Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LLJ08_RS15905 Genome accession   NZ_CP086067
Coordinates   3409528..3411231 (-) Length   567 a.a.
NCBI ID   WP_179484725.1    Uniprot ID   -
Organism   Stutzerimonas chloritidismutans strain 6L11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3404528..3416231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJ08_RS15875 (LLJ08_15880) - 3404711..3405748 (+) 1038 WP_179484720.1 NADP(H)-dependent aldo-keto reductase -
  LLJ08_RS15880 (LLJ08_15885) - 3405812..3406492 (+) 681 WP_042928463.1 energy-coupling factor ABC transporter permease -
  LLJ08_RS15885 (LLJ08_15890) yacG 3406503..3406700 (-) 198 WP_179484721.1 DNA gyrase inhibitor YacG -
  LLJ08_RS15890 (LLJ08_15895) coaE 3406697..3407305 (-) 609 WP_179484722.1 dephospho-CoA kinase -
  LLJ08_RS15895 (LLJ08_15900) pilD 3407437..3408306 (-) 870 WP_179484723.1 prepilin peptidase Machinery gene
  LLJ08_RS15900 (LLJ08_15905) pilC 3408309..3409526 (-) 1218 WP_179484724.1 type II secretion system F family protein Machinery gene
  LLJ08_RS15905 (LLJ08_15910) pilB 3409528..3411231 (-) 1704 WP_179484725.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LLJ08_RS15915 (LLJ08_15920) pilA/pilA1 3411551..3411982 (+) 432 WP_179484726.1 pilin Machinery gene
  LLJ08_RS15920 (LLJ08_15925) cysN 3412348..3414246 (-) 1899 WP_228207446.1 sulfate adenylyltransferase subunit CysN -
  LLJ08_RS15925 (LLJ08_15930) cysD 3414258..3415175 (-) 918 WP_228207447.1 sulfate adenylyltransferase subunit CysD -
  LLJ08_RS15930 (LLJ08_15935) - 3415361..3416119 (-) 759 WP_228207448.1 Nif3-like dinuclear metal center hexameric protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61906.93 Da        Isoelectric Point: 5.2436

>NTDB_id=622285 LLJ08_RS15905 WP_179484725.1 3409528..3411231(-) (pilB) [Stutzerimonas chloritidismutans strain 6L11]
MSENISLSGLARQVVGAGVLDERTAQQAQAQALRSKTPFVTYVVQNRLVKGRVIAELASEQFGVALMDLGALDKEVLPKD
LISEKLAKQHRVLPLSKRGTKLFVAISDPANHQSIADIQFSTGLSIEPVLVEDDKLGEAIEKLYDNGATGLEDLGDVDLD
GVDVESVSDDAKGDETGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKNKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=622285 LLJ08_RS15905 WP_179484725.1 3409528..3411231(-) (pilB) [Stutzerimonas chloritidismutans strain 6L11]
ATGAGCGAAAACATTTCTCTATCCGGACTGGCACGACAGGTGGTCGGTGCCGGGGTGCTAGACGAGAGAACTGCGCAGCA
GGCCCAAGCACAAGCGCTTCGCAGCAAAACGCCTTTCGTTACCTATGTTGTTCAGAACAGGCTGGTCAAGGGGCGCGTCA
TAGCCGAGCTTGCGTCTGAGCAGTTTGGCGTCGCATTGATGGACCTGGGCGCCCTGGATAAGGAGGTACTGCCCAAGGAT
CTGATCAGTGAGAAGCTTGCCAAACAGCATCGCGTGCTCCCCCTTTCCAAGCGTGGAACCAAGTTGTTCGTCGCTATTTC
TGACCCAGCGAATCATCAGTCCATTGCCGATATCCAGTTCAGTACCGGGCTTTCGATCGAGCCGGTCTTGGTAGAGGACG
ACAAGCTCGGTGAGGCAATCGAAAAGCTTTACGACAACGGTGCTACCGGGCTGGAGGATCTCGGCGATGTTGACCTGGAT
GGCGTGGACGTCGAGTCGGTCAGTGATGATGCTAAGGGCGATGAAACGGGCGAGGCCGCAGATGATGCGCCAGTGGTTCG
TTTCGTCAACAAGATGCTGCTTGATGCCATTCGTGGAGGCTCATCTGACCTTCATTTCGAACCGTACGAGAAAGCCTATC
GGGTACGGCTGCGTACTGATGGCATCCTGCATGAGGTGGCGCGTCCGCCAATACAGCTAGCGCCGAGGATTTCTGCGCGC
CTCAAGGTGATGGCGGGGCTGGATATTTCCGAGCGCCGCAAGCCGCAGGATGGTCGCATCAAGATGAAGCTGTCCAAGAA
CAAGGCCATCGATTTTCGCGTCAATACGCTACCCACGCTCTGGGGGGAGAAGATCGTGATGCGAATTCTCGATCCGTCCA
GTGCGCAGATGGGCATCGATGCATTGGGTTATGAAGAGGATCAGAAAGAGCTCTATATGAATGCCCTGAAGCAACCACAG
GGCATGATTTTGGTTACCGGCCCGACCGGCTCTGGTAAGACGGTTTCTCTCTACACCGGCCTAAATATTCTCAATACGGT
GGACGTGAATATATCTACTGCCGAAGATCCGGTGGAAATCAATCTCGAGGGCATCAACCAGGTCAACGTCAATCCTCGTC
AGGGGATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTACGTCAGGATCCAGACATCATCATGGTCGGTGAGATCCGCGAT
CTGGAGACCGCTGAGATTGCCATCAAGGCGGCGCAGACGGGCCACATGGTCATGTCGACGCTACACACCAACAGCGCCGC
GGAAACCCTGACCCGTCTTCGCAACATGGGGGTGGCCGCGTTCAATATCGCGACCTCGGTCAACCTGATCATCGCGCAGC
GACTGGCACGCAAGCTTTGCAACAGTTGCAAGAAGGAGATCGATCTGCCGCGCGAGGCGCTGTTGGAAGAGGGCTTCCCT
GCCGACAAGATCGGGACGTTCAAAATCTATGGTCCGGTCGGCTGCGATAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCACTGGCCAGGATTATCATGGAAGACGGCAACTCCATTGATATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTACATCGGCCCTAGTGAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTGAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.325

99.295

0.589
  pilB Legionella pneumophila strain ERS1305867

54.196

100

0.547
  pilB Vibrio cholerae strain A1552

51.282

96.296

0.494
  pilF Neisseria gonorrhoeae MS11

48.142

99.647

0.48
  pilB Vibrio campbellii strain DS40M4

47.636

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

50.766

92.063

0.467