Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VIBNI_RS17085 Genome accession   NC_022528
Coordinates   3772874..3773890 (-) Length   338 a.a.
NCBI ID   WP_004405331.1    Uniprot ID   A0AAV2VI73
Organism   Vibrio nigripulchritudo     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3767874..3778890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBNI_RS17060 (VIBNI_A3580) aroK 3768757..3769275 (-) 519 WP_004405322.1 shikimate kinase AroK -
  VIBNI_RS17065 (VIBNI_A3581) pilQ 3769465..3771195 (-) 1731 WP_022551972.1 type IV pilus secretin PilQ family protein Machinery gene
  VIBNI_RS17070 (VIBNI_A3582) pilP 3771232..3771744 (-) 513 WP_004405327.1 pilus assembly protein PilP Machinery gene
  VIBNI_RS17075 (VIBNI_A3583) pilO 3771737..3772324 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  VIBNI_RS17080 (VIBNI_A3584) pilN 3772321..3772881 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  VIBNI_RS17085 (VIBNI_A3585) pilM 3772874..3773890 (-) 1017 WP_004405331.1 type IV pilus assembly protein PilM Machinery gene
  VIBNI_RS17090 (VIBNI_A3586) - 3774036..3776534 (+) 2499 WP_022551973.1 PBP1A family penicillin-binding protein -
  VIBNI_RS17095 (VIBNI_A3587) oxyR 3776590..3777471 (-) 882 WP_004405335.1 DNA-binding transcriptional regulator OxyR -
  VIBNI_RS17100 (VIBNI_A3588) - 3777650..3778381 (+) 732 WP_004405337.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37082.32 Da        Isoelectric Point: 6.4160

>NTDB_id=62589 VIBNI_RS17085 WP_004405331.1 3772874..3773890(-) (pilM) [Vibrio nigripulchritudo]
MGRSLVVGIDFGHHSIKAVAMKPIGELMQLVGYNELTVPEDIFSENHTLDYQKIVKKLKELKKGLPLFSQQVAVSVPDKA
VISKVLQIEQDMDGREQEFAVHQAFGQQSPIPLEELSLDFFEVDQKVTTRSTTSTFQVYATRKEVVDSRVAALTKAGLKP
LTVDVHANSLLKVWRLASQVHPERRQWMLVDIGLNQTSLCTVAAGRTPFYKEVPFGTQNVAQSQEVSESISLSSDSLTQG
FVRELGEKLQRHISLYNSVNPQQNVDGIWLSGGGANLVGIADMLSSELSLEVAQLNPFGLVENGTKKSIKQLSDSAAYGV
AMGLAISGVEWLKGNHHA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=62589 VIBNI_RS17085 WP_004405331.1 3772874..3773890(-) (pilM) [Vibrio nigripulchritudo]
ATGGGTAGATCTTTAGTAGTTGGCATCGATTTCGGTCATCACAGTATCAAAGCTGTCGCCATGAAACCCATTGGCGAGCT
AATGCAACTTGTTGGTTATAATGAGCTTACTGTACCCGAAGATATTTTCTCTGAAAACCATACGCTCGATTATCAGAAGA
TTGTAAAGAAACTCAAAGAACTTAAAAAGGGCTTGCCTCTTTTTAGTCAACAAGTCGCAGTTTCCGTACCAGACAAGGCT
GTTATCAGCAAAGTTCTGCAAATTGAACAGGATATGGATGGCAGAGAACAAGAGTTTGCCGTTCATCAGGCTTTTGGCCA
GCAGTCTCCCATTCCATTGGAAGAATTGAGTTTAGACTTCTTCGAAGTGGACCAAAAAGTCACCACGCGCAGCACCACAT
CTACCTTTCAGGTTTATGCCACTCGTAAAGAAGTGGTCGATTCGCGAGTTGCGGCATTAACGAAGGCTGGGTTAAAACCG
TTGACGGTTGATGTACACGCCAACAGCCTACTAAAAGTCTGGCGTCTTGCCAGTCAGGTTCATCCGGAACGCCGACAGTG
GATGCTGGTGGATATCGGATTGAATCAAACGTCTTTATGCACGGTTGCCGCGGGAAGAACTCCTTTCTATAAAGAAGTGC
CTTTCGGTACTCAGAACGTGGCTCAGTCACAAGAAGTTTCTGAATCCATCTCTTTAAGCAGCGATTCTCTTACACAAGGG
TTTGTCAGAGAGTTAGGCGAAAAGCTGCAGCGCCACATTAGCCTATATAACTCCGTTAATCCTCAGCAAAACGTAGACGG
AATCTGGCTCAGTGGCGGCGGTGCCAATCTGGTTGGTATTGCGGACATGCTGAGCAGCGAGCTTTCCCTTGAAGTGGCAC
AACTGAACCCGTTTGGTCTAGTGGAAAACGGCACTAAGAAGTCGATAAAGCAGTTGTCTGATTCTGCCGCGTATGGTGTG
GCCATGGGGCTGGCAATCAGTGGAGTAGAATGGCTGAAAGGAAATCACCATGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-223)

Type IV pilus assembly protein PilM; (PilM_2)

(239-327)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.706

100

0.55
  pilM Vibrio campbellii strain DS40M4

44.97

100

0.45

Multiple sequence alignment