Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilA1   Type   Machinery gene
Locus tag   LLJ08_RS15915 Genome accession   NZ_CP086067
Coordinates   3411551..3411982 (+) Length   143 a.a.
NCBI ID   WP_179484726.1    Uniprot ID   -
Organism   Stutzerimonas chloritidismutans strain 6L11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3406551..3416982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJ08_RS15890 (LLJ08_15895) coaE 3406697..3407305 (-) 609 WP_179484722.1 dephospho-CoA kinase -
  LLJ08_RS15895 (LLJ08_15900) pilD 3407437..3408306 (-) 870 WP_179484723.1 prepilin peptidase Machinery gene
  LLJ08_RS15900 (LLJ08_15905) pilC 3408309..3409526 (-) 1218 WP_179484724.1 type II secretion system F family protein Machinery gene
  LLJ08_RS15905 (LLJ08_15910) pilB 3409528..3411231 (-) 1704 WP_179484725.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LLJ08_RS15915 (LLJ08_15920) pilA/pilA1 3411551..3411982 (+) 432 WP_179484726.1 pilin Machinery gene
  LLJ08_RS15920 (LLJ08_15925) cysN 3412348..3414246 (-) 1899 WP_228207446.1 sulfate adenylyltransferase subunit CysN -
  LLJ08_RS15925 (LLJ08_15930) cysD 3414258..3415175 (-) 918 WP_228207447.1 sulfate adenylyltransferase subunit CysD -
  LLJ08_RS15930 (LLJ08_15935) - 3415361..3416119 (-) 759 WP_228207448.1 Nif3-like dinuclear metal center hexameric protein -

Sequence


Protein


Download         Length: 143 a.a.        Molecular weight: 14317.19 Da        Isoelectric Point: 4.4882

>NTDB_id=622286 LLJ08_RS15915 WP_179484726.1 3411551..3411982(+) (pilA/pilA1) [Stutzerimonas chloritidismutans strain 6L11]
MKAQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTARAQATEGLSATAGVRAEIASQLAVDGAVPGAGSQADTDAQALA
GNYFAAGGATVGANGQISVVFNAGTLSGQTMTITPTVDATSNQIQGWTCAGLTNTSHIPSGCR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=622286 LLJ08_RS15915 WP_179484726.1 3411551..3411982(+) (pilA/pilA1) [Stutzerimonas chloritidismutans strain 6L11]
ATGAAAGCTCAGATGCAGAAGGGTTTTACTCTTATCGAACTGATGATTGTCGTCGCGATCATTGGCATCCTGGCAGCGAT
TGCACTTCCGGCCTATCAAGACTACACCGCTCGCGCACAAGCAACTGAAGGGCTTTCTGCCACTGCAGGCGTGCGAGCAG
AAATCGCTAGCCAACTGGCAGTCGATGGCGCAGTTCCTGGCGCTGGTTCGCAAGCAGATACTGACGCGCAAGCGTTGGCA
GGCAATTATTTTGCTGCAGGCGGTGCAACTGTTGGTGCTAACGGGCAAATCTCGGTCGTTTTTAACGCCGGTACGCTTTC
TGGCCAAACGATGACTATCACCCCGACAGTTGATGCTACCTCGAACCAGATCCAAGGCTGGACCTGCGCTGGCTTGACAA
ATACTTCTCATATTCCGTCGGGCTGCCGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

55.556

100

0.594

  pilA2 Legionella pneumophila str. Paris

46.763

97.203

0.455

  pilA2 Legionella pneumophila strain ERS1305867

43.885

97.203

0.427

  pilA Ralstonia pseudosolanacearum GMI1000

44.118

95.105

0.42

  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.096

100

0.42

  pilE Neisseria gonorrhoeae strain FA1090

35.976

100

0.413

  pilA Pseudomonas aeruginosa PAK

36.709

100

0.406

  comP Acinetobacter baylyi ADP1

39.041

100

0.399

  pilA Acinetobacter baumannii strain A118

38.129

97.203

0.371