Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VIBNI_RS17080 Genome accession   NC_022528
Coordinates   3772321..3772881 (-) Length   186 a.a.
NCBI ID   WP_004405329.1    Uniprot ID   U4KA40
Organism   Vibrio nigripulchritudo     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3767321..3777881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBNI_RS17055 (VIBNI_A3579) aroB 3767646..3768734 (-) 1089 WP_004405321.1 3-dehydroquinate synthase -
  VIBNI_RS17060 (VIBNI_A3580) aroK 3768757..3769275 (-) 519 WP_004405322.1 shikimate kinase AroK -
  VIBNI_RS17065 (VIBNI_A3581) pilQ 3769465..3771195 (-) 1731 WP_022551972.1 type IV pilus secretin PilQ family protein Machinery gene
  VIBNI_RS17070 (VIBNI_A3582) pilP 3771232..3771744 (-) 513 WP_004405327.1 pilus assembly protein PilP Machinery gene
  VIBNI_RS17075 (VIBNI_A3583) pilO 3771737..3772324 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  VIBNI_RS17080 (VIBNI_A3584) pilN 3772321..3772881 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  VIBNI_RS17085 (VIBNI_A3585) pilM 3772874..3773890 (-) 1017 WP_004405331.1 type IV pilus assembly protein PilM Machinery gene
  VIBNI_RS17090 (VIBNI_A3586) - 3774036..3776534 (+) 2499 WP_022551973.1 PBP1A family penicillin-binding protein -
  VIBNI_RS17095 (VIBNI_A3587) oxyR 3776590..3777471 (-) 882 WP_004405335.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21363.47 Da        Isoelectric Point: 7.4657

>NTDB_id=62588 VIBNI_RS17080 WP_004405329.1 3772321..3772881(-) (pilN) [Vibrio nigripulchritudo]
MHSVNLLPWRDEMRARYRRQFFSYLAAAILAAVGVQWGVGVYFSQQMSVQEERNAELTRHISYLDQQLRGLSEVRDQHES
ILTRLRSVEALQIKRNKTTDFMSHLPDTIPPGVYVDKIEMNDFEVEVNGISDSTANITTMLDSMERSAKLADVEMHSIVS
GKKLFGKVFKTFKVSFIFAPIKEEKS

Nucleotide


Download         Length: 561 bp        

>NTDB_id=62588 VIBNI_RS17080 WP_004405329.1 3772321..3772881(-) (pilN) [Vibrio nigripulchritudo]
ATGCATAGCGTAAACTTACTGCCCTGGCGTGATGAAATGCGCGCCAGATACCGCCGCCAATTTTTTTCTTATCTGGCTGC
CGCGATACTCGCCGCAGTCGGGGTGCAGTGGGGCGTTGGCGTTTATTTCTCGCAGCAAATGAGTGTTCAGGAAGAACGCA
ATGCTGAGCTGACTCGCCACATCTCCTATTTAGATCAGCAACTACGCGGTTTGAGTGAGGTTCGCGATCAGCACGAGTCT
ATTCTTACCCGACTGCGCTCGGTCGAGGCGCTTCAAATCAAGCGTAACAAAACCACGGATTTCATGTCCCACCTGCCAGA
TACCATTCCACCGGGTGTTTACGTCGACAAAATTGAAATGAACGATTTTGAAGTTGAAGTGAATGGCATCAGTGACAGTA
CAGCCAATATCACCACAATGCTCGACAGTATGGAGCGATCCGCCAAGCTGGCAGATGTAGAAATGCACTCCATTGTCTCG
GGTAAGAAGCTGTTTGGCAAAGTATTCAAAACCTTCAAAGTATCCTTCATTTTTGCGCCTATCAAGGAAGAAAAATCATG
A

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U4KA40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

52.406

100

0.527
  pilN Vibrio cholerae strain A1552

53.591

97.312

0.522

Multiple sequence alignment