Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA4_RS25575 Genome accession   NZ_CP104866
Coordinates   5504412..5504876 (-) Length   154 a.a.
NCBI ID   WP_003111216.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5499412..5509876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA4_RS25560 (PALA4_05056) nadC 5501932..5502780 (+) 849 WP_003110344.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA4_RS25570 (PALA4_05058) - 5502961..5504346 (-) 1386 WP_020751089.1 O-antigen ligase family protein -
  PALA4_RS25575 (PALA4_05059) pilA 5504412..5504876 (-) 465 WP_003111216.1 pilin Machinery gene
  PALA4_RS25580 (PALA4_05060) pilB 5505107..5506807 (+) 1701 WP_003111215.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA4_RS25585 (PALA4_05061) pilC 5506811..5508028 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PALA4_RS25590 (PALA4_05062) pilD 5508029..5508901 (+) 873 WP_003111214.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA4_RS25595 (PALA4_05063) coaE 5508898..5509509 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA4_RS25600 (PALA4_05064) yacG 5509506..5509706 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16253.62 Da        Isoelectric Point: 9.0066

>NTDB_id=634017 PALA4_RS25575 WP_003111216.1 5504412..5504876(-) (pilA) [Pseudomonas aeruginosa strain PALA4]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSTTPNDTQYDIGFTE
STLLDGSGKSQIKVTDNKNGTVEMVATLGKSSGSAIKGAVITVSRDAQGVWNCKISKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=634017 PALA4_RS25575 WP_003111216.1 5504412..5504876(-) (pilA) [Pseudomonas aeruginosa strain PALA4]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCACGACTCCTAACGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCAAGGTAACGGACAATAAAAATGGCACCGTTGAGATGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAACTGCA
AAATCTCCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

43.421

98.701

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.137

99.351

0.429
  pilA Pseudomonas aeruginosa PAK

41.667

100

0.422
  pilA Vibrio parahaemolyticus RIMD 2210633

38.312

100

0.383
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.364
  pilA Vibrio cholerae C6706

36.364

100

0.364
  pilA Vibrio cholerae strain A1552

36.364

100

0.364