Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VIBNI_RS17075 Genome accession   NC_022528
Coordinates   3771737..3772324 (-) Length   195 a.a.
NCBI ID   WP_004405328.1    Uniprot ID   -
Organism   Vibrio nigripulchritudo     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3766737..3777324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIBNI_RS17055 (VIBNI_A3579) aroB 3767646..3768734 (-) 1089 WP_004405321.1 3-dehydroquinate synthase -
  VIBNI_RS17060 (VIBNI_A3580) aroK 3768757..3769275 (-) 519 WP_004405322.1 shikimate kinase AroK -
  VIBNI_RS17065 (VIBNI_A3581) pilQ 3769465..3771195 (-) 1731 WP_022551972.1 type IV pilus secretin PilQ family protein Machinery gene
  VIBNI_RS17070 (VIBNI_A3582) pilP 3771232..3771744 (-) 513 WP_004405327.1 pilus assembly protein PilP Machinery gene
  VIBNI_RS17075 (VIBNI_A3583) pilO 3771737..3772324 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  VIBNI_RS17080 (VIBNI_A3584) pilN 3772321..3772881 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  VIBNI_RS17085 (VIBNI_A3585) pilM 3772874..3773890 (-) 1017 WP_004405331.1 type IV pilus assembly protein PilM Machinery gene
  VIBNI_RS17090 (VIBNI_A3586) - 3774036..3776534 (+) 2499 WP_022551973.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22736.13 Da        Isoelectric Point: 4.6739

>NTDB_id=62587 VIBNI_RS17075 WP_004405328.1 3771737..3772324(-) (pilO) [Vibrio nigripulchritudo]
MIDWQELDFEDLPEWPLVAQILVLALLFLILQGVGSWFYLSPMKADLEQLKQKEQTLKSTLIIKTNKVAALPKLQEQLDE
LNSRYDYLLKQLPEQKELASMLASVNELGIQHGLTFTRIDWGKKQNQEFLYRLPLNIELTGTYENIGKFSAAIANLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVTDE

Nucleotide


Download         Length: 588 bp        

>NTDB_id=62587 VIBNI_RS17075 WP_004405328.1 3771737..3772324(-) (pilO) [Vibrio nigripulchritudo]
ATGATCGATTGGCAAGAACTTGATTTCGAAGACTTACCGGAATGGCCCCTGGTTGCACAGATTTTAGTGCTGGCGCTGTT
GTTTCTGATTCTTCAAGGAGTGGGCAGCTGGTTTTATCTCTCTCCCATGAAAGCCGATCTTGAGCAGCTTAAGCAGAAAG
AGCAGACACTCAAAAGCACCCTCATTATCAAAACCAATAAGGTAGCGGCACTGCCAAAATTGCAGGAGCAACTGGATGAG
CTCAATAGCCGTTATGACTATCTGTTAAAACAGCTTCCTGAACAGAAAGAGTTGGCAAGCATGCTCGCTTCTGTAAACGA
GCTGGGTATTCAGCACGGTTTGACGTTTACCCGTATCGATTGGGGTAAGAAGCAGAATCAGGAGTTTCTTTATCGTCTGC
CTCTGAATATTGAACTCACCGGAACTTACGAAAACATTGGTAAGTTCTCAGCGGCCATTGCCAACCTACCGCGGATCATA
AACTTCGACGATGTCGACTGGCAGCGGGTCAGTCAGGAAAGCAGCACCCTTCACTTCAGGGTAAGGGCGTACACCTATCA
GTTCAAACCGGAGGTGACCGATGAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

68.718

100

0.687
  pilO Vibrio campbellii strain DS40M4

66.316

97.436

0.646

Multiple sequence alignment