Detailed information NTG0627150

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY56_RS15375
• Genome accession: NZ_CP087168
• Coordinates: 3303200..3304309 (-)
• Length: 369 a.a.
• NCBI ID: WP_258615293.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-048
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	3301593..3303014	3303200..3304309	flank	186

Gene organization within MGE regions

Location: 3301593..3304309

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY56_RS15370 (LOY56_15335)	-	3301593..3303014 (+)	1422	WP_258615292.1	IS1182 family transposase	-
LOY56_RS15375 (LOY56_15340)	pilU	3303200..3304309 (-)	1110	WP_258615293.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40970.83 Da
• Isoelectric Point: 6.5715

>NTDB_id=627150 LOY56_RS15375 WP_258615293.1 3303200..3304309(-) (pilU) [Pseudomonas sp. B21-048]
MEIDALLQILSSRNGSDLYLSTGAPPSARFDGVLKPLADQPFKPGEVAAIAASIMDAEQRLEFDRELEMNLALSLAGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLKLPPGLLETIMLKQGLILFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHAALKNTLRQSPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRTQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEIMLGTPTIGDLIRRNEFSELKGIMEKSSEAGMQTFDGAL
FELFAEGAIDEAEALKHADSVNNLRLRLKMHAEATPGPHTPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=627150 LOY56_RS15375 WP_258615293.1 3303200..3304309(-) (pilU) [Pseudomonas sp. B21-048]
ATGGAAATCGATGCACTGTTGCAGATTCTGTCCAGCCGCAATGGCTCCGATCTTTACCTGTCCACGGGAGCGCCACCCAG
CGCGCGATTCGACGGTGTGCTCAAACCCTTGGCCGATCAACCGTTCAAACCGGGAGAGGTGGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAACGCCTGGAGTTCGATCGCGAACTGGAAATGAACCTGGCGCTTTCTCTGGCTGGTGTCGGGCGC
TTTCGGCTCAATATCTTCAAGCAGCGCAACGATGTGTCCATCGTGGCGCGCAACATCAAACTCGACATTCCACGCTTCGA
AGACCTCAAACTGCCACCTGGGCTGCTTGAAACCATCATGCTCAAACAGGGGCTGATACTGTTCGTCGGTTCGACCGGCT
CAGGCAAGTCGACCTCGCTGGCGGCACTGATCGATTACCGCAACCGCCACAGCAGCGGGCATATCATCACCATCGAAGAC
CCCGTCGAGTTTATCCATCGGCACAAGAAATCGATCATCAATCAACGTGAGGTCGGCGTTGATACCCGCAGTTTTCATGC
GGCGTTGAAAAACACCCTGCGCCAGTCGCCGGACGTGGTGCTGATCGGCGAAATTCGTGACCGCGAGACTATGGAACATG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTCATTTCCACTTTGCATGCGCACAACGCCAATCAGGCGCTGGACCGCGTG
ATCAACTTCTTTCCCGAAGAACGCCGGACGCAACTGCTCCATGACCTGGGCAACAACCTCAAGGCCTTTGTTTCCCAGCG
GTTGGTGCGTACCCGCGATGGGCAACGCCGGGCGGCGGTGGAGATTATGCTGGGAACGCCGACCATCGGCGACCTGATCC
GGCGCAATGAGTTTTCCGAGCTCAAGGGGATTATGGAGAAGTCGTCAGAAGCGGGGATGCAGACGTTTGATGGGGCGCTG
TTCGAGCTATTTGCCGAGGGCGCGATTGATGAGGCAGAGGCGCTGAAACATGCGGATTCGGTGAACAACTTGCGCCTGCG
CCTGAAAATGCACGCCGAGGCTACGCCCGGGCCCCATACCCCGCCGGGTGAATGGGGTCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.804	99.729	0.537
pilU	Acinetobacter baylyi ADP1	52.247	96.477	0.504
pilU	Vibrio cholerae strain A1552	49.714	94.851	0.472
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.342	90.244	0.382
pilT	Vibrio cholerae strain A1552	42.342	90.244	0.382
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Pseudomonas aeruginosa PAK	40.116	93.225	0.374
pilT	Legionella pneumophila strain ERS1305867	41.317	90.515	0.374
pilT	Legionella pneumophila strain Lp02	41.317	90.515	0.374
pilT	Acinetobacter baumannii D1279779	40.12	90.515	0.363
pilT	Acinetobacter baumannii strain A118	40.12	90.515	0.363
pilT	Acinetobacter nosocomialis M2	39.82	90.515	0.36