependent aldo-keto reductase -
  LW136_RS16980 (LW136_16980) - 3574078..3574758 (+) 681 WP_042928463.1 energy-coupling factor ABC transporter permease -
  LW136_RS16985 (LW136_16985) yacG 3574769..3574966 (-) 198 WP_003302802.1 DNA gyrase inhibitor YacG -
  LW136_RS16990 (LW136_16990) coaE 3574963..3575571 (-) 609 WP_191121779.1 dephospho-CoA kinase -
  LW136_RS16995 (LW136_16995) pilD 3575703..3576572 (-) 870 WP_191121778.1 prepilin peptidase Machinery gene
  LW136_RS17000 (LW136_17000) pilC 3576575..3577792 (-) 1218 WP_191121777.1 type II secretion system F family protein Machinery gene
  LW136_RS17005 (LW136_17005) pilB 3577794..3579497 (-) 1704 WP_234173235.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LW136_RS17015 (LW136_17015) pilE 3579830..3580297 (+) 468 WP_272877626.1 pilin Machinery gene
  LW136_RS17020 (LW136_17020) tfpZ 3580510..3581244 (+) 735 WP_234173241.1 TfpX/TfpZ family type IV pilin accessory protein -
  LW136_RS17025 (LW136_17025) cysN 3581367..3583265 (-) 1899 WP_234173242.1 sulfate adenylyltransferase subunit CysN -
  LW136_RS17030 (LW136_17030) cysD 3583277..3584194 (-) 918 WP_014821370.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61659.72 Da        Isoelectric Point: 5.3394

>NTDB_id=641734 LW136_RS17005 WP_234173235.1 3577794..3579497(-) (pilB) [Stutzerimonas kunmingensis strain 7850S]
MSETIPLSGLARQMVLAGVLDEKAAQQAQVQALRSKLPLVTYLVQNRLAKGGVVAELASEQFGLALLDLSALDKDSLPKD
LVSEKLARQHRALPLAKRGNKLYVAVSDPANHQAVTDIQFSTGLNTEAILVEDDKLGDAIDKFFEGGSTGLEGLGDVDLD
GVDVETVSDDVKNDEAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAFRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLGEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=641734 LW136_RS17005 WP_234173235.1 3577794..3579497(-) (pilB) [Stutzerimonas kunmingensis strain 7850S]
ATGAGCGAAACTATTCCCCTATCCGGCTTGGCCCGGCAGATGGTGTTGGCAGGCGTGCTGGATGAGAAGGCCGCTCAGCA
GGCGCAGGTGCAAGCGTTGCGCAGCAAGCTGCCGCTAGTTACCTATCTGGTGCAGAACAGGCTTGCGAAGGGGGGGGTAG
TTGCCGAGCTTGCTTCCGAGCAGTTTGGTTTAGCGCTGCTGGATCTATCTGCGTTGGACAAGGACTCGCTGCCTAAGGAT
CTGGTCAGCGAAAAGTTGGCGCGCCAGCATCGAGCGCTACCGCTTGCAAAGCGAGGCAATAAGCTTTATGTGGCTGTTTC
TGATCCGGCTAACCATCAGGCGGTTACTGACATACAGTTCAGCACGGGGCTAAACACAGAAGCGATCCTGGTGGAGGACG
ACAAGCTTGGTGATGCAATCGACAAGTTCTTCGAGGGCGGTTCGACGGGGCTGGAGGGCTTGGGCGACGTCGATCTGGAC
GGTGTTGACGTCGAGACCGTGTCGGACGATGTAAAAAATGATGAGGCAGGTGAGGCGGCAGACGATGCCCCGGTTGTTCG
CTTTGTCAATAAGATGTTGCTTGACGCTATTCGCGGCGGCTCTTCTGACTTGCATTTCGAGCCTTATGAAAAGGCGTTTC
GCGTACGGCTCCGTACCGATGGCATTCTGCATGAGGTCGCGCGGCCGCCGATTCAACTGGCGCCGCGCATTTCTGCGCGC
CTAAAGGTGATGGCGGGGCTGGATATTTCCGAGCGTCGCAAGCCTCAGGACGGCCGTATCAAGATGAAACTGTCCAAGAG
CAAGGCAATTGATTTTCGCGTCAATACGCTGCCCACGCTTTGGGGCGAGAAGATCGTGATGCGAATCCTTGATCCGTCCA
GTGCACAAATGGGTATCGATGCATTGGGCTATGAAGAAGACCAGAAAGAGCTCTATATGAATGCCCTGAAGCAACCGCAG
GGCATGATCTTGGTCACCGGCCCGACCGGCTCTGGTAAGACTGTGTCCCTCTACACCGGGCTGAACATCCTGAATACGGT
CGATGTGAATATTTCGACCGCAGAAGATCCTGTAGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCACGCC
AGGGGATGGATTTCTCCCAGGCGCTGCGCGCGTTTCTGCGTCAGGATCCGGACATCATCATGGTAGGTGAGATTCGCGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACGGGGCACATGGTCATGTCGACGCTACACACCAACAGCGCCGC
GGAAACCCTGACCCGTCTTCGCAATATGGGGGTGGCCGCGTTCAATATCGCGACATCGGTCAATCTGATCATCGCGCAGC
GACTGGCACGCAAGCTTTGCAACAGTTGCAAGAAAGAGGTGGAGCTGCCGCGCGAGGCGCTGCTGGGCGAAGGTTTCCCT
GCCGATAAAATTGGGACGTTCAAGATCTACGGCCCGGTCGGCTGCGATAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCACTGGCCAGGATTATCATGGAAGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGACCTGCGTACATCGGCCCTAGTGAAGGCCATGCAGGGCGTGACAAGCCTGGAAGAA
GTGAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.574

99.471

0.593
  pilB Acinetobacter baylyi ADP1

59.22

99.471

0.589
  pilB Legionella pneumophila strain ERS1305867

54.545

100

0.55
  pilB Vibrio cholerae strain A1552

51.25

98.765

0.506
  pilB Vibrio campbellii strain DS40M4

49.645

99.471

0.494
  pilF Neisseria gonorrhoeae MS11

49.027

99.647

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

52.692

91.711

0.483
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.434

93.474

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362