Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NLL86_RS03505 Genome accession   NZ_CP100660
Coordinates   799281..799691 (+) Length   136 a.a.
NCBI ID   WP_254483065.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain ZL22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794281..804691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLL86_RS03470 (NLL86_03470) - 794302..794475 (-) 174 WP_008091951.1 DUF3094 domain-containing protein -
  NLL86_RS03475 (NLL86_03475) - 794608..795237 (-) 630 WP_027918232.1 DUF1780 domain-containing protein -
  NLL86_RS03480 (NLL86_03480) - 795324..796031 (+) 708 WP_254483061.1 energy-coupling factor ABC transporter permease -
  NLL86_RS03485 (NLL86_03485) yacG 796168..796368 (-) 201 WP_054886117.1 DNA gyrase inhibitor YacG -
  NLL86_RS03490 (NLL86_03490) coaE 796365..796988 (-) 624 WP_254483062.1 dephospho-CoA kinase -
  NLL86_RS03495 (NLL86_03495) pilD 796988..797854 (-) 867 WP_254483063.1 A24 family peptidase Machinery gene
  NLL86_RS03500 (NLL86_03500) pilC 797857..799065 (-) 1209 WP_254483064.1 type II secretion system F family protein Machinery gene
  NLL86_RS03505 (NLL86_03505) pilA 799281..799691 (+) 411 WP_254483065.1 pilin Machinery gene
  NLL86_RS03515 (NLL86_03515) - 799999..801321 (+) 1323 WP_254483067.1 integrase arm-type DNA-binding domain-containing protein -
  NLL86_RS03520 (NLL86_03520) - 802580..803488 (-) 909 WP_254483069.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13918.25 Da        Isoelectric Point: 6.9007

>NTDB_id=606029 NLL86_RS03505 WP_254483065.1 799281..799691(+) (pilA) [Pseudomonas fluorescens strain ZL22]
MKGQRGITLIELMIVVAIIGILATIAIPMYTNHQSRTKAAAGLLEISALKTAMELRLNEGKDVGSVAELGGQPATANCAI
AVSSKAADGIGTIACTLVDAPTNVVGKSLALTRSPTGWTCTTDIEEDLAPKGCKAP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=606029 NLL86_RS03505 WP_254483065.1 799281..799691(+) (pilA) [Pseudomonas fluorescens strain ZL22]
ATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATTGCCAT
CCCGATGTACACCAACCACCAGTCACGCACCAAGGCGGCGGCCGGGTTGCTGGAGATCAGCGCGCTGAAGACGGCAATGG
AGCTGCGGTTGAATGAAGGCAAGGATGTGGGGAGTGTGGCTGAGTTGGGCGGCCAGCCAGCTACGGCGAACTGTGCGATT
GCGGTCAGCAGCAAGGCGGCTGACGGGATTGGCACGATTGCATGCACCCTGGTTGATGCGCCGACCAATGTGGTTGGCAA
GAGCCTTGCCCTGACGCGTTCGCCAACTGGCTGGACCTGCACGACCGATATCGAGGAAGACCTCGCGCCGAAGGGCTGCA
AAGCGCCCTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

43.624

100

0.478
  pilA Acinetobacter baumannii strain A118

45.324

100

0.463
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.268

100

0.441
  pilA Vibrio cholerae C6706

40.268

100

0.441
  pilA Vibrio cholerae strain A1552

40.268

100

0.441
  comP Acinetobacter baylyi ADP1

38.816

100

0.434
  pilA Ralstonia pseudosolanacearum GMI1000

37.838

100

0.412
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

100

0.412
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

99.265

0.397
  pilA Vibrio parahaemolyticus RIMD 2210633

44.262

89.706

0.397