Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SCD_RS00370 Genome accession   NC_022357
Coordinates   83283..84419 (+) Length   378 a.a.
NCBI ID   WP_009207132.1    Uniprot ID   S6AAP1
Organism   Sulfuricella denitrificans skB26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 78283..89419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCD_RS00335 (SCD_02448) - 78370..78618 (-) 249 WP_009207140.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  SCD_RS00340 (SCD_02447) - 78662..79681 (-) 1020 WP_009207139.1 hypothetical protein -
  SCD_RS00345 (SCD_02446) - 79678..79977 (-) 300 WP_009207138.1 DUF167 domain-containing protein -
  SCD_RS00350 (SCD_02445) - 79981..80556 (-) 576 WP_009207137.1 YggT family protein -
  SCD_RS00355 proC 80559..81388 (-) 830 Protein_69 pyrroline-5-carboxylate reductase -
  SCD_RS00360 (SCD_02442) - 81502..82191 (-) 690 WP_041673261.1 YggS family pyridoxal phosphate-dependent enzyme -
  SCD_RS00365 (SCD_02441) pilT 82228..83271 (+) 1044 WP_009207133.1 type IV pilus twitching motility protein PilT Machinery gene
  SCD_RS00370 (SCD_02440) pilU 83283..84419 (+) 1137 WP_009207132.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SCD_RS00375 (SCD_02439) - 84474..86138 (+) 1665 WP_009207131.1 long-chain fatty acid--CoA ligase -
  SCD_RS00380 (SCD_02438) hemJ 86125..86538 (-) 414 WP_009207130.1 protoporphyrinogen oxidase HemJ -
  SCD_RS00385 (SCD_02437) - 86591..87124 (+) 534 WP_232504420.1 TlpA disulfide reductase family protein -
  SCD_RS00390 (SCD_02436) - 87174..88780 (-) 1607 Protein_76 ABC-F family ATPase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42206.69 Da        Isoelectric Point: 6.5772

>NTDB_id=62183 SCD_RS00370 WP_009207132.1 83283..84419(+) (pilU) [Sulfuricella denitrificans skB26]
MERDQAVKFMHDLLRLMLSKKASDLFITADFPPAIKVDGKMTPVSNQRLTPQHTKELARSIMNDKQSSEFEASKECNFAI
SPADIGRFRVSAFIQQGRIGMVLRTITTEIPNFEDLGLPGVLKDVSMTKRGLVVFVGGTGSGKSTSLAAMIGYRNQNSYG
HIITIEDPVEYVHPHLNCILTQREVGVDTDSWHAALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCMSTLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLIPKVGGKGRVAAVEIMLNSPLIADLIFKGEVHEIKELMARSNELG
MITFDQALFKLYEADLINYEDALRNADSVNDLRLKIKLHGKATKDADLMSGLEHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=62183 SCD_RS00370 WP_009207132.1 83283..84419(+) (pilU) [Sulfuricella denitrificans skB26]
ATGGAACGCGATCAAGCAGTCAAATTCATGCACGACCTGTTGCGGTTGATGCTGTCGAAGAAGGCCTCGGATCTGTTTAT
CACCGCCGATTTCCCGCCTGCCATCAAGGTGGATGGCAAGATGACGCCGGTTTCCAATCAGAGGCTGACGCCGCAGCATA
CCAAGGAACTTGCGCGTTCAATCATGAACGACAAGCAGTCATCCGAGTTCGAGGCGAGCAAGGAGTGCAACTTCGCCATC
AGTCCGGCGGATATCGGACGTTTCCGCGTCAGCGCCTTTATCCAGCAGGGGCGGATCGGCATGGTGCTGCGCACCATCAC
CACCGAGATCCCCAATTTCGAAGATCTGGGGTTGCCTGGCGTGCTCAAGGACGTGTCGATGACCAAGCGCGGCCTGGTGG
TTTTTGTCGGCGGCACCGGTTCGGGGAAATCCACCTCCCTGGCTGCAATGATCGGCTACCGCAACCAGAACAGCTACGGC
CACATCATCACCATCGAGGATCCGGTCGAATACGTGCATCCGCACCTCAACTGTATCCTGACCCAGCGCGAGGTCGGCGT
GGATACCGATTCCTGGCATGCGGCGCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGATCCTGATTGGTGAGATCCGCG
ACCGCGAAACCATGGAATATGCCATCGCCTTCGCTGAAACCGGTCACTTGTGCATGTCTACTCTGCATGCCAACAGCGCT
AACCAGGCGCTCGACCGGATCATTAACTTCTTTCCCGAGGAACGGCGCCAGCAACTGCTGATGGACCTGTCCCTGAATCT
CAAGGCGTTCGTGTCGCAGCGCCTCATCCCCAAGGTTGGCGGCAAAGGACGGGTGGCGGCGGTCGAGATCATGCTCAACT
CGCCGCTGATTGCCGATCTGATCTTCAAGGGCGAGGTCCACGAGATCAAGGAGCTCATGGCCAGGTCCAACGAACTCGGT
ATGATCACTTTCGATCAGGCGTTGTTCAAGCTCTATGAGGCCGATCTGATCAACTACGAGGATGCGCTCAGAAACGCTGA
TTCTGTCAACGATCTGCGTCTCAAGATCAAGCTCCACGGCAAAGCTACCAAGGATGCCGACCTGATGAGCGGACTCGAAC
ATCTGGATATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AAP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.642

94.709

0.622
  pilU Acinetobacter baylyi ADP1

60.446

94.974

0.574
  pilU Vibrio cholerae strain A1552

57.771

90.212

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.478

88.624

0.394
  pilT Pseudomonas aeruginosa PAK

43.582

88.624

0.386
  pilT Legionella pneumophila strain Lp02

43.505

87.566

0.381
  pilT Legionella pneumophila strain ERS1305867

43.505

87.566

0.381
  pilT Acinetobacter baylyi ADP1

42.433

89.153

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.624

0.376
  pilT Acinetobacter baumannii strain A118

42.9

87.566

0.376
  pilT Acinetobacter baumannii D1279779

42.9

87.566

0.376
  pilT Acinetobacter nosocomialis M2

42.9

87.566

0.376

Multiple sequence alignment