Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VIBNI_RS17065 Genome accession   NC_022528
Coordinates   3769465..3771195 (-) Length   576 a.a.
NCBI ID   WP_022551972.1    Uniprot ID   -
Organism   Vibrio nigripulchritudo     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3764465..3776195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBNI_RS17045 (VIBNI_A3577) - 3765189..3766022 (-) 834 WP_004405319.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VIBNI_RS17050 (VIBNI_A3578) - 3766092..3767615 (-) 1524 WP_022551971.1 AAA family ATPase -
  VIBNI_RS17055 (VIBNI_A3579) aroB 3767646..3768734 (-) 1089 WP_004405321.1 3-dehydroquinate synthase -
  VIBNI_RS17060 (VIBNI_A3580) aroK 3768757..3769275 (-) 519 WP_004405322.1 shikimate kinase AroK -
  VIBNI_RS17065 (VIBNI_A3581) pilQ 3769465..3771195 (-) 1731 WP_022551972.1 type IV pilus secretin PilQ family protein Machinery gene
  VIBNI_RS17070 (VIBNI_A3582) pilP 3771232..3771744 (-) 513 WP_004405327.1 pilus assembly protein PilP Machinery gene
  VIBNI_RS17075 (VIBNI_A3583) pilO 3771737..3772324 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  VIBNI_RS17080 (VIBNI_A3584) pilN 3772321..3772881 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  VIBNI_RS17085 (VIBNI_A3585) pilM 3772874..3773890 (-) 1017 WP_004405331.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62367.66 Da        Isoelectric Point: 4.3158

>NTDB_id=62585 VIBNI_RS17065 WP_022551972.1 3769465..3771195(-) (pilQ) [Vibrio nigripulchritudo]
MAEFCRAHIYRCALLIFIGLFSTTGVHAQSNEFVGIDFRLSKEKHALLVVELASNAAAVDIQPMPDGLNISLHETSVVDE
KLFVVDVSDFATSVENVEVFREGSNTRLFASTNAAYNYDYRLRDRFLEITVSAKPKDEPEQASILEKEGKLISINFQDIP
VRSVLQLIADYNGFNLVVSDSVTGNLTLKVDDVPWQQVLDIILKVKGLDKRVEGNVILVAPKEEFDQREQQDLEQERLAQ
ELGTLESKIVEVKFAKASDIAEMIGGDGEISMLSERGSISIDERTNSLLVRDLPENISVIEDIVESLDIPVKQVQIEARI
VTVTEGELEELGVRWGFSSTSGPNSVGATIENNLATVGLYQAGGSGGDSGGEGGGSTSIDDFLAVNLGATNANAASIAFQ
VAKLGSDTLLDLELSALQTENKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATTVAFRKAVLSLKVTPQITPDNK
LVLDLSVTQDRPGKVVKTGNGEALAIETQRIGTQVLVDNGETVVLGGIYQQSVTNTVDKVPLLGDLPLLGALFRRSYENL
GKSELLIFITPKVVLQ

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=62585 VIBNI_RS17065 WP_022551972.1 3769465..3771195(-) (pilQ) [Vibrio nigripulchritudo]
ATGGCGGAGTTTTGTCGGGCGCATATATATCGTTGTGCTCTGCTTATCTTTATCGGGTTGTTTTCGACAACCGGGGTTCA
TGCCCAGTCCAACGAATTTGTTGGTATCGATTTTAGGCTCAGTAAAGAAAAACATGCTCTGCTGGTCGTGGAGTTAGCAT
CGAATGCGGCAGCGGTAGACATTCAGCCCATGCCAGATGGCTTAAACATCTCCCTGCATGAAACCAGCGTAGTGGACGAA
AAGCTTTTCGTGGTCGACGTATCCGATTTTGCAACTAGCGTAGAAAATGTGGAAGTGTTCCGTGAGGGCTCTAACACACG
TTTATTTGCCAGCACAAACGCGGCTTATAACTACGACTACCGACTACGGGATCGTTTTCTGGAAATCACCGTCTCTGCGA
AACCCAAAGACGAACCAGAGCAGGCGAGCATTCTAGAAAAAGAAGGCAAGCTTATTTCTATTAACTTCCAGGACATCCCT
GTTCGAAGCGTATTACAGCTTATCGCTGACTATAACGGGTTCAACCTGGTGGTATCTGACTCAGTCACAGGCAACTTAAC
GCTTAAGGTGGATGATGTGCCGTGGCAGCAAGTGCTAGACATCATCCTGAAAGTAAAAGGGTTGGATAAGCGTGTTGAAG
GCAATGTAATTCTGGTTGCACCAAAAGAAGAGTTTGACCAAAGAGAGCAGCAAGATCTTGAACAAGAACGTTTGGCGCAA
GAGCTCGGTACGCTGGAATCCAAAATCGTTGAAGTAAAATTTGCTAAAGCGTCTGACATTGCGGAAATGATCGGTGGCGA
CGGTGAAATTTCCATGCTTTCTGAGCGCGGAAGTATTTCCATTGATGAACGTACCAATTCCCTTCTGGTACGCGACTTGC
CGGAAAACATCAGCGTTATTGAAGATATTGTGGAATCTCTCGATATCCCTGTTAAACAGGTTCAGATAGAAGCTCGTATT
GTGACTGTCACAGAAGGTGAACTTGAAGAGTTGGGTGTCCGTTGGGGCTTCAGTTCAACAAGTGGACCAAACTCCGTGGG
CGCGACCATCGAAAATAATCTCGCGACGGTCGGTCTTTATCAGGCTGGTGGCTCAGGTGGTGATTCTGGAGGTGAAGGTG
GCGGCAGCACCAGTATTGATGACTTCCTTGCCGTCAACCTGGGGGCGACAAATGCCAATGCGGCCAGTATCGCGTTCCAA
GTGGCAAAACTTGGCTCTGACACCCTGTTGGATTTGGAGCTGTCTGCCTTGCAAACGGAAAACAAAGCGGAAATCATCTC
CAGCCCGAGACTGATTACCACCAACAAGAAACCGGCCTACATCGAGCAAGGTACTGAAATTCCTTACCTTGAAGCTTCAT
CCAGTGGTGCAACTACGGTCGCATTCCGTAAGGCTGTGCTGAGCTTAAAGGTAACGCCACAGATTACCCCAGACAACAAA
CTGGTGCTGGATTTGAGTGTTACTCAGGACAGACCCGGCAAAGTCGTAAAAACAGGCAATGGTGAAGCCCTGGCCATTGA
AACTCAAAGAATCGGTACTCAGGTGTTGGTAGACAATGGAGAAACGGTGGTTCTTGGGGGTATTTATCAACAAAGTGTGA
CAAATACGGTCGATAAAGTGCCACTTTTGGGTGATTTACCTCTGTTAGGTGCACTTTTCCGTCGAAGCTACGAAAATTTA
GGAAAAAGCGAACTCCTTATTTTCATTACGCCAAAGGTCGTTCTACAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(174-222)

Bacterial type II/III secretion system short domain (Secretin_N)

(248-313)

Bacterial type II and III secretion system protein (Secretin)

(416-574)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

72.958

95.66

0.698
  pilQ Vibrio cholerae strain A1552

67.009

100

0.681
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

67.009

100

0.681
  pilQ Legionella pneumophila strain ERS1305867

40.642

97.396

0.396
  pilQ Legionella pneumophila strain Lp02

40.642

97.396

0.396
  pilQ Pseudomonas aeruginosa PAK

39.964

96.007

0.384
  pilQ Acinetobacter baumannii D1279779

36.655

100

0.377
  pilQ Acinetobacter baumannii strain A118

36.655

100

0.377
  comQ Acinetobacter baylyi ADP1

38.053

98.09

0.373

Multiple sequence alignment